Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip

Vassey, Matthew; Ma, Le; Kämmerling, Lisa; Mbadugha, Chidimma; Trindade, Gustavo F.; Figueredo, Grazziela P.; Pappalardo, Francesco; Hutchinson, Jason; Markus, R. A.; Rajani, Seema; Hu, Qin; Winkler, David A.; Irvine, Derek J.; Hague, Richard; Ghaemmaghami, Amir M.; Wildman, Ricky; Alexander, Morgan R.

doi:10.1016/j.matt.2023.01.002

Cited by 10 publications

(8 citation statements)

References 42 publications

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“…Confined spaces or printed structures can lead to cell alignment or induce changes within cells leading for example to stem cell differentiation into certain cell types. [86,195,[203][204][205][206][207] For a detailed overview and analysis of these effects on single cells, we kindly refer to a recent review on this topic by Link et al [208] Recently, the investigation of single cells in dynamic environments has become possible by employing printable materials, whose properties can be changed on demand upon external stimuli. One of the first examples of such a material was presented by Hippler et al in 2020 (see Figure 7B).…”

Section: Cell Scaffoldsmentioning

confidence: 99%

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Recent Advances in Multi‐Photon 3D Laser Printing: Active Materials and Applications

Mainik,

Spiegel,

Blasco

2023

Advanced Materials

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Since the pioneering work of Kawata and colleagues in 1997, multi‐photon 3D laser printing, also known as direct laser writing, has made significant advancements in a wide range of fields. Moreover, the development and commercialization of photocurable inks for this technique have expanded rapidly. One of the current trends is the transition from static to active printable materials, often referred to as 4D microprinting, which enables a new degree of control in the printed systems. This review focuses on four primary application areas: microrobotics, optics and photonics, microfluidics, and life sciences, highlighting recent progress and the crucial role of active materials, including liquid crystalline elastomers, hydrogels, shape memory polymers, and composites, among others. It also addresses ongoing challenges and provides insights into the future prospects in the different fields.

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Section: Cell Scaffoldsmentioning

confidence: 99%

Section: Life Sciencesmentioning

confidence: 99%

Recent Advances in Multi‐Photon 3D Laser Printing: Active Materials and Applications

Mainik,

Spiegel,

Blasco

2023

Advanced Materials

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“…While the immune‐instructive effects of topographical cues are broadly attributed to changes in macrophage mechano‐sensing machinery. [ 6 , 7 ]…”

Section: Introductionmentioning

confidence: 99%

Spatially Resolved Molecular Analysis of Host Response to Medical Device Implantation Using the 3D OrbiSIMS Highlights a Critical Role for Lipids

Suvannapruk,

Fisher,

Luckett

et al. 2024

Advanced Science

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A key goal for implanted medical devices is that they do not elicit a detrimental immune response. Macrophages play critical roles in the modulation of the host immune response and are the cells responsible for persistent inflammatory reactions to implanted biomaterials. Two novel immune‐instructive polymers that stimulate pro‐ or anti‐inflammatory responses from macrophages in vitro are investigated. These also modulate in vivo foreign body responses (FBR) when implanted subcutaneously in mice. Immunofluorescent staining of tissue abutting the polymer reveals responses consistent with pro‐ or anti‐inflammatory responses previously described for these polymers. Three Dimensional OrbiTrap Secondary Ion Mass Spectrometry (3D OrbiSIMS) analysis to spatially characterize the metabolites in the tissue surrounding the implant, providing molecular histology insight into the metabolite response in the host is applied. For the pro‐inflammatory polymer, monoacylglycerols (MG) and diacylglycerols (DG) are observed at increased intensity, while for the anti‐inflammatory coating, the number of phospholipid species detected decreased, and pyridine and pyrimidine levels are elevated. Small molecule signatures from single‐cell studies of M2 macrophages in vitro correlate with the in vivo observations, suggesting potential for prediction. Metabolite characterization by the 3D OrbiSIMS is shown to provide insight into the mechanism of bio‐instructive materials as medical devices and to inform on the FBR to biomaterials.

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“…[36,37] Expanding further the potential of such HTS platforms, combinatorial platforms incorporating more surface parameters have been developed in the form of the ChemoTopoChip, utilized for studying immunomodulatory osteoinductive biomaterials, and the ChemoArchiChip which led to discovering that macrophage attachment and phenotype can be tuned via highly specific combinations of size, geometry, and material. [51,52] These examples showcase and stress the need to look at combined influences of material chemistry and surface T. A different approach to libraries of distinct surface properties is the use of surface parameter gradients, where a parameter is gradually altered from one end of a spectrum to the other, which allows to investigate cell response to a large span of different cell-biomaterial interfaces, on the same material. Following a similar combinatorial approach and increasing complexity, orthogonal gradients have been used to investigate two parameters simultaneously, such as W/T [53] and W/S, [54] but also physicochemical-biological property combinations such as orthogonal gradients of peptides mimicking cell-cell or cellmatrix interactions into a 3D hydrogel system, to investigate the influence of these peptides on cartilage formation.…”

Section: Introductionmentioning

confidence: 99%

Double‐Orthogonal Gradient‐Based High‐Throughput Screening Platform for Studying Cell Response Toward Combined Physicochemical Biomaterial Properties

van der Boon,

Tromp,

et al. 2023

Small Science

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Implant‐associated complications arise due to non‐optimized cell–biomaterial interactions. It is well known that cells respond to their physicochemical microenvironment on 2D interfaces and 3D networks. Attempts to manipulate this interaction target surface parameters such as wettability (W), stiffness (S), and topography (T) to influence cell differentiation, adhesion, and morphology, due to induction of gene activation and protein expression. Investigating the combinatorial influence of all three mentioned parameters simultaneously remains challenging, though most realistic, since all three parameters are inherently present on a surface. Herein, a novel high‐throughput screening technology, which allows investigating the cell response of human bone‐marrow‐derived mesenchymal stem cells toward three varying biomaterial surface parameters simultaneously, is presented. The platform provides efficient screening and cell response readout to a vast amount of combined biomaterial surface properties, in a single‐cell experiment. Surface gradients of aligned wrinkle T, S, and W are orthogonally combined giving four combinatorial surfaces. The screening outcome is validated by translating interesting regions to homogeneous surfaces. Cells are found to behave similar to the screening in terms of adhesion, spreading, and vimentin expression. The technology tremendously supports the identification of optimal surface parameter combinations and potentially addressing many of the current implant‐associated complications.

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Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip

Cited by 10 publications

References 42 publications

Recent Advances in Multi‐Photon 3D Laser Printing: Active Materials and Applications

Recent Advances in Multi‐Photon 3D Laser Printing: Active Materials and Applications

Spatially Resolved Molecular Analysis of Host Response to Medical Device Implantation Using the 3D OrbiSIMS Highlights a Critical Role for Lipids

Double‐Orthogonal Gradient‐Based High‐Throughput Screening Platform for Studying Cell Response Toward Combined Physicochemical Biomaterial Properties

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