Inoculated microbial consortia perform better than single strains in living soil: A meta-analysis

Liu, Xipeng; Mei, Siyu; Salles, Joana Falcão

doi:10.1016/j.apsoil.2023.105011

Cited by 25 publications

(18 citation statements)

References 62 publications

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“…We showed that inoculation of plants with microbial consortia is more effective than singlestrain in improving plant traits, supporting previous metadata studies [21,22]. A possible explanation for a larger effect of consortium than single strain inoculation can be due to the variable and synergistic plant growth-promoting activities that co-inoculation of more than one strain can offer to plants in comparison with single-strain inoculation.…”

Section: Biological Factors Influencing the Effect Sizesupporting

confidence: 89%

“…However, manipulation of plant microbiomes often does not result in the enhancement of plant traits, and the widespread application of this approach is limited by various biological and experimental factors that affect its efficiency [20]. Regarding biological factors, previous research has shown the advantage of multiple strains over single strain inoculation [21][22][23]. However, we still have a limited understanding of whether the efficiency of inoculation differs between a wide range of plant models and phenotypic traits, as well as applied microbial phyla.…”

Section: Introductionmentioning

confidence: 99%

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Biological and experimental factors that define the effectiveness of microbial inoculation on plant traits: a meta-analysis

Azarbad,

Junker

2024

Preprint

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Bacterial and fungal microbiomes associated with plants can significantly affect the host phenotype. Inoculating plants with one or multiple bacterial and fungal species can affect specific plant traits, which is exploited in attempts to increase plant performance and stress tolerance by microbiome engineering. Currently, we lack a comprehensive synthesis on the generality of these effects related to different biological (e.g., plant models, plant traits, and microbial taxa) and experimental factors. In a meta-analysis, we showed that the plant trait under consideration and the microbial taxa used to inoculate plants significantly influenced the strength of the effect size. In a methodological context, experiments under sterilized conditions and short-term periods resulted in larger positive effects on plant traits than those of unsterilized and long-term experiments. Based on our results, we propose a comprehensive checklist as a reference for future research to standardize the design, implementation, and reporting of microbial inoculation studies. We recommend that future studies should exploit the full range of the precision-realism continuum involving (short-term) lab experiments with sterilized plants and single inoculants but also and more often (long-term) field or greenhouse experiments with naturally occurring microbial communities associated with the plants and inoculated consortia including both bacteria and fungi.

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Section: Biological Factors Influencing the Effect Sizesupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Biological and experimental factors that define the effectiveness of microbial inoculation on plant traits: a meta-analysis

Azarbad,

Junker

2024

Preprint

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“…After decades of research on plant growth-promoting bacteria (PGPB) and the development of microbial-based products to support plant health (25,26), the effectiveness of these products in the field remains largely uncertain (21)(22)(23)(24). Inoculants have recently incorporated microbial consortia to improve outcomes, but formulations lack ecological validation (20,25,26). Additionally, the study of microbial interactions and their impact on community functions are generally neglected.…”

Section: Discussionmentioning

confidence: 99%

“…The use of microbial products in agriculture is a promising approach to aid plant health while reducing the use of chemical pesticides and fertilizers (19). Notably, the use of microbial consortia allowed increased performance in laboratory settings, compared to single strains (20). However, scaling to field applications is still a limiting step (21)(22)(23)(24) probably due to the deficient incorporation of microbial ecology approaches in the development of multistrain products (25,26).…”

Section: Introductionmentioning

confidence: 99%

Harnessing Emergent Properties of Microbial Consortia: Assembly of the Xilonen SynCom

Gastélum,

Gómez-Gil,

Olmedo-Álvarez

et al. 2024

Preprint

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Synthetic communities (SynComs) are valuable tools for addressing fundamental questions in microbial ecology regarding community assembly. They could also potentially aid in successfully manipulating microbial communities for clinical, biotechnological, and agricultural applications. SynCom design is complicated since interactions between microbes cannot be predicted based on their individual properties. Here, we aimed to assemble a higher-order SynCom from seed-endophytic bacteria isolated from native maize landraces. We screened co-cultures that included strains from theBacilliclass, and theBurkholderiaandPseudomonasgenera since these taxa have been previously shown to be important for the fertility of native maize landraces. We developed a combinatorial, bottom-up strategy aimed at the detection of a complex colony architecture as an emergent collective property. Using this simplified approach, we assembled a SynCom composed ofBacillus pumilusNME155,Burkholderia contaminansXM7 andPseudomonassp. GW6. The strains exhibited positive and negative interactions when evaluated in pairs, but their higher-order assembly results in a complex colony architecture, which is considered a proxy of biofilm formation. This SynCom was namedXilonenafter the Aztec goddess of young maize and fertility. TheXilonenSynCom will aid in studying the molecular and ecological basis mediating maize fertility.

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“…A recent metanalysis showed that inoculant diversity correlates significantly and positively with inoculum effect (Liu et al, 2023). One explanation could be that more species in a consortium increase its multifunctionality and stability (Jiménez et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Challenging the impact of consortium diversity on bioaugmentation efficiency and native bacterial community structure in a freshly PAH-contaminated soil

Nieto,

Festa,

Colman

et al. 2024

Preprint

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Polycyclic aromatic hydrocarbons (PAHs) are priority pollutants. We studied the effect of 20 bioaugmentation with three allochthonous bacterial consortia with increasing diversity, SC AMBk, SC1 21 and SC4, in the structure and functionality of an acutely PAH-contaminated soil microbiome. The PAH 22 supplementation increased the resource availability and the inocula were able to: efficiently degrade 23 the PAHs supplemented after 15 days of incubation, become temporary established, and modify the 24 number of total interactions with soil residents. Sphingobium and Burkholderia, both member of 25 inoculants, were the major contributors to KO linked to degradation and to differentially abundant 26 genera in inoculated microcosms, indicating their competitiveness in the soil. Bioaugmentation 27 efficiency relayed on them, while further degradation, could be carried out by native microorganism. 28 This is the one of the first works which applied three inocula, designed from naturally occurring 29 bacteria and study their effect on the soil native community through the ANCOM-BC. We revealed that 30 when a resource that can be use by the inoculant is added to the soil, it is not necessary a high-diversity 31 inoculant to interact with native community and establish itself. This result has implications in the 32 design of microbiome engineering for bioremediation processes

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Inoculated microbial consortia perform better than single strains in living soil: A meta-analysis

Cited by 25 publications

References 62 publications

Biological and experimental factors that define the effectiveness of microbial inoculation on plant traits: a meta-analysis

Biological and experimental factors that define the effectiveness of microbial inoculation on plant traits: a meta-analysis

Harnessing Emergent Properties of Microbial Consortia: Assembly of the Xilonen SynCom

Challenging the impact of consortium diversity on bioaugmentation efficiency and native bacterial community structure in a freshly PAH-contaminated soil

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