Inoculation of mares and very young foals with EHV-1 glycoproteins D and B reduces virus shedding following respiratory challenge with EHV-1

Foote, C.E.; Raidal, Sharanne; Pecenpetelovska, G.; Wellington, J. E.; Whalley, J. M.

doi:10.1016/j.vetimm.2006.01.012

Cited by 11 publications

(23 citation statements)

References 47 publications

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“…The sampling frequency used in the current study may have failed to detect nasal shedding or viremia, which is known to be of short duration in experimentally infected animals. 12 The finding in the present study that resident horses were infected with EHV-1 may have important implications for the breeding farm. Tissue samples for which results of the PCR assay were positive were obtained from the same mare that was found to have viral DNA in its nasal secretions at 159 days of gestation.…”

Section: Discussionmentioning

confidence: 55%

Prevalence of equine herpesvirus-1 infection among Thoroughbreds residing on a farm on which the virus was endemic

Brown

Mapes

Ball

et al. 2007

javma

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Section: Discussionmentioning

confidence: 55%

Prevalence of equine herpesvirus-1 infection among Thoroughbreds residing on a farm on which the virus was endemic

Brown

Mapes

Ball

et al. 2007

javma

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“…Thus, these proteins are major antigenic determinants for both humoral and cellular immune responses and have been utilized as subunit vaccines (2)(3)(4). Specifically, immunization with EHV-1 glycoprotein D (gD) has been shown to generate protective immune responses in mice and horses (3,(5)(6)(7)(8)(9)(10)(11). EHV-1 and herpes simplex virus 1 (HSV-1) glycoproteins D are required for entry into cells and cell-to-cell fusion, a function which is conserved in most but not all alphaherpesviruses (12).…”

mentioning

confidence: 99%

Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice

Liu

Stanfield

Chouljenko

et al. 2017

J Virol

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Vaccination remains the best option to combat equine herpesvirus 1 (EHV-1) infection, and several different strategies of vaccination have been investigated and developed over the past few decades. Herein, we report that the liveattenuated herpes simplex virus 1 (HSV-1) VC2 vaccine strain, which has been shown to be unable to enter into neurons and establish latency in mice, can be utilized as a vector for the heterologous expression of EHV-1 glycoprotein D (gD) and that the intramuscular immunization of mice results in strong antiviral humoral and cellular immune responses. The VC2-EHV-1-gD recombinant virus was constructed by inserting an EHV-1 gD expression cassette under the control of the cytomegalovirus immediate early promoter into the VC2 vector in place of the HSV-1 thymidine kinase (UL23) gene. The vaccines were introduced into mice through intramuscular injection. Vaccination with both the VC2-EHV-1-gD vaccine and the commercially available vaccine Vetera EHV XP 1/4 (Vetera; Boehringer Ingelheim Vetmedica) resulted in the production of neutralizing antibodies, the levels of which were significantly higher in comparison to those in VC2-and mock-vaccinated animals (P Ͻ 0.01 or P Ͻ 0.001). Analysis of EHV-1-reactive IgG subtypes demonstrated that vaccination with the VC2-EHV-1-gD vaccine stimulated robust IgG1 and IgG2a antibodies after three vaccinations (P Ͻ 0.001). Interestingly, Vetera-vaccinated mice produced significantly higher levels of IgM than mice in the other groups before and after challenge (P Ͻ 0.01 or P Ͻ 0.05). Vaccination with VC2-EHV-1-gD stimulated strong cellular immune responses, characterized by the upregulation of both interferonand tumor necrosis factor-positive CD4 ϩ T cells and CD8 ϩ T cells. Overall, the data suggest that the HSV-1 VC2 vaccine strain may be used as a viral vector for the vaccination of horses as well as, potentially, for the vaccination of other economically important animals.IMPORTANCE A novel virus-vectored VC2-EHV-1-gD vaccine was constructed using the live-attenuated HSV-1 VC2 vaccine strain. This vaccine stimulated strong humoral and cellular immune responses in mice, suggesting that it could protect horses against EHV-1 infection.KEYWORDS EHV-1, HSV-1, equine herpesvirus, live vector vaccines, glycoprotein D, immune response, mouse model

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“…Resultados similares aos observados no presente estudo foram descritos em um estudo de campo na Austrália, onde foi avaliada a soroconversão de 159 éguas e 101 potros após imunização com uma vacina contendo antígenos inativados do EHV-1. Mesmo seguindo criteriosamente as orientações do fabricante, menos de 30% das éguas e menos de 50% dos potros responderam à vacinação naquela ocasião (13) . Em relação aos testes sorológicos realizados com o EAV, foi observado que 5 amostras de soro foram consideradas positivas, representando 0,79% (5/630) do total de amostras analisadas.…”

Section: Resultsunclassified

Ocorrência De Anticorpos Contra O Herpesvírus Equino E Virus Da Arterite Equina Em Rebanhos Equinos Do Estado Do Rio De Janeiro

et al. 2015

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<title>Resumo</title><p>A infecção pelo herpesvírus equino (EHV) e o vírus da arterite equina (EAV) tem sido associada a perdas econômicas importantes para a equideocultura em nível mundial. Estudos sorológicos têm demonstrado a circulação destes agentes no Brasil. O presente trabalho teve como objetivo principal estudar a ocorrência de anticorpos específicos contra o EAV e EHV em cavalos oriundos de diferentes regiões do estado do Rio de Janeiro. Para este propósito, foram coletadas amostras de soro de 581 animais não vacinados e de 44 éguas provenientes de um plantel vacinado regularmente contra a rinopneunonite equina. Todas as amostras foram submetidas ao teste de soroneutralização para a pesquisa de anticorpos específicos contra cada um dos vírus. Os resultados demonstraram 29,6% (172/581) de animais soropositivos para o EHV (títulos entre 2 e ≥ 256) e 0,79% (05/630) para o EAV (títulos entre 2 e 4.096). Considerando a sorologia positiva em animais não imunizados, os achados demonstram a indução de anticorpos específicos após exposição natural aos respectivos vírus, indicando uma possível circulação desses agentes nos rebanhos analisados.</p>

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Inoculation of mares and very young foals with EHV-1 glycoproteins D and B reduces virus shedding following respiratory challenge with EHV-1

Cited by 11 publications

References 47 publications

Prevalence of equine herpesvirus-1 infection among Thoroughbreds residing on a farm on which the virus was endemic

Prevalence of equine herpesvirus-1 infection among Thoroughbreds residing on a farm on which the virus was endemic

Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice

Ocorrência De Anticorpos Contra O Herpesvírus Equino E Virus Da Arterite Equina Em Rebanhos Equinos Do Estado Do Rio De Janeiro

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