Innovative gold-free carbon nanotube/chitosan-based competitive immunosensor for determination of HIV-related p24 capsid protein in serum

Giannetto, Marco; Costantini, Monica; Mattarozzi, Monica; Careri, Maria

doi:10.1039/c7ra07245g

Cited by 11 publications

(12 citation statements)

References 25 publications

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“…HIV protein 24 (p24), which is a component of the HIV capsid, is often the target molecule for detection by many biosensors [ 86 , 87 , 88 , 89 , 90 ]. However, the low sensitivity of detection of many current biosensors is the main limitation for using p24 as a target.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…However, the low sensitivity of detection of many current biosensors is the main limitation for using p24 as a target. Electrochemical immunosensors have overcome this limitation by fabricating biosensors using nanomaterials to increase sensitivity [ 86 , 87 , 88 , 89 ]. Fang et al [ 87 ] fabricated an electrochemical biosensor that is based on a sandwich immunoreaction for the detection of p24 antigen.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

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Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

2021

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The last few decades have been plagued by viral outbreaks that present some of the biggest challenges to public safety. The current coronavirus (COVID-19) disease pandemic has exponentiated these concerns. Increased research on diagnostic tools is currently being implemented in order to assist with rapid identification of the virus, as mass diagnosis and containment is the best way to prevent the outbreak of the virus. Accordingly, there is a growing urgency to establish a point-of-care device for the rapid detection of coronavirus to prevent subsequent spread. This device needs to be sensitive, selective, and exhibit rapid diagnostic capabilities. Electrochemical biosensors have demonstrated these traits and, hence, serve as promising candidates for the detection of viruses. This review summarizes the designs and features of electrochemical biosensors developed for some past and current pandemic or epidemic viruses, including influenza, HIV, Ebola, and Zika. Alongside the design, this review also discusses the detection principles, fabrication techniques, and applications of the biosensors. Finally, research and perspective of biosensors as potential detection tools for the rapid identification of SARS-CoV-2 is discussed.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

2021

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“…The immunodevice was employed on throw-away gold-free SWCNT-functionalized screen-printed electrodes. The competitory sensor depends upon immobilizing the target protein on the electrode surface by a chitosan/glutaraldehyde crosslinking system, capable of ensuring, under moderate settings, a strong immobilization and a good exhibition of p24 to interact with a mouse anti-p24 IgG1 [ 55 ].…”

Section: Application Of Carbon Nanomaterials For Viral Diagnosismentioning

confidence: 99%

Application of carbon nanomaterials in human virus detection

Ehtesabi

2020

Journal of Science: Advanced Materials and Devices

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Human-pathogenic viruses are still a chief reason for illness and death on the globe, as epitomized by the COVID-19 pandemic instigated by a coronavirus in 2020. Multiple novel sensors have been invented because diseases must be detected and diagnosed as early as possible, and recognition methods have to be carried out with minimal invasivity. Sensors have been particularly developed focusing on miniaturization by the use of nanomaterials for fabricating nanosensors. The nano-sized nature of nanomaterials and their exclusive optical, electronical, magnetical, and mechanical attributes can enhance patient care through the use of sensors with minimal invasivity and extreme sensitivity. Amongst the nanomaterials utilized for fabricating nano-sensors, carbon-based nanomaterials are promising as these sensors respond better to signals in various sensing settings. This review provides an overview of the recent developments in carbon nanomaterial-based biosensors for viral recognition based on the biomarkers that arise from the infection, the nucleic acids from the viruses, and the entire virus. The role of carbon nanomaterials is highlighted by the improvement of sensor and recognition functionality. The Dengue virus, Ebola virus, Hepatits virus, human immunodeficiency virus (HIV), influenza virus, Zika virus and Adenovirus are the virus types reviewed to illustrate the implementation of the techniques. Finally, the drawbacks and advantages of carbon nanomaterial-based biosensors for viral recognition are identified and discussed.

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“…To detect α-fetoprotein in human serum, an immunosensor was fabricated in which the α-fetoprotein antigen was immobilized on a film of a gold nanoparticles/carbon nanotubes/chitosan nanocomplex to quantify protein levels using a competitive immunoassay format (Lin et al 2009). Giannetto et al (2017) fabricated a competitive electrochemical immunosensor to detect HIV1-related capsid protein p24 in human serum. The p24 antigen was immobilized on gold-free single-walled carbon nanotubechitosan complex for the interaction with a mouse monoclonal anti-p24, which was used for competitive immunodetection.…”

Section: Biosensorsmentioning

confidence: 99%

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

Merzendorfer

Cohen

2019

Biologically-Inspired Systems

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Chitin is a linear polysaccharide of N-acetylglucosamine, which is highly abundant in nature and mainly produced by marine crustaceans. Chitosan is obtained by hydrolytic deacetylation. Both polysaccharides are renewable resources, simply and cost-effectively extracted from waste material of fish industry, mainly crab and shrimp shells. Research over the past five decades has revealed that chitosan, in particular, possesses unique and useful characteristics such as chemical versatility, polyelectrolyte properties, gel-and film-forming ability, high adsorption capacity, antimicrobial and antioxidative properties, low toxicity, and biocompatibility and biodegradability features. A plethora of chemical chitosan derivatives have been synthesized yielding improved materials with suggested or effective applications in water treatment, biosensor engineering, agriculture, food processing and storage, textile additives, cosmetics fabrication, and in veterinary and human medicine. The number of studies in this research field has exploded particularly during the last two decades. Here, we review recent advances in utilizing chitosan and chitosan derivatives in different technical, agricultural, and biomedical fields.

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Innovative gold-free carbon nanotube/chitosan-based competitive immunosensor for determination of HIV-related p24 capsid protein in serum

Abstract: The first competitive immunosensor for the determination of HIV-related p24 capsid protein, realized and validated in undiluted human serum.

Cited by 11 publications

References 25 publications

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

Application of carbon nanomaterials in human virus detection

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

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