Innate factors causing differences in gene flow frequency from transgenic rice to different weedy rice biotypes

Zuo, Jianru; Zhang, Lianju; Song, Xiaoling; Dai, Weimin; Qiang, Sheng

doi:10.1002/ps.2108

Cited by 23 publications

(13 citation statements)

References 37 publications

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“…Weedy rice is often managed using flooding and/or herbicides and seldom occurs in other crops or in nonagricultural habitats. Pollen-mediated gene flow from rice to weedy rice has been reported (Chen et al, 2004;Shivrain et al, 2007;Tian et al, 2011;Zuo et al, 2011), and herbicide resistance transgenes in cultivated rice are expected to spread to weedy rice within a few seasons of contact (Gressel, 1999;Zuo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A novel 5‐enolpyruvoylshikimate‐3‐phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide

et al. 2013

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Understanding evolutionary interactions among crops and weeds can facilitate effective weed management. For example, gene flow from crops to their wild or weedy relatives can lead to rapid evolution in recipient populations. In rice (Oryza sativa), transgenic herbicide resistance is expected to spread to conspecific weedy rice (Oryza sativa f. spontanea) via hybridization.Here, we studied fitness effects of transgenic over-expression of a native 5-enolpyruvoylshikimate-3-phosphate synthase (epsps) gene developed to confer glyphosate resistance in rice. Controlling for genetic background, we examined physiological traits and field performance of crop–weed hybrid lineages that segregated for the presence or absence of this novel epsps transgene.Surprisingly, we found that transgenic F2 crop–weed hybrids produced 48–125% more seeds per plant than nontransgenic controls in monoculture- and mixed-planting designs without glyphosate application. Transgenic plants also had greater EPSPS protein levels, tryptophan concentrations, photosynthetic rates, and per cent seed germination compared with nontransgenic controls.Our findings suggest that over-expression of a native rice epsps gene can lead to fitness advantages, even without exposure to glyphosate. We hypothesize that over-expressed epsps may be useful to breeders and, if deployed, could result in fitness benefits in weedy relatives following transgene introgression.

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Section: Introductionmentioning

confidence: 99%

A novel 5‐enolpyruvoylshikimate‐3‐phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide

et al. 2013

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“…Given the important implications of these findings, more sampling and investigation of PA rice are warranted. Outcrossing rates between weedy and cultivated rice in field trials have been found to be low, between 0.03 and 0.25% and between 0 and 6.66% (Shivrain et al ., ; Zuo et al ., ). Traits such as overlap in plant height and flowering time seem to impact that rate (Zuo et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Outcrossing rates between weedy and cultivated rice in field trials have been found to be low, between 0.03 and 0.25% and between 0 and 6.66% (Shivrain et al ., ; Zuo et al ., ). Traits such as overlap in plant height and flowering time seem to impact that rate (Zuo et al ., ). PA rice acting as a potential conduit between weedy and cultivated rice helps explain the high morphological diversity in weedy rice populations in the United States (Shivrain et al ., ,b) despite the low natural hybridisation rate and the absence of wild relatives.…”

Section: Discussionmentioning

confidence: 97%

“…There is also evidence of hybridisation between BHA rice and some late flowering indica cultivars based on field trials (Gealy et al ., ), indicating that crop–weed introgression is possible. Outcrossing rates between transgenic lines of cultivated rice and weedy rice in field trials have been measured between 0 and 6.66% (Zuo et al ., ). Outcrossing rates between 0.03 and 0.25% were measured in field experiments between SH, BHA and BrH weedy rice and the herbicide‐resistant Clearfield™ cultivar (Shivrain et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Pink‐awned weedy rice (Oryza sativa): a potential conduit for gene exchange in rice agro‐ecosystems

2018

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Hybridisation between genetically distinct lineages results in increases in overall genetic diversity and is a potential mechanism for the origin and spread of adaptive alleles. Weed and crop hybridisation may result in weedy ecotypes, which have, in addition to classic weedy traits such as seed shattering and long seed dormancy, crop traits that enhance weediness, such as adaptation to field cultivation and harvest strategies. Weedy rice (Oryza sativa) hybridises with cultivated rice and, in the process, may produce new (pink-awned) weedy rice varieties. Pink-awned (PA) weedy rice plants have been observed in rice fields in Arkansas. We explored the genetic relationships between PA weedy rice, crop rice, global rice accessions and other weedy rice ecotypes present in the southern United States. Gene sequencing of 48 sequence-tagged sites (STS loci) revealed a pattern of hybridisation and gene flow between blackhull weedy rice and tropical japonica rice (O. sativa subsp. japonica) cultivars. Our data suggest that PA rice originates from hybridisation between blackhull weedy rice and tropical japonica rice. PA rice offspring segregate phenotypic traits associated with weediness in rice. This segregation could lead to adaptive allele combinations in PA rice, which could potentially move into other weedy rice types through subsequent hybridisation events.

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“…variable rates depending on flowering synchrony, environmental conditions, WR biotype and CL cultivar [12,13]. The continuous use of IMI herbicides for several seasons selects RWR individuals and may lead to the replacement of susceptible alleles by IMI resistance alleles in the WR population, that is, genetic assimilation [14].…”

mentioning

confidence: 99%

One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.)

Rosas

Bonnecarrère

Vida

2014

Electronic Journal of Biotechnology

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Background: Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience's Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice. Results: Validation was carried out by determining the analytical performance of the new method and comparing it with conventional allele-specific PCR, when genotyping sets of cultivated and weedy rice samples. The conventional technique had a specificity of 0.97 and a sensibility of 0.95, whereas for the KASP method, both parameters were 1.00. Conclusions:The new method has equal accuracy while being more informative and saving time and resources compared with conventional methods, which make it suitable for monitoring imidazolinone-resistant weedy rice in Clearfield rice fields.

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Innate factors causing differences in gene flow frequency from transgenic rice to different weedy rice biotypes

Abstract: From this study, it is concluded that the likelihood of gene flow between transgenic rice and weedy rice biotypes is primarily determined by floral synchronisation and secondarily influenced by genetic compatibility and some morphological characteristics.

Cited by 23 publications

References 37 publications

A novel 5‐enolpyruvoylshikimate‐3‐phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide

A novel 5‐enolpyruvoylshikimate‐3‐phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide

Pink‐awned weedy rice (Oryza sativa): a potential conduit for gene exchange in rice agro‐ecosystems

One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.)

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