Inner filter effect in fluorescence spectroscopy: As a problem and as a solution

Crosslinking of Streptavidin–Biotinylated Bovine Serum Albumin Studied with Fluorescence Correlation Spectroscopy

“…Since the concentration of the sample is very low and the absorption maximum (550 nm) of the excitation wavelength (~510 nm) differs by about 40 nm, the inner filter effect will be negligible. [28][29] The time required for measuring FCS per sample was 5 min and the temperature was 20 AE 3 C.…”

Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy

“…A control study ( Figures S5/S6, SI) using a Trp solution confirmed that the variance in this long wavelength region was not due to instrumental or measurement factors. It should be noted this emission is very weak, typically 2-3% of the main band for unstressed samples, and also very sensitive to Inner Filter Effects (IFE) [41]. However, for the stressed samples this long wavelength signal increased significantly ( Figure S4, SI) to more than 20% for the most mechanically stressed samples.…”

Fluorescence-Based Sensing of Pesticides Using Supramolecular Chemistry

“…Fluorescence detection can occur through a variety of mechanisms, with changes in the fluorescence signal of the system acting as the transducing element. A variety of mechanisms of fluorescence detection have been reviewed in other publications (Bissell et al, 2005;Kumar Panigrahi and Kumar Mishra, 2019;Cao et al, 2020;Wu et al, 2020), and the interested reader is directed towards these references for more information. In general, changes in the fluorescence signal upon introduction of pesticides are most often "turn-off" changes, resulting from pesticide-induced fluorescence quenching.…”