Inorganic nitrite increases the susceptibility of tilapia (Oreochromis niloticus) leucocytes to Streptococcus agalactiae

Zhao, Zaoya; Sun, Caiyun; Chen, Limin; Qin, Jingkai; Yuan, Xi; Li, Wensheng

doi:10.1016/j.fsi.2019.12.039

Cited by 5 publications

(2 citation statements)

References 62 publications

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“…Therefore, the highly enhanced oxidoreductase activity was not unexpected. 15 GO analysis showed that strain RC4 may metabolize nitrite by affecting its response to oxidative stress, transmembrane transport, response to stimulus, membrane, and oxidoreductase activity. 16,17 3.5.…”

Section: Identification Of Degs Under Different Nitritementioning

confidence: 99%

Transcriptomic Responses to Nitrite Degradation by Limosilactobacillus fermentum RC4 and Effect of ndh Gene Overexpression on Nitrite Degradation

Shi,

Che,

Sun

et al. 2023

J. Agric. Food Chem.

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The excessive nitrite residue may increase cell damage and cancer risk. Limosilactobacillu fermentum RC4 exhibited excellent nitrite degradation ability. Herein, the molecular mechanism of nitrite degradation by L. fermentum RC4 was studied by integrating scanning electron microscopy analysis, transcriptomics, and gene overexpression. The results demonstrated that the gene profile of RC4 cultured in MRS broth with 0, 100, and 300 mg/L NaNO 2 varied considerably; RC4 responded to nitrite degradation by regulating pyruvate metabolism, energy synthesis, nitrite metabolism, redox equilibrium, protein protection, and signaling. High nitrite concentrations affected the morphology of RC4 with a longer phenotype, rough and wrinkle cell and reduced cell surface hydrophobicity. Moreover, an up-regulated expression of gene ndh encoding NADH dehydrogenase, which provides electrons for nitrite reduction by catalyzing NADH, was identified when RC4 was exposed to nitrite. Overexpression of ndh in RC4 increased the nitrite degradation rate by 2−9.5% in MRS broth with 100 mg/L NaNO 2 . Thus, the findings of this study could be helpful for the application of L. fermentum to reduce nitrite residues and improve food safety in fermented food products.

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Section: Identification Of Degs Under Different Nitritementioning

confidence: 99%

Transcriptomic Responses to Nitrite Degradation by Limosilactobacillus fermentum RC4 and Effect of ndh Gene Overexpression on Nitrite Degradation

Shi,

Che,

Sun

et al. 2023

J. Agric. Food Chem.

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“…The research of De Gaetano et al (2018) suggested that S. agalactiae exploited vitronectin on the cell surface to trigger an internalization response and enter cells. It has been reported that S. agalactiae can generate enrichment of the proteins related to autophagy in tilapia leukocytes to affect autophagy (Zhao et al ., 2019). When autophagy occurs, the complex of Beclin1 and B-cell lymphoma 2 (Bcl2) will be dissociated, resulting in increased levels of Beclin1 and Bcl2 (Mukhopadhyay et al ., 2014).…”

mentioning

confidence: 99%

Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway

Geng

et al. 2022

Journal of Dairy Research

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Streptococcus agalactiae (S. agalactiae) infection is a significant cause of mastitis, resulting in loss of cellular homeostasis and tissue damage. Autophagy plays an essential function in cell survival, defense, and the preservation of cellular homeostasis, and is often part of the response to pathogenic challenge. However, the effect of autophagy induced by S. agalactiae in bovine mammary epithelial cells (bMECs) is mainly unknown. So in this study, an intracellular S. agalactiae infection model was established. Through evaluating the autophagy-related indicators, we observed that after S. agalactiae infection, a significant quantity of LC3-I was converted to LC3-II, p62 was degraded, and levels of Beclin1 and Bcl2 increased significantly in bMECs, indicating that S. agalactiae induced autophagy. The increase in levels of LAMP2 and LysoTracker Deep Red fluorescent spots indicated that lysosomes had participated in the degradation of autophagic contents. After autophagy was activated by rapamycin (Rapa), the amount of p-Akt and p-mTOR decreased significantly, whilst the amount of intracellular S. agalactiae increased significantly. Whereas the autophagy was inhibited by 3-methyladenine (3MA), the number of intracellular pathogens decreased. In conclusion, the results demonstrated that S. agalactiae could induce autophagy through PI3K/Akt/mTOR pathway and utilize autophagy to survive in bMECs.

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Transcriptome and metabolome profiling unveil the responses of crayfish to dietary astaxanthin

Zhao,

Zhou,

Zou

et al. 2023

Aquaculture Reports

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Inorganic nitrite increases the susceptibility of tilapia (Oreochromis niloticus) leucocytes to Streptococcus agalactiae

Cited by 5 publications

References 62 publications

Transcriptomic Responses to Nitrite Degradation by Limosilactobacillus fermentum RC4 and Effect of ndh Gene Overexpression on Nitrite Degradation

Transcriptomic Responses to Nitrite Degradation by Limosilactobacillus fermentum RC4 and Effect of ndh Gene Overexpression on Nitrite Degradation

Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway

Transcriptome and metabolome profiling unveil the responses of crayfish to dietary astaxanthin

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