Innervation of vas deferens and accessory male genital glands in the water buffalo (Bubalus bubalis)

Mirabella, Nicola; Squillacioti, Caterina; Varricchio, Ettore; Genovese, A.; Paino, G.

doi:10.1016/s0093-691x(02)01260-8

Cited by 12 publications

(11 citation statements)

References 54 publications

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“…A colocalization of NPY with DBH has also been described in the man (Tainio et al, 1994), hamster, (Schindelmeiser et al, 1989), bull, (Rose et al, 1992), cat (Gürtler, 2001) and pig (Kaleczyc et al, 1997(Kaleczyc et al, , 1999. For this reason NPY seems to be a modulator of adrenergic neurotransmission, playing a role in the ejaculation and sperm transport (LamanoCarvalho et al, 1986;Mirabella et al, 2003;) and regulation of blood flow (Tainio et al, 1994). The NPY-containing nerves are also distributed intimately with the epithelial cells of the monkey ejaculatory duct which have few ultrastructural features indicating a positive secretory activity (Yokoyama, 1989).…”

Section: Resultsmentioning

confidence: 93%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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Alpacas (Vicugna pacos) are domesticated camelids indigenous to south America and recently also bred in Europe and Italy for their high quality wool. There is little data available regarding the innervation of the male reproductive tract of this species. In the present study, the distribution of protein gene product 9.5 (PGP 9.5), neuropeptide Y (NPY), tyrosine hydroxilase (TH), calcitonin gene related peptide (CGRP) and substance P (SP) was analyzed in the epididymis by using immunohistochemical methods. Specimens of the caput, corpus and cauda epididymis were fixed in Bouin's fluid and processed for immunohistochemistry analysis with primary antibodies against PGP 9.5, NPY, TH, CGRP and SP. Immunopositivity to PGP 9.5 and TH and NPY was observed in nerve fibre bundles and in single nerve fibres contained into the peritubular connective tissue. Many TH and NPY immunopositive cells were found to innervate blood vessels. Rare CGRP and SP immunopositive nerves were observed. Several PGP 9,5 and NPY immunopositive epithelial cells were observed in the caput epididymis. The results of the present study suggest a role for the innervations in modulate reproductive functions in the alpaca epididymis.

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Section: Resultsmentioning

confidence: 93%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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“…Immunoreactivity to SOM was found in a few nerve fibres supplying the human seminal vesicle and prostate (Gu et al, 1983;Chapple et al, 1991;Crowe et al, 1991;Tainio, 1995). Mirabella et al (2003) revealed SOM-IR nerve fibres innervating the prostate in the water buffalo. In this species non-vascular SOM-positive axons contain neither VIP nor TH, thus these fibres probably belong to a subpopulation of non-adrenergic nerves distinct from those containing NOS, VIP and NPY.…”

Section: Discussionmentioning

confidence: 91%

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Klimczuk¹,

Kaleczyc²,

Franke-Radowiecka³

et al. 2005

Vet. Med. - Czech

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Our previous immunohistochemical investigations revealed three major populations of nerve fibres supplying the porcine accessory genital glands (AGG) including noradrenergic, non-noradrenergic putative cholinergic and sensory nerve terminals (Kaleczyc et al., 1997). However, it is still unclear whether the non-noradrenergic nerve fibres are cholinergic in nature. The knowledge of the population of cholinergic nerve fibres in mammalian AGG based upon vesicular acetylcholine transporter (VAChT) immunohistochemistry is very limited. Therefore, the aim of the present investigation was to disclose the occurrence and colocalization patterns of VAChT, dopamineâ-hydroxylase (DβH) and some neuropeptides including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and somatostatin (SOM) within nerve fibres supplying the porcine AGG. Double-immunohistochemical labellings showed that VAChT-positive nerve terminals were non-adrenergic (DâH-negative), however, many of them contained immunoreactivities to VIP, NPY and/or SOM. The coexistence patterns of these biologically active substances in nerve fibres supplying particular glands are similar but the density of cholinergic innervation varies between the organs. The innervation of the seminal vesicle and prostatic body is better developed than that of the disseminated part of the prostate and bulbourethral glands. The majority of cholinergic nerve fibres associated with blood vessels supplying the glands contain VIP and NPY and, to a lesser degree, SOM. The possible function and origin of the cholinergic nerve fibre population are discussed.

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“…The ovine prostate contained VIP-IR nerve fibres in a frequency comparable to the one found in the SV and BU but the abundance of NPY-IR nerve fibres was very low. Previously, numerous VIPand NPY-containing nerve fibres were found in the human ASG (Gu et al, 1983;Adrian et al, 1984;Higgins and Gosling, 1989;Lange and Unger, 1990;Chapple et al, 1991;Tainio, 1995;Hedlund et al, 1997;Dixon et al, 2000) and in the ASG of many other animal species (Alm et al, 1977(Alm et al, , 1980Stjernquist et al, 1983;Lamano-Carvalho et al, 1986;Moss et al, 1987;Yuri, 1990;Properzi et al, 1992;Iravani and Zar, 1994;Pinho et al, 1994;Ottesen et al, 1995;Domeneghini, 1997, 1998;Chow et al, 1997;Kaleczyc et al, 1999;Czaja et al, 2000;Majewski et al, 2001;Mirabella et al, 2003). The results of the previously mentioned studies can be summarised as follows: in all species the vast majority of VIP-IR nerve fibres were localised in the mucosal layer of the SV, BU and prostate, whereas only sparse VIP-IR nerve fibres were found within the smooth muscle layer.…”

Section: Discussionmentioning

confidence: 99%

“…The general opinion is that the development and function of the ASG are influenced both by the endocrine and the nervous system. The application of histochemical and immunohistochemical techniques has revealed autonomic nerve fibres (noradrenergic and cholinergic) Acta Veterinaria Hungarica 54, 2006 in all (or specific) ASG of man (Baumgarten et al, 1968;Dunzendorfer et al, 1976;Vaalasti and Hervonen, 1980;Gu et al, 1983;Higgins and Gosling, 1989;Chapple et al, 1991;Hedlund et al, 1997;Dixon et al, 2000), rat (Owman and Sjöstrand, 1965;Sjöstrand, 1965;Vaalasti and Hervonen, 1979;Moss et al, 1987;Yuri, 1990), hamster (Chow et al, 1997), guinea pig (Owman and Sjöstrand, 1965;Sjöstrand, 1965;Al-Zuchair et al, 1975;Lamano-Carvalho et al, 1986;Sjöstrand et al, 1998), rabbit, dog, cat (Owman and Sjöstrand, 1965;Sjöstrand, 1965), pig (Kaleczyc et al, 1999;Czaja et al, 2000;Majewski et al, 2001) and even in those of the water buffalo (Mirabella et al, 2003). It is known that nerve fibres may contain substances of peptide character, which act as neurotransmitters and/or neuromodulators.…”

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confidence: 99%

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

Arciszewski¹,

Wąsowicz

2006

Acta Veterinaria Hungarica

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Immunohistochemistry was applied to determine the distribution patterns of nerve fibres containing tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and vesicular acetylcholine transporter (VAChT) in the prostate, seminal vesicle (SV) and bulbourethral glands (BU) of male sheep. In all organs studied, cholinergic innervation was more developed than noradrenergic innervation. Numerous VAChTimmunoreactive (IR) nerve fibres were found in the muscular layer and mucosa of the SV and BU as well as in the prostate. A similar abundance of noradrenergic nerve fibres (showing immunoreactivity both to TH and DβH) was also found in both layers of the SV and BU (but not in the prostate). In the prostate a moderate density of VIP-IR nerve fibres was present but only very scarce NPY-IR nerve fibres were shown. All the studied accessory sexual glands (ASG) of male sheep contained VIP-IR nerve fibres in a similar frequency. Double immunohistochemistry revealed that the vast majority of noradrenergic nerve fibres also contained NPY. None of the noradrenergic nerve fibres showed the presence of VAChT or VIP. The possible functional significance of these findings is discussed.Key words: Autonomic innervation, neuropeptides, immunohistochemistry, accessory sexual glands, genital tract, sheep Secretions of the accessory sexual glands (ASG) contribute to the semen plasma responsible for the maintenance of a favourable environment for spermatozoa. It is generally accepted that dysfunctions of the prostate, seminal vesicles (SV) and bulbourethral glands (BU) lead to low semen quality, making the semen unsuitable for artificial insemination (AI). This is of particular importance in ruminants in which AI is routinely used. The general opinion is that the development and function of the ASG are influenced both by the endocrine and the nervous system. The application of histochemical and immunohistochemical techniques has revealed autonomic nerve fibres (noradrenergic and cholinergic)

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Innervation of vas deferens and accessory male genital glands in the water buffalo (Bubalus bubalis)

Cited by 12 publications

References 54 publications

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

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