Inoculum Build up of Bacterial Blight of Rice in Rice-Wheat Cropping Area of Punjab in Relation to Zero Tillage?

Bacterial blight caused by Xanthomonas oryzae pv. oryzae is one of the important bacterial diseases on rice. This disease causes typical symptoms on infected rice such as leaf blight which appears on leaves of young plants, after planting out, as pale-green to grey-green water-soaked streaks near the leaf tip and margins. In this research, during the period from 2004 to 2005, samples of infected plant were collected from different areas of Guilan province (Rasht, Lahijan, Foman, Anzaly, Talesh, Roudsarm Roudbar and Astara), to identify the causal agent of disease. For isolation of bacteria, infected tissue of leaves, stems and roots were crushed in pepton water then 100 µl of homogenate were cultured on nutrient agar (NA) and yeast dextrose carbonate (YDC) containing cyclohexamid antibiotic (50 µg/ml). Isolates of bacteria rod-shaped, gram negative bacteria and aerobic bacterium were obtained. The former isolates produced levan on media including sucrose. All isolates induced hypersensitive reaction (HR) on tobacco and geranium leaves. All of the isolated bacteria were oxidase, nitrate, urease, Tween 80 hydrolysis and indole negative and could not produce rot on potato tuber slices, produced H 2 S and grew in 36°C. The isolates could use citrate, L-lysin and cystein. The isolates produced acid from arabinose, galactose, myo-inositol, fruoctose, trehalose and mannose and hydrolyzed gelatin. Based on morphological, physiological, biochemical tests, PCR method with specific primers and pathogenicity properties, the predominant pathogenic type was identified as X. oryzae pv. oryzae. To the best of our knowledge, this is the first record of bacterial blight of rice in Iran.

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“…Rice (Oryza sativa) is one of the most important crops in the world (Akhtar et al 2003). It is the second major cereal crop of Iran after wheat.…”

Section: Introductionmentioning

confidence: 99%

“…The disease occurs at all stages of the rice crop and shows either streaks or leaf blight symptoms. If plant produces panicles, under zero tillage symptoms are essential (Akhtar et al 2003). The bacterium enters through of hydathodes and wounds on the roots or leaves.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Xathomonas Oryzae PV. Oryzae the Causal Agent of Bacterial Blight of Rice in Iran

Ghasemie

Kazempour

Padasht³

2008

Journal of Plant Protection Research

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Studies on Characteristics of Xanthomonas Oryzae Isolates Associated With Rice Crop

Nawaz

Tariq

Lodhi

et al. 2020

JOARPS

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Rice Bacterial Leaf Blight (BLB) is considered the most imperative disease among various dangerous maladies of rice in Pakistan. There is no any reliable source of resistance against this disease. Moreover, pathogen has a vast diversity in its population. So, first and most important step for its control is pathogen identification and characterization. Therefore, present studies were carried out for surveillance of disease and to collect disease specimens from Hyderabad and Tando-Muhammad Khan (TMK) Districts. Associated pathogen was isolated from collected samples by direct plating method. Fourteen cultures were purified by streaking method and were characterized on the basis of colony morphology, cell morphology and gram staining. The colonies were found to be large, medium and small. Their shapes were filamentous, irregular and circular. They were found to be raised, undulate, entire and convex showing pale yellow, yellow, off white, reddish and creamy color and surface of most of the colonies was observed to be smooth.

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Incidence of the Pink Noctuid Stem Borer, Sesamia inferens (Walker), on Wheat under Two Tillage Conditions and Three Sowing Dates in North-western Plains of India

Singh¹

2012

J. of Entomology

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Inoculum Build up of Bacterial Blight of Rice in Rice-Wheat Cropping Area of Punjab in Relation to Zero Tillage?

Cited by 3 publications

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Isolation and Identification of Xathomonas Oryzae PV. Oryzae the Causal Agent of Bacterial Blight of Rice in Iran

Isolation and Identification of Xathomonas Oryzae PV. Oryzae the Causal Agent of Bacterial Blight of Rice in Iran

Studies on Characteristics of Xanthomonas Oryzae Isolates Associated With Rice Crop

Incidence of the Pink Noctuid Stem Borer, Sesamia inferens (Walker), on Wheat under Two Tillage Conditions and Three Sowing Dates in North-western Plains of India

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