Inorganic Carbon Assimilation in Cyanobacteria: Mechanisms, Regulation, and Engineering

Hagemann, Martin; Song, Shanshan; Brouwer, Eva‐Maria

doi:10.1002/9783527824908.ch1

Cited by 18 publications

(24 citation statements)

References 140 publications

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“…Furthermore, the LC‐induced NDH1‐3 and constitutive NDH1‐4 complexes convert cellular CO 2 to HCO 3 − . Collectively, these C i uptake systems accumulate high cellular amounts of HCO 3 − , which then diffuses into the carboxysome, a protein‐coated bacterial micro‐compartment harboring carbonic anhydrase and RubisCO (reviewed by Rae et al ., 2013; Hagemann et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The expression of genes encoding C i uptake systems is induced under LC conditions, whereas genes encoding carboxysome proteins or enzymes of the CBBC and photorespiration are less regulated at the transcriptional level and may be subject to posttranscriptional regulation (Jablonsky et al ., 2016; Spät et al ., 2021). In cyanobacteria, three transcription factors, namely NdhR, CmpR, and CyAbrB2 regulate different sets of genes in response to changing C i conditions (reviewed in Hagemann et al ., 2021). NdhR and CmpR alter their DNA affinity by binding of low‐molecular‐mass effector molecules.…”

Section: Introductionmentioning

confidence: 99%

“…Sp€ at et al, 2021). In cyanobacteria, three transcription factors, namely NdhR, CmpR, and CyAbrB2 regulate different sets of genes in response to changing C i conditions (reviewed in Hagemann et al, 2021). NdhR and CmpR alter their DNA affinity by binding of low-molecular-mass effector molecules.…”

Section: Introductionmentioning

confidence: 99%

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The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO₂‐dependent gene expression

et al. 2022

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Summary The amount of inorganic carbon (Ci) fluctuates in aquatic environments. Cyanobacteria evolved a Ci‐concentrating mechanism (CCM) that is regulated at different levels. The regulator SbtB binds to the second messengers cAMP or c‐di‐AMP and is involved in acclimation to low Ci (LC) in Synechocystis sp. PCC 6803. Here, we investigated the role of SbtB and of associated second messengers at different Ci conditions. The transcriptome of wild‐type (WT) Synechocystis and the ΔsbtB mutant were compared with Δcya1, a mutant defective in cAMP production, and ΔdacA, a mutant defective in generating c‐di‐AMP. A defined subset of LC‐regulated genes in the WT was already changed in ΔsbtB under high Ci (HC) conditions. This response of ΔsbtB correlated with a diminished induction of many CCM‐associated genes after LC shift in this mutant. The Δcya1 mutant showed less deviation from WT, whereas ΔdacA induced CCM‐associated genes under HC. Metabolome analysis also revealed differences between the strains, whereby ΔsbtB showed slower accumulation of 2‐phosphoglycolate and ΔdacA differences among amino acids compared to WT. Collectively, these results indicate that SbtB regulates a subset of LC acclimation genes while c‐di‐AMP and especially cAMP appear to have a lesser impact on gene expression under different Ci availabilities.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO₂‐dependent gene expression

et al. 2022

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“…Bicarbonate then diffuses into the bacterial microcompartment carboxysome, where it is converted to CO 2 by carbonic anhydrase (CA) to generate a high concentration of CO 2 around Rubisco (Kaplan & Reinhold, 1999;Rae et al, 2013;Hagemann et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Operation of Carbon-Concentrating Mechanisms in Cyanobacteria and Algae requires altered poising of the Calvin-Benson cycle

Treves

Lucius

Feil

et al. 2022

Preprint

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Cyanobacteria and eukaryotic algae make a major contribution to global photosynthetic productivity. To cope with the low availability of CO2 in aqueous systems they deploy inorganic carbon-concentrating mechanisms (CCMs). These concentrate CO2 in microcompartments that contain Rubisco (carboxysomes in cyanobacteria; pyrenoids in green algae). The rest of the Calvin-Benson cycle (CBC) is located outside these microcompartments. We hypothesized that this physical separation requires modified poising of the CBC. Hence, Rubisco is physically separated from the other CBC enzymes outside these microcompartments. To test the hypothesis that this physical separation requires appropriate poising of the CBC, we profiled CBC metabolites under ambient CO2 in the cyanobacterium Synechocystis sp. PCC 6803 and three eukaryotic algae (Chlamydomonas reinhardtii, Chlorella sorokiniana, Chlorella ohadii). Comparison with recently reported profiles for a large set of terrestrial plants revealed that cyanobacteria and green algae have very distinctive CBC metabolite profiles, with low levels of pentose phosphates and, especially, high levels of ribulose 1,5-bisphosphate and 3-phosphoglycerate. We propose that large pools of the substrate and product of Rubisco are required to generate concentration gradients that drive movement into and out of the microcompartments. These observations raise questions about how CBC regulation was modified during the evolution of algal CCMs and their subsequent loss in terrestrial plants, and highlight that operation of CCMs requires co-evolution of the CBC.

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“…The CCM activity in model strains such as Synechocystis sp. PCC 6803 (hereafter Synechocystis ) is mainly regulated at the transcriptional level due to the action of several regulatory proteins such as NdhR, CmpR, CyAbrB2, and SbtB (reviewed in Hagemann et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The Entner-Doudoroff Pathway Contributes to Glycogen Breakdown During High to Low CO2 Shifts in the Cyanobacterium Synechocystis sp. PCC 6803

et al. 2021

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Cyanobacteria perform plant-like oxygenic photosynthesis to convert inorganic carbon into organic compounds and can also use internal carbohydrate reserves under specific conditions. A mutant collection with defects in different routes for sugar catabolism was studied to analyze which of them is preferentially used to degrade glycogen reserves in light-exposed cells of Synechocystis sp. PCC 6803 shifted from high to low CO2 conditions. Mutants defective in the glycolytic Embden–Meyerhof–Parnas pathway or in the oxidative pentose-phosphate (OPP) pathway showed glycogen levels similar to wild type under high CO2 (HC) conditions and were able to degrade it similarly after shifts to low CO2 (LC) conditions. In contrast, the mutant Δeda, which is defective in the glycolytic Entner-Doudoroff (ED) pathway, accumulated elevated glycogen levels under HC that were more slowly consumed during the LC shift. In consequence, the mutant Δeda showed a lowered ability to respond to the inorganic carbon shifts, displayed a pronounced lack in the reactivation of growth when brought back to HC, and differed significantly in its metabolite composition. Particularly, Δeda accumulated enhanced levels of proline, which is a well-known metabolite to maintain redox balances via NADPH levels in many organisms under stress conditions. We suggest that deletion of eda might promote the utilization of the OPP shunt that dramatically enhance NADPH levels. Collectively, the results point at a major regulatory contribution of the ED pathway for the mobilization of glycogen reserves during rapid acclimation to fluctuating CO2 conditions.

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Inorganic Carbon Assimilation in Cyanobacteria: Mechanisms, Regulation, and Engineering

Cited by 18 publications

References 140 publications

The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO₂‐dependent gene expression

The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO₂‐dependent gene expression

Operation of Carbon-Concentrating Mechanisms in Cyanobacteria and Algae requires altered poising of the Calvin-Benson cycle

The Entner-Doudoroff Pathway Contributes to Glycogen Breakdown During High to Low CO2 Shifts in the Cyanobacterium Synechocystis sp. PCC 6803

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Inorganic Carbon Assimilation in Cyanobacteria: Mechanisms, Regulation, and Engineering

Cited by 18 publications

References 140 publications

The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO2‐dependent gene expression

The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO2‐dependent gene expression

Operation of Carbon-Concentrating Mechanisms in Cyanobacteria and Algae requires altered poising of the Calvin-Benson cycle

The Entner-Doudoroff Pathway Contributes to Glycogen Breakdown During High to Low CO2 Shifts in the Cyanobacterium Synechocystis sp. PCC 6803

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The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO₂‐dependent gene expression

The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO₂‐dependent gene expression