Innovative detection methods for aquatic algal toxins and their presence in the food chain

Vilariño, Natalia; Louzao, M. Carmen; Fraga-Corral, María; Rodríguez, Laura P.; Botana, Luís M.

doi:10.1007/s00216-013-7108-6

Cited by 38 publications

(22 citation statements)

References 128 publications

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“…Algal toxins, phycotoxins, and cyanotoxins, in turn, are produced by toxicogenic microalgae and cyanobacteria, and they can enter the marine food chain via phytoplankton and subsequently to humans by contaminated seafood. Several small molecule toxins originating from seafood are known to cause severe illnesses, such as paralytic shellfish poisoning, puffer fish poisoning, and neurotoxic shellfish poisoning, and it has been reported that seafood poisonings are increasing in frequency and new intoxications are emerging [5,6]. All the aforementioned toxins are known to cause, besides a threat to human and animal health, but also substantial economic losses in aqua- and agriculture, and many of them fall under national and international regulations.…”

Section: Origin and Occurrence Of Small Moleculesmentioning

confidence: 99%

Optical Biosensors for Label-Free Detection of Small Molecules

Peltomaa

Glahn-Martínez

Benito‐Peña

et al. 2018

Sensors

168

111

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Label-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. In this review, we aim to introduce some of the latest developments in the field of label-free optical biosensors with the focus on applications with novel innovations to overcome the challenges related to small molecule detection. Optical label-free methods with different transduction schemes, including evanescent wave and optical fiber sensors, surface plasmon resonance, surface-enhanced Raman spectroscopy, and interferometry, using various biorecognition elements, such as antibodies, aptamers, enzymes, and bioinspired molecularly imprinted polymers, are reviewed.

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Section: Origin and Occurrence Of Small Moleculesmentioning

confidence: 99%

Optical Biosensors for Label-Free Detection of Small Molecules

Peltomaa

Glahn-Martínez

Benito‐Peña

et al. 2018

Sensors

168

111

View full text Add to dashboard Cite

show abstract

“…This technique is compatible with simple extraction protocols for marine biotoxins and is sensitive enough to detect these toxins below the regulated limit in shellfish samples as well as in algae and seawater. The high degree of automation, the potential to create portable devices and the ease-of-use make SPR-based immunosensors a potential tool for toxin detection [100,101]. Feltis et al [102] developed the first hand-held and field-deployable SPR biosensor for detection of security sensitive proteotoxins.…”

Section: Label-free Technologiesmentioning

confidence: 99%

Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins

Pöhlmann

Elßner

2020

Toxins

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Biological toxins are a heterogeneous group of high molecular as well as low molecular weight toxins produced by living organisms. Due to their physical and logistical properties, biological toxins are very attractive to terrorists for use in acts of bioterrorism. Therefore, among the group of biological toxins, several are categorized as security relevant, e.g., botulinum neurotoxins, staphylococcal enterotoxins, abrin, ricin or saxitoxin. Additionally, several security sensitive toxins also play a major role in natural food poisoning outbreaks. For a prompt response to a potential bioterrorist attack using biological toxins, first responders need reliable, easy-to-use and highly sensitive methodologies for on-site detection of the causative agent. Therefore, the aim of this review is to present on-site immunoassay platforms for multiplex detection of biological toxins. Furthermore, we introduce several commercially available detection technologies specialized for mobile or on-site identification of security sensitive toxins.

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“…Traditionally, these toxins have been detected in seafood using laborious biological and animal reference methods. The release of the European Union Directive 86/609 for animal protection to ensure progress away from animal experimentation to scientifically acceptable validated non-animal procedures started the scientific exploration for alternative methods for these animal bioassays [90,91]. Biosensor technologies are analytical tools for the recognition and measurement of a target through its association with a biological component and physiochemical detector.…”

Section: Biosensorsmentioning

confidence: 99%

Exploring Marine Resources for Bioactive Compounds

et al. 2014

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Biodiversity in the seas is only partly explored, although marine organisms are excellent sources for many industrial products. Through close co-operation between industrial and academic partners, it is possible to successfully collect, isolate and classify marine organisms, such as bacteria, fungi, micro- and macroalgae, cyanobacteria, and marine invertebrates from the oceans and seas globally. Extracts and purified compounds of these organisms can be studied for several therapeutically and industrially significant biological activities, including anticancer, anti-inflammatory, antiviral, antibacterial, and anticoagulant activities by applying a wide variety of screening tools, as well as for ion channel/receptor modulation and plant growth regulation. Chromatographic isolation of bioactive compounds will be followed by structural determination. Sustainable cultivation methods for promising organisms and biotechnological processes for selected compounds can be developed, as well as biosensors for monitoring the target compounds. The (semi)synthetic modification of marine-based bioactive compounds produces their new derivatives, structural analogs and mimetics that could serve as hit or lead compounds and be used to expand compound libraries based on marine natural products. The research innovations can be targeted for industrial product development in order to improve the growth and productivity of marine biotechnology. Marine research aims at a better understanding of environmentally conscious sourcing of marine biotechnology products and increased public awareness of marine biodiversity. Marine research is expected to offer novel marine-based lead compounds for industries and strengthen their product portfolios related to pharmaceutical, nutraceutical, cosmetic, agrochemical, food processing, material and biosensor applications.

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Innovative detection methods for aquatic algal toxins and their presence in the food chain

Cited by 38 publications

References 128 publications

Optical Biosensors for Label-Free Detection of Small Molecules

Optical Biosensors for Label-Free Detection of Small Molecules

Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins

Exploring Marine Resources for Bioactive Compounds

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