Inoculating exogenous bacterium Brevibacillus laterosporus ZR-11 at maturity stage accelerates composting maturation by regulating physicochemical parameters and indigenous bacterial community succession

Abstract

In this experiment, Brevibacillus laterosporus ZR-11, a bio-control strain with resistance to Fusarium wilt in cucumber, was inoculated in the mature manure prepared for maturing composting; conventional detection and Illumina Miseq high-throughput sequencing were adopted to investigate the changes of physicochemical factors and microbial communities in the composting process; correlation analysis was also conducted, with the purpose of providing some reference for improving the production technology of biological manure and upgrading the quality of products. According to experimental results, in the maturing period, bacterial community structures underwent substantial evolutions in both the inoculated group and the non-inoculated group Microbial diversity analysis revealed that Firmicutes, Proteobacteria, and Actinobacteria were dominant bacterial phyla in both the inoculated group and the non-inoculated group in different period, but their relative abundances were higher in the former. Through analyzing physicochemical indicators, it was found that ZR-11 could increase the temperature of maturing composting pile, accelerate compost maturation, and enhance the abundance of bacterial communities in the pile. The results of this study provided some reference for improving the maturing ING composting production technology of biological manure and upgrading the quality of products.

Brevibacillus laterosporus: A Probiotic with Important Applications in Crop and Animal Production

“…However, due to its capacity for enhancing intestinal barrier function, regulating immunity, and inhibiting the proliferation of harmful bacteria, B. laterosporus has gradually been employed as a variety of animal feed additives in the breeding industry, demonstrating significant potential within the realm of animal production. Additionally, B. laterosporus facilitated the accelerated maturation of compost by regulating physicochemical parameters and orchestrating bacterial community succession, thereby providing valuable insights for the efficient utilization of manure resources and the integration of cultivation practices with sustainable development [ 76 ].…”