Innervation of amphibian reproductive system. Histological and ultrastructural studies

Cisint, Susana; Crespo, Claudia A.; Medina, Marcela F.; Villagra, Lucrecia Iruzubieta; Fernández, Silvia N.; Ramos, Inés

doi:10.1016/j.autneu.2014.05.004

Cited by 3 publications

(4 citation statements)

References 39 publications

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“…Our results suggest that sympathetic innervation would be the branch of the autonomic nervous system responsible for the increase in secretory activity, taking into account that incubations performed with acetylcholine do not affect the endocrine activity of the gonad. In agreement with these observations, data reported in mammalian ovaries (Gerendai et al, 2005;Ricu et al, 2008;Sporrong et al, 1991) birds (Atoji et al, 2000) and amphibians (Cisint et al, 2014) revealed that the gonad is predominantly innervated by the sympathetic division of the autonomic nervous system. The results presented above show, for the first time in the amphibian ovary, an increase in the secretion of gonadal steroids in response to nerve stimulation of the gonad, and suggest that catecholamines could act synergistically with gonadotrophins.…”

Section: Discussionsupporting

confidence: 85%

“…Previous results from our laboratory indicate that catecholamines would be the neurotransmitters released at nerve terminals (Cisint et al, 2014); therefore, the effect of adrenaline and noradrenaline on the secretory function of the ovary was analyzed under in vitro conditions. The results obtained indicated that, out of the two catecholamines tested, only adrenaline was effective in inducing the secretion of E 2 and P 4 .…”

Section: Discussionmentioning

confidence: 99%

“…The effect of nerve stimulation and positive immunostaining to TH enzyme that intervenes in the synthesis of catecholamines and neuropeptide Y (NPY) in the nerve fibers that innervate the gonad of the species under study (Cisint et al, 2014) allows us to consider the possibility that both catecholamines and neuropeptide are involved in the increased endocrine activity of the ovary. This hypothesis would be supported by research carried out on rat ovaries in which the presence of NPY in the nerve fibers supplying the gonad was demonstrated (Jørgensen et al, 1991; Kaleczyc et al, 1995; Lara, McDonald, Ahmed, et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Considering that in the nerve fibers that supply the gonad the presence of tyrosine hydroxylase (TH), a key enzyme in the biosynthesis of catecholamines, has been demonstrated (Cisint et al, 2014), and that catecholamines could be the neurotransmitters released when electrical stimuli are applied, the influence of adrenaline and noradrenaline on gonadal steroid secretion was analyzed.…”

Section: Action Of Adrenergic Drugsmentioning

confidence: 99%

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Effect of nervous stimulation on ovarian steroid secretion in amphibians

et al. 2020

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In the present study, the effect of nerve stimulation on the secretory activity of the ovary of adult females was analyzed for the first time in amphibians. Results revealed that in Rhinella arenarum the stimulation of nerves that supply the gonad induced an increase in estradiol and progesterone secretion, this response showing differences during the reproductive cycle of the species. During the postreproductive period, an increase in estradiol secretion was observed while, in the reproductive period, progesterone secretion increased. Our results suggest that the sympathetic division of the autonomic nervous system would be responsible for this increase, taking into account that, under our experimental conditions, acetylcholine did not affect the endocrine activity of the gonad, while adrenaline (epinephrine) was effective in inducing steroid secretion an effect that could be due to interaction with β receptors. On the other hand, our data show that the association of adrenaline with follicle-stimulating hormone increased estradiol secretion during the postreproductive period, while the association of catecholamine with LH or hCG increased progesterone secretion during the reproductive period. Our results would suggest that nerve stimulation, mediated by the release of adrenaline, would act synergistically with gonadotrophins to stimulate steroid secretion.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Action Of Adrenergic Drugsmentioning

confidence: 99%

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Effect of nervous stimulation on ovarian steroid secretion in amphibians

et al. 2020

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Effects of nerve stimulation on amphibian oviductal activity

Cisint

Fernández

Crespo

et al. 2018

Micron

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A Follicle Rupture Assay Reveals an Essential Role for Follicular Adrenergic Signaling in Drosophila Ovulation

Deady

Sun

2015

PLoS Genet

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Ovulation is essential for the propagation of the species and involves a proteolytic degradation of the follicle wall for the release of the fertilizable oocyte. However, the precise mechanisms for regulating these proteolytic events are largely unknown. Work from our lab and others have shown that there are several parallels between Drosophila and mammalian ovulation at both the cellular and molecular levels. During ovulation in Drosophila, posterior follicle cells surrounding a mature oocyte are selectively degraded and the residual follicle cells remain in the ovary to form a corpus luteum after follicle rupture. Like in mammals, this rupturing process also depends on matrix metalloproteinase 2 (Mmp2) activity localized at the posterior end of mature follicles, where oocytes exit. In the present study, we show that Mmp2 activity is regulated by the octopaminergic signaling in mature follicle cells. Exogenous octopamine (OA; equivalent to norepinephrine, NE) is sufficient to induce follicle rupture when isolated mature follicles are cultured ex vivo, in the absence of the oviduct or ovarian muscle sheath. Knocking down the alpha-like adrenergic receptor Oamb (Octoampine receptor in mushroom bodies) in mature follicle cells prevents OA-induced follicle rupture ex vivo and ovulation in vivo. We also show that follicular OA-Oamb signaling induces Mmp2 enzymatic activation but not Mmp2 protein expression, likely via intracellular Ca2+ as the second messenger. Our work develops a novel ex vivo follicle rupture assay and demonstrates the role for follicular adrenergic signaling in Mmp2 activation and ovulation in Drosophila, which is likely conserved in other species.

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Innervation of amphibian reproductive system. Histological and ultrastructural studies

Cited by 3 publications

References 39 publications

Effect of nervous stimulation on ovarian steroid secretion in amphibians

Effect of nervous stimulation on ovarian steroid secretion in amphibians

Effects of nerve stimulation on amphibian oviductal activity

A Follicle Rupture Assay Reveals an Essential Role for Follicular Adrenergic Signaling in Drosophila Ovulation

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