Inoculation of cranberry (<i>Vaccinium macrocarpon</i>) with the ericoid mycorrhizal fungus <i>Rhizoscyphus ericae </i>increases nitrate influx

Kosola, Kevin R.; Workmaster, Beth Ann; Spada, P.

doi:10.1111/j.1469-8137.2007.02149.x

Cited by 36 publications

(25 citation statements)

References 86 publications

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“…Our results thus disagree with the notion that NO 3 - availability is always negligible in acid soils (Paul and Clark, 1989) but support the claims of Scagel (2005) and Kosola et al (2007) that NO 3 - is an important N source for plants in the family Ericaceae. ERM colonization under acidic soil conditions probably plays a critical role for plants in the heather family to capture NO 3 - -N.…”

Section: Discussioncontrasting

confidence: 99%

“…An explanation is that cranberry has adapted to acidic soil conditions where pH ranges from 4 to 5 and nitrification is typically negligible at soil pH below 5.5 (Paul and Clark, 1989), therefore the adaptation has resulted in the loss of plant capacity to absorb NO 3 - . However, recent reports showed that inoculation with the fungus Rhizoscyphus ericae increased the capacity of cranberry to absorb NO 3 - (Kosola et al, 2007). Compared to uninoculated plants, inoculating certain blueberry ( Vaccinium corymbosum L.) cultivars with ERM fungi can increase nutrient concentration, particularly N, and plant growth (Scagel, 2005).…”

Section: Introductionmentioning

confidence: 99%

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A New Oidiodendron maius Strain Isolated from Rhododendron fortunei and its Effects on Nitrogen Uptake and Plant Growth

et al. 2016

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A new mycorrhizal fungal strain was isolated from hair roots of Rhododendron fortunei Lindl. grown in Huading Forest Park, Zhejiang Province, China. Morphological characterization and internal transcribed spacer rDNA analysis suggested that it belongs to Oidiodendron maius Barron, and we designated it as strain Om19. Methods for culturing Om19 were established, and the ability of Om19 to form mycorrhizae on R. fortunei was evaluated in a peat-based substrate. Microscopic observations showed hyaline hyphae on the surface of hair roots and crowded hyphal complexes (hyphal coils) inside root cortical cells of R. fortunei after inoculation, indicating that the roots were well colonized by Om19. In a second experiment, fresh and dry weight of R. fortunei 2 months after Om19 inoculation were greater than uninoculated plants, and the total nitrogen absorbed by plants inoculated with Om19 was greater than the uninoculated controls. qRT-PCR analysis of five genes related to N uptake and metabolism (two nitrate transporters, an ammonium transporter, glutamine synthetase, and glutamate synthase) showed that these genes were highly upregulated with twofold to ninefold greater expression in plants inoculated with Om19 compared to uninoculated plants. In the third experiment, Om19 was inoculated into the peat-based substrate for growing Formosa azalea (Rhododendron indica 'Formosa'). 'Formosa' azalea plants grown in the inoculated substrate had larger canopies and root systems compared to uninoculated plants. Our results show that Om19 could be an important microbial tool for improving production of Rhododendron plants.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Oidiodendron maius Strain Isolated from Rhododendron fortunei and its Effects on Nitrogen Uptake and Plant Growth

et al. 2016

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“…In the second greenhouse experiment, plants were transplanted to 4-L pots filled with the same peat and pine bark mix as in Experiment 1 and fertigated with ammonium sulfate or urea mixed in modified Johnson's nutrient solution (Kosola et al, 2007). The fertilizers were applied three times per week, weekly, or every 28 days.…”

Section: Methodsmentioning

confidence: 99%

Effects of Salinity Induced by Ammonium Sulfate Fertilizer on Root and Shoot Growth of Highbush Blueberry

Machado¹,

Bryla²,

Vargas³

2014

Acta Hortic.

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Ammonium sulfate fertilizer is commonly used in highbush blueberry (Vaccinium corymbosum L.) but often causes salt damage, particularly in young plants, when high rates are applied. Three experiments were done to determine the sensitivity of 'Bluecrop' blueberry to ammonium sulfate and identify the salinity threshold at which plant growth was affected. In the first experiment, plants were grown in pots and fertigated two to three times per week with 0, 0.25, 0.75, and 1.5 g·L -1 ammonium sulfate solution. Electrical conductivity of the solutions (EC w ) increased linearly with fertilizer rate and averaged 0.1, 0.5, 1.5, and 3.0 dS·m -1 , respectively. Plants fertigated with 1.5 g·L -1 ammonium sulfate produced less leaves and roots and had a lower leaf to stem dry weight ratio than those fertilized with 0 or 0.25 g·L -1 , which indicates that root and leaf growth in blueberry was sensitive to EC w > 1.5 dS·m -1 . In the second experiment, plants in pots were fertilized with ammonium sulfate or urea at a frequency of three times per week, weekly, or every 28 days, using the same total amount of nitrogen (N) in each treatment over a 4-week period. In this case, plant growth was higher with ammonium sulfate than with urea but also higher, regardless of fertilizer source, when plants were fertilized more frequently. In the third experiment, plants were grown in the field with no N fertilizer or with ammonium sulfate or urea applied by weekly fertigations or by a triple-split application of granular fertilizer at a total rate of 133 kg·ha -1 N during the third year after planting. Yield in the plants was greater with fertigation or with granular urea than with granular ammonium sulfate, the latter of which resulted in EC w levels in the soil solution as high as 13 dS·m -1 . In the field, fertilizer programs and practices such as fertigation that maintain EC w < 2 dS·m -1 are recommended for highbush blueberry.

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“…The adaptation to such soil conditions has resulted in the loss of the capacity to absorb NO 3 - . A recent report, however, showed that inoculation with the fungus Rhizoscyphus ericae increased the capacity of cranberry to absorb NO 3 - (Kosola et al, 2007), and the authors believe that NO 3 - may play a greater role in N nutrition of cranberry than previously thought and that high capacity for NO 3 - utilization is the ancestral state for the Ericaceae. Yin et al (2010) also found that ERM fungi significantly increased the ability of R. fortunei to absorb N, especially in the form of nitrate.…”

Section: Introductionmentioning

confidence: 99%

Differential Gene Expression in Rhododendron fortunei Roots Colonized by an Ericoid Mycorrhizal Fungus and Increased Nitrogen Absorption and Plant Growth

et al. 2016

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Ericoid mycorrhizal (ERM) fungi are specifically symbiotic with plants in the family Ericaceae. Little is known thus far about their symbiotic establishment and subsequent nitrogen (N) uptake at the molecular level. The present study devised a system for establishing a symbiotic relationship between Rhododendron fortunei Lindl. and an ERM fungus (Oidiodendron maius var. maius strain Om19), quantified seedling growth and N uptake, and compared transcriptome profiling between colonized and uncolonized roots using RNA-Seq. The Om19 colonization induced 16,892 genes that were differentially expressed in plant roots, of which 14,364 were upregulated and 2,528 were downregulated. These genes included those homologous to ATP-binding cassette transporters, calcium/calmodulin-dependent kinases, and symbiosis receptor-like kinases. N metabolism was particularly active in Om19-colonized roots, and 51 genes were upregulated, such as nitrate transporters, nitrate reductase, nitrite reductase, ammonium transporters, glutamine synthetase, and glutamate synthase. Transcriptome analysis also identified a series of genes involving endocytosis, Fc-gamma R-mediated phagocytosis, glycerophospholipid metabolism, and Gonadotropin-releasing hormone (GnRH) signal pathway that have not been reported previously. Their roles in the symbiosis require further investigation. The Om19 colonization significantly increased N uptake and seedling growth. Total N content and dry weight of colonized seedlings were 36.6 and 46.6% greater than control seedlings. This is the first transcriptome analysis of a species from the family Ericaceae colonized by an ERM fungus. The findings from this study will shed light on the mechanisms underlying symbiotic relationships of ericaceous species with ERM fungi and the symbiosis-resultant N uptake and plant growth.

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Inoculation of cranberry (Vaccinium macrocarpon) with the ericoid mycorrhizal fungus Rhizoscyphus ericae increases nitrate influx

Cited by 36 publications

References 86 publications

A New Oidiodendron maius Strain Isolated from Rhododendron fortunei and its Effects on Nitrogen Uptake and Plant Growth

A New Oidiodendron maius Strain Isolated from Rhododendron fortunei and its Effects on Nitrogen Uptake and Plant Growth

Effects of Salinity Induced by Ammonium Sulfate Fertilizer on Root and Shoot Growth of Highbush Blueberry

Differential Gene Expression in Rhododendron fortunei Roots Colonized by an Ericoid Mycorrhizal Fungus and Increased Nitrogen Absorption and Plant Growth

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