Innovative approach for urease inhibition by Ficus carica extract–fabricated silver nanoparticles: An in vitro study

Borase, Hemant P.; Salunkhe, Rahul B.; Suryawanshi, Rahul K.; Salunke, Bipinchandra K.; Wagh, Nilesh S.; Patil, Satish V.

doi:10.1002/bab.1341

Cited by 23 publications

(7 citation statements)

References 20 publications

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“…2C), which facilitated the stability of AgNPs. These patterns demonstrated that the AgNPs synthesized from wheat straw were face-centered cubic (FCC) structures, which coincides with previous studies (Borase et al 2015;Dhand et al 2016).…”

Section: Characterization Of Agnps Tem and Zeta Potential Analysissupporting

confidence: 91%

Biosynthesis of Silver Nanoparticles using Wheat Straw Biomass under Light Radiation and their Antibacterial Activity

Ma¹,

Li²,

Xu³

et al. 2016

BioResources

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Silver nanoparticles (AgNPs) were synthesized with wheat straw biomass at room temperature using light irradiation. The reaction conditions were optimized, including the light intensity, biomass concentration, NaCl addition, and reaction time. The silver nanoparticles fabricated at the optimum conditions (light intensity 60,000 lx, biomass concentration 2 mg/mL, and reaction time 90 min) were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and zeta potential. The TEM results showed that the silver nanoparticles were mostly spherical, with an average diameter of 17.2 nm. The zeta potential of AgNPs reached -21.6 mV. The XRD spectra showed that the AgNPs were highly crystalline, with four characteristic peaks. The FTIR of nanoparticles implied that alcohols and proteins may have a vital role in the formation and stability of AgNPs. The silver nanoparticles synthesized by wheat straw biomass revealed antimicrobial activity against Escherichia coli and Bacillus subtilis strains.

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Section: Characterization Of Agnps Tem and Zeta Potential Analysissupporting

confidence: 91%

Biosynthesis of Silver Nanoparticles using Wheat Straw Biomass under Light Radiation and their Antibacterial Activity

Ma¹,

Li²,

Xu³

et al. 2016

BioResources

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“…Usually, the activity of encapsulated enzymes decreased by a factor of 6-7 [1,6,[10][11][12][13][14][15]. Close results to our experimental data presented in this study on encapsulated urease were obtained in [16][17][18][19]. Authors, using the enzyme dextranase, obtained encapsulated enzymes with a catalytic activity equal to 80% of the activity of the free enzyme (the capsules were formed from calcium alginate with the inclusion of silica).…”

Section: Issn 2224-5308supporting

confidence: 79%

Development of a Biosensor of Urea With the Application of Polymer Technologies for Blood and Urine Analysis

Saltanat¹,

Maria²,

Аппазов³

et al. 2018

Series of Biological and Medical

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Based on polymeric nanotechnologies, enzyme sensors and microreactors have been developedin the way, that they can determine urea in liquids. The technology of manufacturing an enzymatic biosensor does not differ significantly from the known technology of manufacturing microcapsules with an enzyme by the laer-by-laer method. This allows us, when constructing a biosensor, to use the information obtained on encapsulated enzymes by other authors. It is shown that urea biosensor is able to work for a long time (up to 2 months) without significant loss of enzyme activity. Polymer technology for manufacturing sensors is less laborious and expensive compared to other similar technologies. We propose to develop biosensor devices – urea analyzers with polymer enzyme chips for express diagnostics of biological fluids (blood, urine). One of the significant results of this work from our point of view is two factors. The first factor is the optimization of the conditions for the production of a functionally active enzyme immobilized in a polyelectrolyte coating, when the enzyme after the immobilization procedure shows an activity comparable to that of a freshly prepared free enzyme. Such a result will allow reducing the cost of enzymes when creating a sensitive layer of the developed urea analyzer. And the second factor is that the polymer coating with the enzyme is able to work not only as an enzyme electrode, but also as an enzyme microreactor, without decreasing the rate of signal registration after passing the catalytic urease-urea reaction.

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“…Ag-NPs have been obtained with sizes between 50 and 250 nm using Carica papaya leaf extract; 53 with average sizes 23±12, 32±24 and 19±22 nm using Illicium verum (star anise) seed extracts, 54 and average size 73 nm using an aqueous extract of Tephrosia tinctoria. 55 Regarding spherical shape homogeneity, our synthesis method was compared with that of other works where spherical Ag-NPs are synthesized, such as in the case of other green synthesis methods that employ leaf extracts of Saraca indica, 56 Ficus carica, 57 and Cocos nucifera. 58 The chemical and physical characterization of the Ag-NP samples suggests that the Acacia extract is capable of not only reducing the silver ions but also capping the Ag-NPs and stabilizing their surface.…”

Section: Discussion Synthesis and Characterization Of Ag-npsmentioning

confidence: 99%

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using Acacia rigidula as a reducing and capping agent

Escárcega‐González¹,

Garza-Cervantes²,

Vázquez-Rodríguez³

et al. 2018

IJN

135

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IntroductionOne of the main issues in the medical field and clinical practice is the development of novel and effective treatments against infections caused by antibiotic-resistant bacteria. One avenue that has been approached to develop effective antimicrobials is the use of silver nanoparticles (Ag-NPs), since they have been found to exhibit an efficient and wide spectrum of antimicrobial properties. Among the main drawbacks of using Ag-NPs are their potential cytotoxicity against eukaryotic cells and the latent environmental toxicity of their synthesis methods. Therefore, diverse green synthesis methods, which involve the use of environmentally friendly plant extracts as reductive and capping agents, have become attractive to synthesize Ag-NPs that exhibit antimicrobial effects against resistant bacteria at concentrations below toxicity thresholds for eukaryotic cells.PurposeIn this study, we report a green one-pot synthesis method that uses Acacia rigidula extract as a reducing and capping agent, to produce Ag-NPs with applications as therapeutic agents to treat infections in vivo.Materials and methodsThe Ag-NPs were characterized using transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction, energy-dispersive spectroscopy, ultraviolet–visible, and Fourier transform infrared.ResultsWe show that Ag-NPs are spherical with a narrow size distribution. The Ag-NPs show antimicrobial activities in vitro against Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and a clinical multidrug-resistant strain of P. aeruginosa) and Gram-positive (Bacillus subtilis) bacteria. Moreover, antimicrobial effects of the Ag-NPs, against a resistant P. aeruginosa clinical strain, were tested in a murine skin infection model. The results demonstrate that the Ag-NPs reported in this work are capable of eradicating pathogenic resistant bacteria in an infection in vivo. In addition, skin, liver, and kidney damage profiles were monitored in the murine infection model, and the results demonstrate that Ag-NPs can be used safely as therapeutic agents in animal models.ConclusionTogether, these results suggest the potential use of Ag-NPs, synthesized by green chemistry methods, as therapeutic agents against infections caused by resistant and nonresistant strains.

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Innovative approach for urease inhibition by Ficus carica extract–fabricated silver nanoparticles: An in vitro study

Cited by 23 publications

References 20 publications

Biosynthesis of Silver Nanoparticles using Wheat Straw Biomass under Light Radiation and their Antibacterial Activity

Biosynthesis of Silver Nanoparticles using Wheat Straw Biomass under Light Radiation and their Antibacterial Activity

Development of a Biosensor of Urea With the Application of Polymer Technologies for Blood and Urine Analysis

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

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