Inorganic nanoparticles modify the phase behavior and viscoelastic properties of non-lamellar lipid mesophases

Mendozza, Marco; Caselli, Lucrezia; Montis, Costanza; Orazzini, Stefano; Carretti, Emiliano

doi:10.1016/j.jcis.2019.01.091

Cited by 14 publications

(13 citation statements)

References 52 publications

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“…the lipid bilayer, of lipid lyotropic liquid crystals [35]. Here, we directly embedded SPIONs into GMO/water bulk cubic phases, according to a protocol optimized in our previous studies [20,22,23](see section 2.3). We then characterized the thermotropic and magnetic behavior of the resulting hybrids.…”

Section: Spions-loaded Bulk Cubic Phasesmentioning

confidence: 99%

“…In the field of nanomedicine, the combination of SPIONs with liposomes is of particular interest, yielding to hybrid nanoparticles called "magnetoliposomes" [16][17][18][19]; such nano-hybrids have been proposed as multifunctional platforms for controlled drug delivery and MRI applications. By comparison, non-lamellar lipid scaffolds, such as lyotropic liquid crystals of cubic symmetry, have received far less attention [20][21][22][23]. The peculiar structure of cubic phases, which features extended hydrophilic and hydrophobic domains, maximizes the encapsulation efficiency of molecules of different polarities, such as glycolipids [24,25], fatty [26,27] and nucleic acids [28][29][30], nutrients and drugs [31].…”

Section: Introductionmentioning

confidence: 99%

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Lipid cubic mesophases combined with Superparamagnetic Iron Oxide Nanoparticles: a hybrid multifunctional platform with tunable magnetic properties for nanomedical applications

Caselli

Mendozza

Muzzi

et al. 2021

Preprint

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Hybrid materials composed of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) and lipid self-assemblies possess a considerable applicative potential in the biomedical field, specifically, for drug/nutrients delivery. In recent works we have shown that SPION-doped lipid cubic liquid crystals undergo a cubic-to-hexagonal phase transition under the action of temperature or of an alternating magnetic field (AMF). This transition triggers the release of drugs embedded in the lipid scaffold or in the water channels. In this contribution, we address this phenomenon in depth, to fully elucidate the structural details and optimize the design of hybrid multifunctional carriers for drug delivery. Combining Small-Angle X-Ray Scattering (SAXS) with a magnetic characterization, we find that in bulk lipid cubic phases the cubic-to-hexagonal transition determines the magnetic response of SPIONs. We then extend the investigation from bulk liquidcrystalline phases to colloidal dispersions, i.e. to lipid/SPIONs nanoparticles with cubic internal structure ("magnetocubosomes"). Through Synchrotron SAXS, we monitored the structural response of magnetocubosomes while exposed to an AMF: the magnetic energy, converted into heat by SPIONs, activates the cubic-to-hexagonal transition, and can thus be used as a remote stimulus to spike drug release "on-demand". In addition, we show that the AMF-induced phase transition in magnetocubosomes steers the re-alignment of SPIONs into linear string assemblies and connect this effect with the change in their magnetic properties, observed at the bulk level.Finally, we assess the internalization ability and cytotoxicity of magnetocubosomes in vitro on HT29 adenocarcinoma cancer cells, in order to test the applicability of these smart carriers in drug delivery applications.

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Section: Spions-loaded Bulk Cubic Phasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipid cubic mesophases combined with Superparamagnetic Iron Oxide Nanoparticles: a hybrid multifunctional platform with tunable magnetic properties for nanomedical applications

Caselli

Mendozza

Muzzi

et al. 2021

Preprint

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“…Recently Mendozza et al [59] found that hydrophobic NPs (3-3.6 nm) were encapsulated in liquid crystalline Phytantriol bilayers, promoting an NP-concentration dependent phase transition from a cubic to a hexagonal phase. Hickel et al [60] studied the influence of different antimicrobial peptides (length ~ 2.2-3.5 nm) on POPE mesophases.…”

Section: Introductionmentioning

confidence: 99%

Structural changes in lipid mesophases due to intercalation of dendritic polymer nanoparticles: Swollen lamellae, suppressed curvature, and augmented structural disorder

et al. 2020

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Understanding interactions between nanoparticles and model membranes is relevant to functional nano-composites and the fundamentals of nanotoxicity. In this study, the effect of polyamidoamine (PAMAM) dendrimers as model nanoparticles (NP) on the mesophase behaviour of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) has been investigated using high-pressure small-angle X-ray scattering (HP-SAXS). The pressuretemperature () diagrams for POPE mesophases in excess water were obtained in the absence and presence of G2 and G4 polyamidoamine (PAMAM) dendrimers (29 Å and 45 Å in diameter, respectively) at varying NP-lipid number ratio 0.0002-0.02) over the pressure range p = 1-3000 bar and temperature range T = 20-80 °C. The phase diagram of POPE exhibited the L β , L α and H II phases. Complete analysis of the phase diagrams, including the relative area pervaded by different phases, phase transition temperatures (T t ) and pressures (p t ), the lattice parameters (d-spacing), the pressure-dependence of d-spacing (d/p), and the structural ordering in the mesophase as gauged by the Scherrer coherence length (L) permitted insights into the size-and concentration-dependent interactions between the dendrimers and the model membrane system. The addition of dendrimers changed the phase transition pressure and temperature and resulted in the emergence of highly swollen lamellar phases, dubbed L β-den and L α-den . G4 PAMAM dendrimers at the highest concentration  = 0.02 suppressed the formation of the H II phase within the temperature range studied, whereas the addition of G2 PAMAM dendrimers at the same concentration promoted an extended mixed lamellar region in which L α and L β phases coexisted.

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“…In recent years the effects of additives on the phase diagram of cubic mesophases has started to be explored as a possible strategy to tune the arrangement of the lipid scaffold and to widen the dimension ranges of hydrophobic and hydrophilic domains, in order to better host and retain hydrophobic and hydrophilic drugs of different sizes, and/or tune the physical-chemical features of the liquid crystalline mesophases. For instance, fatty acids [ 23 , 24 ], phospholipids [ 25 , 26 ], photo-switchable molecules [ 27 , 28 , 29 ] or also nanoparticles, such as iron oxide [ 22 , 30 ], gold [ 31 ] and quantum-dots [ 32 ], have been shown to effectively modify the phase diagram of cubic mesophases, both in terms of lattice parameters and in terms of shift of the phase borders [ 33 ]. Recently, Mezzenga et al have reported on the swelling of monolinolein Pn3m modified by sugar esters [ 8 , 20 ]; the additive promotes a transition to Im3m cubic mesophase, with water nanochannels about 2.5 times larger than in pure lipid/water systems.…”

Section: Introductionmentioning

confidence: 99%

Controlling the Kinetics of an Enzymatic Reaction through Enzyme or Substrate Confinement into Lipid Mesophases with Tunable Structural Parameters

Mendozza¹,

Balestri²,

Montis³

et al. 2020

IJMS

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Lipid liquid crystalline mesophases, resulting from the self-assembly of polymorphic lipids in water, have been widely explored as biocompatible drug delivery systems. In this respect, non-lamellar structures are particularly attractive: they are characterized by complex 3D architectures, with the coexistence of hydrophobic and hydrophilic regions that can conveniently host drugs of different polarities. The fine tunability of the structural parameters is nontrivial, but of paramount relevance, in order to control the diffusive properties of encapsulated active principles and, ultimately, their pharmacokinetics and release. In this work, we investigate the reaction kinetics of p-nitrophenyl phosphate conversion into p-nitrophenol, catalysed by the enzyme Alkaline Phosphatase, upon alternative confinement of the substrate and of the enzyme into liquid crystalline mesophases of phytantriol/H2O containing variable amounts of an additive, sucrose stearate, able to swell the mesophase. A structural investigation through Small-Angle X-ray Scattering, revealed the possibility to finely control the structure/size of the mesophases with the amount of the included additive. A UV–vis spectroscopy study highlighted that the enzymatic reaction kinetics could be controlled by tuning the structural parameters of the mesophase, opening new perspectives for the exploitation of non-lamellar mesophases for confinement and controlled release of therapeutics.

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Inorganic nanoparticles modify the phase behavior and viscoelastic properties of non-lamellar lipid mesophases

Cited by 14 publications

References 52 publications

Lipid cubic mesophases combined with Superparamagnetic Iron Oxide Nanoparticles: a hybrid multifunctional platform with tunable magnetic properties for nanomedical applications

Lipid cubic mesophases combined with Superparamagnetic Iron Oxide Nanoparticles: a hybrid multifunctional platform with tunable magnetic properties for nanomedical applications

Structural changes in lipid mesophases due to intercalation of dendritic polymer nanoparticles: Swollen lamellae, suppressed curvature, and augmented structural disorder

Controlling the Kinetics of an Enzymatic Reaction through Enzyme or Substrate Confinement into Lipid Mesophases with Tunable Structural Parameters

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