Inorganic ions in spermathecal fluid and their transport across the spermathecal membrane of the queen bee, Apis mellifera

Gessner, B.; Gessner, K.

doi:10.1016/0022-1910(76)90212-2

Cited by 23 publications

(13 citation statements)

References 14 publications

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“…Similar cytological aspects already seen in the epithelial cells of the spermatheca in Periplaneta americana (Gupta & Smith 1969), Tenebrio molitor (Happ & Happ 1975), Apis mellifera (Dallai 1975) and Melanoplus sanguinipes (Ahmed & Gillot 1982), seem to be characteristic of cells involved in the ionic transport process (Berridge & Oschman 1972). For example, in A. mellifera, between the cells of the spermathecal wall and the lumen of the organ, there is a different ionic concentration maintained by active transport (Gessner & Gessner 1976). …”

Section: Discussionmentioning

confidence: 59%

Morphological and ultrastructural organization of the spermatheca of some Tettigoniidae (Insecta, Orthoptera)

Brundo

Longo

Sottile

et al. 2011

Italian Journal of Zoology

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A morphological and ultrastructural study of the spermatheca of some species of Orthoptera Tettigoniidae was carried out to understand the role of this female organ in the reproductive biology because no literature exists about it in this insect group. In all the examined species, the spermatheca is of ectodermal origin and is composed of a seminal receptacle, mainly involved in the collection and storage of the spermatozoa, and of a spermathecal duct. In both these organs, the epithelium of the wall is made up of two different cell types: cuticle-forming cells, underlying the cuticular intima, and gland cells. Both of these cell types have secretory features that differ among the species and also within the same species, in relation to the tracts examined. In particular, the ultrastructure of the distal tract of the spermathecal duct indicates a more marked secretory activity than in the other tracts of the duct. This activity is often accompanied by ultrastructural aspects, suggesting a lysis activity in both the epithelium and the upper cuticle. Based on these findings, it is hypothesized that the seminal receptacle and the spermathecal duct have different functional roles, despite having a similar general structural organization.

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Section: Discussionmentioning

confidence: 59%

Morphological and ultrastructural organization of the spermatheca of some Tettigoniidae (Insecta, Orthoptera)

Brundo

Longo

Sottile

et al. 2011

Italian Journal of Zoology

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“…Up to now physiological conditions were only studied in A. mellifera. The pH value of the spermathecal fluid is 8.6 (Gessner and Gessner, 1976). The stored spermatozoa have a reduced metabolism (Verma, 1973) which is thought to depend on the high pH.…”

Section: Discussionmentioning

confidence: 99%

“…For the long term storage of 3 up to 5 years in A. mellifera, secretions of the queen into the spermatheca seem to be of significance (Klenk et al, 2004). Further the spermathecal fluid contains several sugars and its pH value is 8.6 (Gessner and Gessner, 1976).…”

Section: Introductionmentioning

confidence: 99%

Instrumental insemination of Apis mellifera queens with hetero- and conspecific spermatozoa results in different sperm survival

et al. 2004

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-Sixty three queens of Apis mellifera were inseminated each with about 8 million spermatozoa from either 1 A. mellifera drone, 8 A. cerana, 5 A. dorsata or 20 A. florea drones. Spermatozoa were collected from vesiculae seminales, diluted in buffer and re-concentrated at 1,000 g for 10 minutes. Between 1.4% and 2.8% of the spermatozoa reached the spermatheca. Motility of spermatozoa of A. mellifera and A. cerana did not change within 4 weeks, it was nearly 100%. The motility of A. florea spermatozoa decreased to 83.4% after 3 days and to 33.9% after 4 weeks and motility of A. dorsata spermatozoa decreased to 61.2% after 3 days and to 26% after 4 weeks. Fertilization of A. mellifera eggs was 57% by A. mellifera spermatozoa. Calculation based on non-hatching eggs showed that about 40% were fertilized by A. cerana and A. florea and less than 20% by A. dorsata spermatozoa. The composition of spermathecal fluid seems to be different within the species and its significance for long term sperm storage is discussed. sperm survival / spermathecal fluid / hetero specific insemination / Apis mellifera queens / Apis species

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“…While the Na + concentrations in the hemolymph show the usual value for insect body fluids, the K + concentration in the spermathecal fluid is higher by about eightfold (Gessner and Gessner, 1976). The stored spermatozoa have a reduced metabolism (Verma, 1973) which is thought to depend on the high pH.…”

Section: Introductionmentioning

confidence: 99%

Proteins in spermathecal gland secretion and spermathecal fluid and the properties of a 29 kDa protein in queens of Apis mellifera

et al. 2004

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-One and two dimensional SDS-PAGE were used to characterize the protein pattern of the spermathecal gland secretion and spermathecal fluid in Apis mellifera queen pupae and emerged queens of different ages. The concentration of protein varied from 8.5 and 15.3 mg/mL in the spermathecal fluid, and from 5 to 8.5 mg/mL in the secretion. Development of the protein pattern of the gland secretion and spermathecal fluid was identical from pupae until the age of 3 days. In sexually mature queens (10 days or older) the gland secretion and spermathecal fluid each had one additional band at 79 kDa and at 29 kDa respectively. The 29 kDa protein was N-terminal blocked but several peptide fragments were sequenced after digestion with LysC protease. Only 2 of the sequences showed a distinct homology to the N-terminal half of the glycolytic enzyme triosephosphate isomerase (TPI). TPI antibody reacted with the 29 kDa protein, but the enzymatic activity was only 1/100 compared to TPI of hemolymph. The possible function of the protein is discussed. spermatheca / sperm storage / protein pattern / 29 kDa protein / Apis mellifera

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Inorganic ions in spermathecal fluid and their transport across the spermathecal membrane of the queen bee, Apis mellifera

Cited by 23 publications

References 14 publications

Morphological and ultrastructural organization of the spermatheca of some Tettigoniidae (Insecta, Orthoptera)

Morphological and ultrastructural organization of the spermatheca of some Tettigoniidae (Insecta, Orthoptera)

Instrumental insemination of Apis mellifera queens with hetero- and conspecific spermatozoa results in different sperm survival

Proteins in spermathecal gland secretion and spermathecal fluid and the properties of a 29 kDa protein in queens of Apis mellifera

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