One of the manifestations of skeletal muscle plasticity is its atrophy, as an
adaptive response to catabolic stimuli. They can occur during forced
exhausting physical activity. Injection of local anesthetics,
glucocorticosteroids, etc., is widely used to correct such pathological
manifestations. The purpose of the study was to reveal the skeletal muscle
morphofunctional characteristics of experimental animals after forced
physical activity under conditions of subcutaneous and combined methods of
administration of allogeneic biomaterial (BMA). The model of anaerobic
physical exercise was forced swimming of male rats with a load of 10% of
body weight. After the swimming test, the animals were divided into four
groups. In the first (experimental) group (n=10), the BMA suspension was
administered only subcutaneously. In the second (experimental) group (n=10),
BMA suspension was injected in combination, i.e., into the muscles of the
limbs and subcutaneously. In the control groups, saline solution was
administered using similar methods. After tissue collection on days 5 and
21, morphological, physiological, and statistical studies were carried out.
The use of BMA contributed to an increase in load tolerance, and accelerated
restoration of muscle tissue, hypertrophy, and hyperplasia. There was a
decrease in inflammation, and restoration of microcirculation and
ultrastructure of muscle fibers: contractile elements, energy balance of
cells, and proliferative activation of the nuclear apparatus. Implantation
of BMA promoted inhibition of fibrosis, reduction in the number of necrotic
muscle fibers, and chemoattraction of macrophages. The greatest
effectiveness determined was with the combined administration of the
biomaterial. BMA has an actoprotective effect.