Innovative hydrolysis of corn stover biowaste by modified magnetite laccase immobilized nanoparticles

Gou, Zechang; Ling, Nyuk; Zhang, Wenqi; Lei, Zhipeng; Su, Yingjie; Sun, Chuang; Wang, Gang; Chen, Huan; Zhang, Sitong; Chen, Guang; Sun, Yansong

doi:10.1016/j.envres.2020.109829

Cited by 33 publications

(12 citation statements)

References 35 publications

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“…At the end of the 10th cycle, HNTs-M-chitosan (1%)-GTA- Laccase retained 60% of the initial activity. This result suggests exceptional reusability for the immobilized laccase, representing a significant improvement upon recently reported nano-supports for laccase immobilization [ 29 , 31 , 42 , 43 ]. Thus, the biocatalytic performance of the HNTs-M-chitosan (1%)-GTA- Laccase is excellent, resulting in a wide array of potential applications.…”

Section: Resultssupporting

confidence: 51%

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

et al. 2020

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A surface-engineered nano-support for enzyme laccase-immobilization was designed by grafting the surface of halloysite nanotubes (HNTs) with Fe3O4 nanoparticles and chitosan. Herein, HNTs were magnetized (HNTs-M) by a cost-effective reduction-precipitation method. The synthesized HNTs-M were grafted with 0.25%, 0.5%, 1%, and 2% chitosan (HNTs-M-chitosan), respectively. Synthesized HNTs-M-chitosan (0.25%), HNTs-M-chitosan (0.5%), HNTs-M-chitosan (1%) and HNTs-M-chitosan (2%) were linked with glutaraldehyde (GTA) for laccase immobilization. Among these formulations, HNTs-M-chitosan (1%) exhibited the highest laccase immobilization with 95.13% activity recovery and 100.12 mg/g of laccase loading. The optimized material was characterized thoroughly by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), X-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The immobilized laccase (HNTs-M-chitosan (1%)-GTA-Laccase) exhibited higher pH, temperature, and storage stabilities. The HNTs-M-chitosan (1%)-GTA-Laccase possesses excellent reusability capabilities. At the end of 10 cycles of the reusability experiment, HNTs-M-chitosan (1%)-GTA-Laccase retained 59.88% of its initial activity. The immobilized laccase was utilized for redox-mediated degradation of sulfamethoxazole (SMX), resulting in 41%, 59%, and 62% degradation of SMX in the presence of 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), guaiacol (GUA), and syringaldehyde (SA), respectively. Repeated SMX degradation (57.10% after the sixth cycle) confirmed the potential of HNTs-M-chitosan (1%)-GTA-Laccase for environmental pollutant degradation. Thus, we successfully designed chitosan-based, rapidly separable super-magnetic nanotubes for efficacious enhancement of laccase biocatalysis, which can be applied as nano-supports for other enzymes.

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Section: Resultssupporting

confidence: 51%

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

et al. 2020

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“…The recovery of fermentable sugars in the nano systems was observed to be slightly higher in comparison to the control experiments ( Fig. 7 ) and this can be ascribed to increased enzyme activities under the nanobiocatalyst conditions [ [50] , [51] , [52] , [53] , [54] ]. In a related study, Ban and Paul [ 25 ], reported an increase in intracellular β-glucosidase (BGL) activity up to 28 % with 5 mM ZnO nanoparticle process inclusion.…”

Section: Resultsmentioning

confidence: 89%

Impact of nanoparticle inclusion on bioethanol production process kinetic and inhibitor profile

Sanusi

Suinyuy

Kana

2021

Biotechnology Reports

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“…Desorption by a pH-trigger and a second bioprocess for cellulose hydrolysis of corn stover was done to avoid laccase inhibition. This new immobilization perspective described by Gou et al [64] also studied various laccase concentrations and incubation times to improve immobilization efficiency by adsorption. The best results were obtained for 30 min of incubation with 2.5 mg mL −1 of protein that retained activity of 87.4% with 85.3% of adsorbed protein.…”

Section: Immobilization Of Lignin-degrading Enzymesmentioning

confidence: 99%

“…It is essential to note that the optimization step is fundamental for immobilized enzyme efficiency, catalytic activity, stability, and recycling ability. The studies that previously determined the optimal immobilization parameters have usually shown better yields and efficiencies in enzyme immobilization [12,37,47,52,56,64,65].…”

Section: Key Factors Of Magnetic Enzyme Immobilizationmentioning

confidence: 99%

Magnetic Nanomaterials as Biocatalyst Carriers for Biomass Processing: Immobilization Strategies, Reusability, and Applications

2021

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Environmental concerns, along with oil shortages, have increased industrial interest in biomass conversion to produce biofuels and other valuable chemicals. A green option in biomass processing is the use of enzymes, such as cellulases, hemicellulases, and ligninolytic (laccase and peroxidases), which have outstanding specificity toward their substrates and can be reused if immobilized onto magnetic nanocarriers. Numerous studies report the biocatalysts’ performance after covalent binding or adsorption on differently functionalized magnetic nanoparticles (MNPs). Functionalization strategies of MNPs include silica-based surfaces obtained through a sol–gel process, graphene oxide-based nanocomposites, polymer-coated surfaces, grafting polymer brushes, and others, which have been emphasized in this review of the immobilization and co-immobilization of enzymes used for biomass conversion. Careful analysis of the parameters affecting the performance of enzyme immobilization for new hybrid matrices has enabled us to achieve wider tolerance to thermal or chemical stress by these biosystems during saccharification. Additionally, it has enabled the application of immobilized laccase to remove toxic organic compounds from lignin, among other recent advances addressed here related to the use of reusable magnetic carriers for bioderived chemical manufacturing.

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Innovative hydrolysis of corn stover biowaste by modified magnetite laccase immobilized nanoparticles

Cited by 33 publications

References 35 publications

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

Impact of nanoparticle inclusion on bioethanol production process kinetic and inhibitor profile

Magnetic Nanomaterials as Biocatalyst Carriers for Biomass Processing: Immobilization Strategies, Reusability, and Applications

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