Inoculation with five diazotrophs alters nitrogen metabolism during the initial growth of sugarcane varieties with contrasting responses to added nitrogen

Santos, Silvana Gomes dos; Ribeiro, Flaviane da Silva; Alves, Gabriela Cavalcanti; Santos, Leandro Azevedo; Reis, Verônica Massena

doi:10.1007/s11104-019-04101-1

Cited by 17 publications

(17 citation statements)

References 56 publications

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“…The growth-promoting effect on sugarcane seedlings was observed as ea days after the inoculation of the bacterial strains at seedling formation stage, an tained through the cultivation period until 45 days after transplanting to the s growth-promotion has already been observed as a positive consequence of int between endophytic and diazotrophic bacteria and plants of the Poaceae family sugarcane [7,33], maize and wheat [5,32], or rice [34,35]. This effect has been obs several experiments, including some under field conditions [36][37][38].…”

Section: Discussionmentioning

confidence: 99%

“…N uptake from soil can occur either in the nitrate or ammonia forms or be provided by diazotrophic bacteria in the form of ammonium (NH 4 + ). Thus, N assimilation enzymes, such as nitrate reductase (NR) and glutamine synthetase, have been assessed to evaluate endophytic bacteria influence on N uptake and use efficiency, indicating activation of N metabolism and a higher uptake from soil [5][6][7]. Plant receptors of bacterial signals are known to recognize phytopathogenic bacteria or be involved in the identification of beneficial microorganisms by plants [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling

et al. 2021

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Beneficial plant–microbe interactions lead to physiological and biochemical changes that may result in plant-growth promotion. This study evaluated the effect of the interaction between sugarcane and endophytic bacterial strains on plant physiological and biochemical responses under two levels of nitrogen (N) fertilization. Six strains of endophytic bacteria, previously selected as plant growth-promoting bacteria (PGPB), were used to inoculate sugarcane mini stalks, with and without N fertilization. After 45 days, biomass production; shoot nutrient concentrations; foliar polyamine and free amino acid profiles; activities of nitrate reductase and glutamine synthase; and the relative transcript levels of the GS1, GS2, and SHR5 genes in sugarcane leaves were determined. All six endophytic strains promoted sugarcane growth, increasing shoot and root biomass, plant nutritional status, and the use efficiency of most nutrients. The inoculation-induced changes at the biochemical level altered the foliar free amino acid and polyamine profiles, mainly regarding the relative concentrations of citrulline, putrescine, glycine, alanine, glutamate, glutamine, proline, and aspartate. The transcription of GS1, GS2, and SHR5 was higher in the N fertilized seedlings, and almost not altered by endophytic bacterial strains. The endophytic strains promoted sugarcane seedlings growth mainly by improving nutrient efficiency. This improvement could not be explained by their ability to induce the production of amino acid and polyamine composts, or GS1, GS2, and SHR5, showing that complex interactions may be associated with enhancement of the sugarcane seedlings’ performance by endophytic bacteria. The strains demonstrated biotechnological potential for sugarcane seedling production.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling

et al. 2021

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“…The authors reported that the two sugarcane varieties, when inoculated with the bacterial mix, presented different results regarding the activity of enzymes related to the assimilation of N. Under low N concentration, nitrate reductase activity was increased in RB867515 by 26% in the shoots, and by 48% in the roots, while glutamine synthetase activity was 21% higher than the control. For the IACSP95-5000 under low N concentration, nitrate reductase activity decreased by 62% in roots, and glutamine synthetase activity was increased by 16% (Dos Santos et al 2019). This information corroborates with Schultz et al (2017), who analyzed yield parameters in two field sites and with two sugarcane varieties (RB867515 and RB72454) inoculated or the same bacterial mix.…”

Section: Main Inoculated Cropsmentioning

confidence: 99%

Microbial inoculants: reviewing the past, discussing the present and previewing an outstanding future for the use of beneficial bacteria in agriculture

2019

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More than one hundred years have passed since the development of the first microbial inoculant for plants. Nowadays, the use of microbial inoculants in agriculture is spread worldwide for different crops and carrying different microorganisms. In the last decades, impressive progress has been achieved in the production, commercialization and use of inoculants. Nowadays, farmers are more receptive to the use of inoculants mainly because high-quality products and multi-purpose elite strains are available at the market, improving yields at low cost in comparison to chemical fertilizers. In the context of a more sustainable agriculture, microbial inoculants also help to mitigate environmental impacts caused by agrochemicals. Challenges rely on the production of microbial inoculants for a broader range of crops, and the expansion of the inoculated area worldwide, in addition to the search for innovative microbial solutions in areas subjected to increasing episodes of environmental stresses. In this review, we explore the world market for inoculants, showing which bacteria are prominent as inoculants in different countries, and we discuss the main research strategies that might contribute to improve the use of microbial inoculants in agriculture.

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“…In the field, sugarcane can efficiently associate with nitrogen-fixing bacteria (Urquiaga et al, 2012;Beneduzi et al, 2013). The use of inoculants containing the selected bacterial isolates, for example, can increase the rates of fixed nitrogen (Schultz et al, 2014;Pereira et al, 2019) and alter the activity of nitrogen-assimilation enzymes (Santos et al, 2017(Santos et al, , 2019b, reducing the demands for nitrogenbased fertilizers, reducing the production costs. Besides fixing nitrogen, the bacteria associated with sugarcane can improve plant establishment in the field, acting through several mechanisms such as plant hormone production, insoluble nutrient (mainly phosphorus and iron) release, and biological control of pests and diseases, among others.…”

Section: Inoculação Eficiente De Bactérias Diazotróficas Via Sistema mentioning

confidence: 99%

“…Overall, inoculation by irrigation treatments in the field led to the best physiological conditions in sugarcane, indicating its better adaption to the environmental conditions of the Brazilian semiarid region. Alterations in sugarcane physiological behavior due to the inoculation of diazotrophic bacteria have already been assessed in greenhouse conditions (Medeiros et al, 2012;Santos et al, 2019b), but not in the field until now. Both in the cane-plant and ratooncane cycles, a lower net photosynthesis and stomatal conductance were observed in the negative control treatment, when sugarcane stomata were closed to reduce water loss, indicating a better adaptation of the inoculated plant, whose stomata was kept open, allowing gas exchange.…”

Section: Inoculação Eficiente De Bactérias Diazotróficas Via Sistema mentioning

confidence: 99%

Efficient inoculation of diazotrophic bacteria into sugarcane by the drip irrigation system

Simões

Oliveira

Souza

et al. 2019

Pesq. agropec. bras.

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The objective of this work was to assess the efficiency of the inoculation of a diazotrophic bacterial consortium by the drip irrigation system into sugarcane (Saccharum spp.), in the Brazilian semiarid region. The evaluated treatments were: standard inoculation by submersion; one or two inoculations by drip irrigation; nitrogen (urea) applied by fertigation; and negative controls, without inoculation or nitrogen fertilization. Leaf gas exchanges and plant growth and yield were assessed. Inoculation by irrigation is efficient for all variables, and double inoculation by the drip irrigation system stands out, increasing stem yield in 16% when compared with the negative control, without differing from the treatment with nitrogen fertilizer.

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Inoculation with five diazotrophs alters nitrogen metabolism during the initial growth of sugarcane varieties with contrasting responses to added nitrogen

Cited by 17 publications

References 56 publications

Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling

Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling

Microbial inoculants: reviewing the past, discussing the present and previewing an outstanding future for the use of beneficial bacteria in agriculture

Efficient inoculation of diazotrophic bacteria into sugarcane by the drip irrigation system

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