Inorganic/Organic Hybrid Silica Nanoparticles as a Nitric Oxide Delivery Scaffold

Abstract

The preparation and characterization of nitric oxide (NO)-releasing silica particles formed following the synthesis of N-diazeniumdiolate-modified aminoalkoxysilanes are reported. Briefly, an aminoalkoxysilane solution was prepared by dissolving an appropriate amount of aminoalkoxysilane in a mixture of ethanol, methanol, and sodium methoxide (NaOMe) base. The silane solution was reacted with NO (5 atm) to form N-diazeniumdiolate NO donor moieties on the amino-alkoxysilanes. Tetraethoxy-or tetramethoxysilane (TEOS or TMOS) was then mixed with different ratios of N-diazeniumdiolate-modified aminoalkoxysilane (10 -75 mol%, balance TEOS or TMOS). Finally, the silane mixture was added into ethanol in the presence of an ammonia catalyst to form NO donor silica nanoparticles via a sol-gel process. This synthetic approach allows for the preparation of NO delivery silica scaffolds with remarkably improved NO storage and release properties, surpassing all macromolecular NO donor systems reported to date with respect to NO payload (11.26μmol·mg −1 ), maximum NO release amount (357000 ppb·mg −1 ), NO release half-life (253 min), and NO release duration (101 h). The N-diazeniumdiolatemodified silane monomers and the resulting silica nanoparticles were characterized by 29 Si nuclear magnetic resonance (NMR) spectroscopy, UV-visible spectroscopy, chemiluminescence, atomic force microscopy (AFM), gas adsorption-desorption isotherms, and elemental analysis.

Fabrication of nitric oxide-releasing polyurethane glucose sensor membranes

“…Nitric oxide-releasing silica particles were synthesized based on the sol–gel process via the co-condensation of an aminosilane (i.e., MAP3, AEAP3, or AHAP3) or mercaptosilane (i.e., MPTMS) in a range of concentrations (65–75 mol%) with tetraethyoxysilane (TEOS) or tetramethoxysilane (TMOS) in similarly to those reported in previous studies (Riccio et al, 2011; Shin et al, 2007; Shin and Schoenfisch, 2008). Subsequent diazeniumdiolation of the amine-containing particles was performed under high pressure of NO for 3 d in the presence of sodium methoxide at room temperature.…”

Bactericidal Efficacy of Nitric Oxide-Releasing Silica Nanoparticles

“…24 Briefly, diazeniumdiolate NO donors were synthesized on aminoalkoxysilane precursors prior to nanoparticle construction (Scheme 1), enabling the formation of particles with superior NO release ability. By synthesizing diazeniumdiolatemodified aminoalkoxysilanes prior to nanoparticle synthesis, particle aggregation was reduced due to decreased hydrogen bonding interactions between amines.…”

trans‐Dinitrosyl‐Substituted Hexamolybdate and Study of Its Controllable NO Release

“…[1,2] The discovery of its activity in the cardiovascular system was recognized with the Nobel Prize in Medicine and led to an explosion of research in NO biology and chemistry. Recently, materials such as polymers [3][4][5] and polymers functionalized with silica nanoparticles [6,7] and zeolites [8] have all been used for storage and delivery of NO. Unfortunately, some of these NO donors suffer from drawbacks such as the generation of carcinogenic side products, low NO storage, and uncontrollable NO release, which may limit their applicability.…”