Inorganic ion‐doped glass fibres as microspectrofluorimetric standards

Abstract

Uranyl and europium ion-doped fibres of 5-45 pm diameter were prepared which emit in the green and red spectral regions, respectively. Measurements were made of fluorescence flux as a function of fibre length, dopant concentration, slit shape and fibre diameter. The Aux was found to be proportional to fibre length and fluorophor concentration and proportional to the square of the fibre radius. In addition, the fluorescence flux as a functio,n of aperture size and shape and fibre position has been measured experimentally and compared to computer generated fluorescence flux envelopes. It is suggested that inorganic ion-doped fibres are suitable for use as microspectrofluorimetric standards.

How to Improve Quality Assurance in Fluorometry: Fluorescence-Inherent Sources of Error and Suited Fluorescence Standards

“…Other approaches are microdroplets of fluorescent solutions [92] or microcapillaries filled with fluorophore solutions [17,95,96]. Typical instrument standards are for instance inorganic ion-doped glass materials such as uranyl-containing glasses [97,98] that are of good photo-stability but no longer commercially available, or inorganic ion-doped fibers [99] developed for the calibration of the day-to-day instrument performance. Inorganic ion-doped glasses have only restricted application as reference standards for certain analytes with sufficiently similar excitation and emission spectra.…”

Characterization of instrumentation and calibrators for quantitative microfluorometry for immunofluorescence tests

“…Many investigators have used several kinds of emitters as calibrators for various applications (1,3,5,6,(11)(12)(13)(15)(16)(17)(18). Reference serum solutions of known reactivities are used as controls for the immunologic reactivities of fluorophore conjugates (3,10).…”

“…The NIST provided uranium-impregnated glass spun to 5to 10-»im-diameter fibers (16); U308 was incorporated as 2.48 weight % of the glass. These fibers give uniform yellow-green fluorescence throughout their length.…”