Innovations in Crustacean Processing: Bioproduction of Chitin and Its Derivatives

Manuel, Heather

doi:10.1002/9781119117193.ch6

Cited by 2 publications

(7 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 Results represent the mean of three determinations (n = 3) ± standard deviation. 2 Results represent the mean of at least two determinations (n = 2) ± standard deviation and are reported on a dry weight basis (db). 3 Results represent the mean of 2 determinations (n = 2) ± standard deviation.…”

Section: Characterization Of Raw Materials (Crab Processing Discards)mentioning

confidence: 99%

“…± standard deviation and are reported on a dry weight basis (db). 2 Results represent one determination due to limited sample size available. Reported on a dry weight basis (db).…”

Section: Pigmented Protein Powdermentioning

confidence: 99%

“…1 Results represent the mean of two determinations (n = 2) ± standard deviation. 2 Result calculated as the difference between total Kjeldahl nitrogen and Lowry protein nitrogen. 3 Values in the same row with different letters are significantly different (p < 0.05) according to Tukey's test.…”

Section: Total Nitrogen Contentmentioning

confidence: 99%

“…1 Results represent one XRD scan (n = 1) per chitin sample. 2 Amorphous diffraction at 2θ. 3 Maximum intensity of the (110) peak at around 2θ.…”

Section: Color Characteristicsmentioning

confidence: 99%

“…In Atlantic Canada, snow crab ( Chionoecetes opilio ) is the most important commercial species for the fishery and the rural economy, with an export value of ~CAD 1.3 billion in 2021 and annual landings of 76,828 tonnes [ 1 ]. Snow crab is primarily processed as Individually Quick Frozen (IQF) cooked sections, which generates 25–30% (~20,000 mt/year) waste comprised of carapace (cephalothorax shells), viscera and hepatopancreas, hemolymph, residual meat, and gills [ 2 ]. Crustacean processing discards contain valuable products including proteins, lipids, astaxanthin, organic acids, essential amino acids, chitin, and calcium [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods

Burke

Kerton

2023

Marine Drugs

View full text Add to dashboard Cite

Green extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conducting as close to a 100% green extraction as possible, and developing simple processes to facilitate implementation into processing plants without the need for complicated and expensive equipment. Three crab bio-products were obtained: pigmented vegetable oil, pigmented protein powder, and chitin. Carotenoid extractions were performed using vegetable oils (corn, canola, and sunflower oils), giving between 24.85% and 37.93% astaxanthin recovery. Citric acid was used to demineralize the remaining material and afforded a pigmented protein powder. Three different proteases were used to deproteinate and isolate chitin in yields between 17.06% and 19.15%. The chitin was still highly colored and therefore decolorization was attempted using hydrogen peroxide. Characterization studies were conducted on each of the crab bio-products isolated including powder X-ray diffraction analysis on the chitin (80.18% crystallinity index, CI, achieved using green methods). Overall, three valuable bio-products could be obtained but further research is needed to obtain pigment-free chitin in an environmentally friendly manner.

show abstract

Section: Characterization Of Raw Materials (Crab Processing Discards)mentioning

confidence: 99%

“…± standard deviation and are reported on a dry weight basis (db). 2 Results represent one determination due to limited sample size available. Reported on a dry weight basis (db).…”

Section: Pigmented Protein Powdermentioning

confidence: 99%

Section: Total Nitrogen Contentmentioning

confidence: 99%

“…1 Results represent one XRD scan (n = 1) per chitin sample. 2 Amorphous diffraction at 2θ. 3 Maximum intensity of the (110) peak at around 2θ.…”

Section: Color Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods

Burke

Kerton

2023

Marine Drugs

View full text Add to dashboard Cite

show abstract

Mechanoenzymatic Breakdown of Chitinous Material to N‐Acetylglucosamine: The Benefits of a Solventless Environment

et al. 2019

View full text Add to dashboard Cite

Chitin is not only the most abundant nitrogen‐containing biopolymer on the planet, but also a renewable feedstock that is often treated as a waste. Current chemical methods to break down chitin typically employ harsh conditions, large volumes of solvent, and generate a mixture of products. Although enzymatic methods have been reported, they require a harsh chemical pretreatment of the chitinous substrate and rely on dilute solution conditions that are remote from the natural environment of microbial chitinase enzymes, which typically consists of surfaces exposed to air and moisture. We report an innovative and efficient mechanoenzymatic method to hydrolyze chitin to the N‐acetylglucosamine monomer by using chitinases under the recently developed reactive aging (RAging) methodology, based on repeating cycles of brief ball‐milling followed by aging, in the absence of bulk solvent. Our results demonstrate that the activity of chitinases increases several times by switching from traditional solution‐based conditions of enzymatic catalysis to solventless RAging, which operates on moist solid substrates. Importantly, RAging is also highly efficient for the production of N‐acetylglucosamine directly from shrimp and crab shell biomass without any other processing except for a gentle wash with aqueous acetic acid.

show abstract

Innovations in Crustacean Processing: Bioproduction of Chitin and Its Derivatives

Cited by 2 publications

References 74 publications

Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods

Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods

Mechanoenzymatic Breakdown of Chitinous Material to N‐Acetylglucosamine: The Benefits of a Solventless Environment

Contact Info

Product

Resources

About