Inner filter effect-based red-shift and fluorescence dual-sensor platforms with sulfur quantum dots for detection and bioimaging of alkaline phosphatase

Ning, Keke; Fu, Y.; Wu, Jianzhong; Sun, Yujie; Liu, Ke; Kang, Yong; Liu, Jiaxin; Wu, Yuan; Liang, Jiangong

doi:10.1039/d2ay01658c

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…The IFE phenomenon refers to the absorption of the excitation and/or emission light of a fluorophore using an appropriate absorber, which results in the fluorescence quenching of the fluorophore due to the spectral overlap between the absorption (excitation) and/or emission bands of the fluorophore with the absorption band of the absorber. [53][54][55][56] In this work, as shown in Fig. 3B, the photoluminescence (PL) of the developed sensor is dynamically quenched in the pH range of 4.5 to 1.5 as a result of the relative overlap between the different absorption spectra of RCAs and the PL spectrum of UCNPs in the spectral region of 510-560 nm.…”

Section: Recommended Sensing Strategy For H Pylori Infection Diagnosismentioning

confidence: 95%

A smart nanopaper sensor for optical diagnosis ofHelicobacter pyloriinfection

Adib,

Sharifi,

Kiani

et al. 2023

Mater. Adv.

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Section: Recommended Sensing Strategy For H Pylori Infection Diagnosismentioning

confidence: 95%

A smart nanopaper sensor for optical diagnosis ofHelicobacter pyloriinfection

Adib,

Sharifi,

Kiani

et al. 2023

Mater. Adv.

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“…In contrast, fluorescence-sensing systems have drawn extensive attention from researchers due to their advantages such as simple operation, less sample consumption and real-time detection. At present, inorganic quantum dots, metal–organic frameworks, coordination polymers and traditional organic dyes have been successfully used in the development of ALP fluorescent sensors [ 16 , 17 , 18 , 19 , 20 , 21 ]. However, the current design of sensing systems still has many defects, such as the complex synthesis and modification process of fluorophores, the high toxicity of inorganic quantum dots and the poor solubility of traditional organic dyes in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Construction of Aggregation-Induced Emission Molecule–MnO2 Composite Nanoprobe and Its Application in Alkaline Phosphatase Detection

Cui

Zhao

2023

Nanomaterials

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Alkaline phosphatase (ALP) is among the most studied enzymes by far, playing an important role in the metabolism of organisms and the regulation of protein activity. Herein, a label-free composite nanoprobe is constructed by combining inorganic nanomaterials and aggregation-induced emission (AIE) molecule to achieve highly sensitive and selective detection of ALP. Negatively charged 9,10-bis [2-(6-sulfonatopropoxyl) naphthylethenyl] anthracene (BSNVA) molecule is synthesized, which has the AIE performance and can be assembled on the surface of amino–SiO2 nanoparticles through electrostatic interaction for fluorescence enhancement. MnO2 nanosheets are rich in negative charges, enabling them to be wrapped on the surface of the amino–SiO2 nanosphere to shield the positive charge on its surface, making it impossible for BSNVA to accumulate on the surface and then weakening the bio-fluorescence of the system. Furthermore, with catalyzed substrates induced by ALP, generating ascorbic acid and the redox reaction between ascorbic acid and MnO2, the nanoprobe helps in realizing the high-sensitivity detection of ALP with a detection limit of 0.38 mU/mL. The proposed strategy requires no complex cleaning and modification processes and can overcome the quenching effect caused by the aggregation of traditional organic dyes, proving to be a simple, low-cost and “turn-on” fluorescent detection method for ALP.

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Specializing Carbon Nanozyme Active Sites for Sensitive Alkaline Phosphatase Activity Metal‐Free Detection

Hsieh,

Yeh,

Wang

et al. 2023

Chemistry An Asian Journal

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As biological enzymes regulate metabolic processes, alkaline phosphatase (ALP) is a critical diagnostic indicator associated with many diseases. To accurately measure the enzyme activity, nanozymactic materials can offer sensitive strategies for ALP detection. However, nanozymes often lack specific target binding sites, and the presence of common co‐components, e. g., metal ions, may cause false‐positive or false‐negative results in enzyme activity determination. Herein, we developed a colorimetric assay for ALP detection using metal‐free nanozymatic carbon dots (CDs). The ALP hydrolysis of pyrophosphate ions (PPi) to phosphate ions (Pi) induces a “turn‐on” response based on the nanozyme activity. This PPi‐induced inhibition mechanism is extensively studied via the Michaelis‐Menten model, revealing that PPi acts as a noncompetitive inhibitor for CDs at a binding site distinct from the common nanozyme active site. With superior responses to ALP substrates, a highly sensitive and selective method is established for sensing ALP activity with a linear range of 0.010–0.200 U/L and a detection limit of 0.009 U/L. This finding explores the recognition and binding behavior of nanozymes, allowing for precise and reliable measurements even in complex samples, and represents a significant breakthrough for nanozyme‐based assays in biological analysis.

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Inner filter effect-based red-shift and fluorescence dual-sensor platforms with sulfur quantum dots for detection and bioimaging of alkaline phosphatase

Abstract: Alkaline phosphatase (ALP), one of vital biomarkers in several diseases, plays a role in indicating disease presence or severity in early diagnosis. Here, a simple H2O2 assisted top-down method was...

Cited by 4 publications

References 36 publications

A smart nanopaper sensor for optical diagnosis ofHelicobacter pyloriinfection

A smart nanopaper sensor for optical diagnosis ofHelicobacter pyloriinfection

Construction of Aggregation-Induced Emission Molecule–MnO2 Composite Nanoprobe and Its Application in Alkaline Phosphatase Detection

Specializing Carbon Nanozyme Active Sites for Sensitive Alkaline Phosphatase Activity Metal‐Free Detection

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