Innate defenses of the avian egg

Réhault-Godbert, Sophie; Hincke, Maxwell T.; Guabiraba, Rodrigo; Guyot, Nicolas; Gautron, Joël

doi:10.1016/b978-0-12-818708-1.00032-4

Cited by 3 publications

(5 citation statements)

References 181 publications

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“…Additionally, avidin (AVD), unique to avian species and upregulated, was central in the network. AVD, known for its broad-spectrum antibacterial activity, is strongly stimulated by interferon α, but its role in viral infections remains unclear [69,70]. Interestingly, we found the avian-specific non-coding RNA LOC121107141 at the network's core and upregulated, suggesting its potential function in antiviral immunity.…”

Section: Plos Pathogensmentioning

confidence: 76%

Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

Liukang,

Zhao,

Tian

et al. 2024

PLoS Pathog

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Infectious bronchitis virus (IBV) is a coronavirus that infects chickens, which exhibits a broad tropism for epithelial cells, infecting the tracheal mucosal epithelium, intestinal mucosal epithelium, and renal tubular epithelial cells. Utilizing single-cell RNA sequencing (scRNA-seq), we systematically examined cells in renal, bursal, and tracheal tissues following IBV infection and identified tissue-specific molecular markers expressed in distinct cell types. We evaluated the expression of viral RNA in diverse cellular populations and subsequently ascertained that distal tubules and collecting ducts within the kidney, bursal mucosal epithelial cells, and follicle-associated epithelial cells exhibit susceptibility to IBV infection through immunofluorescence. Furthermore, our findings revealed an upregulation in the transcription of proinflammatory cytokines IL18 and IL1B in renal macrophages as well as increased expression of apoptosis-related gene STAT in distal tubules and collecting duct cells upon IBV infection leading to renal damage. Cell-to-cell communication unveiled potential interactions between diverse cell types, as well as upregulated signaling pathways and key sender-receiver cell populations after IBV infection. Integrating single-cell data from all tissues, we applied weighted gene co-expression network analysis (WGCNA) to identify gene modules that are specifically expressed in different cell populations. Based on the WGCNA results, we identified seven immune-related gene modules and determined the differential expression pattern of module genes, as well as the hub genes within these modules. Our comprehensive data provides valuable insights into the pathogenesis of IBV as well as avian antiviral immunology.

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Section: Plos Pathogensmentioning

confidence: 76%

Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

Liukang,

Zhao,

Tian

et al. 2024

PLoS Pathog

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“…Since the CAM provides a physical barrier between the weakened eggshell and the water-rich allantoic fluid, this extra-embryonic structure could also help limit water loss. Aside from its role as a physical barrier, the CAM may also participate in egg defense by its capability to stimulate molecular and cellular responses to microbial stimuli ( Hincke et al, 2019 ; Réhault-Godbert et al, 2021 ). Deposition of lipopolysaccharide (LPS, an essential component of the outer membrane of Gram-negative bacteria) on the CAM after ES opening was shown to induce a significant inflammatory response that is illustrated by the recruitment of heterophils and monocytes to the immunostimulated site, via the CAM vasculature ( Valdes et al, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

Concomitant Morphological Modifications of the Avian Eggshell, Eggshell Membranes and the Chorioallantoic Membrane During Embryonic Development

et al. 2022

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The chicken eggshell (ES) consists of 95% calcium carbonate and 3.5% organic matter, and represents the first physical barrier to protect the developing embryo, while preventing water loss. During the second half of development, calcium ions from the inner ES are progressively solubilized to support mineralization of the embryonic skeleton. This process is mediated by the chorioallantoic membrane (CAM), which is an extraembryonic structure that adheres to the eggshell membranes (ESM) lining the inner ES. The CAM surrounds the embryo and all egg contents by day 11 of incubation (Embryonic Incubation Day 11, EID11) and is fully differentiated and functionally active by day 15 of incubation (Embryonic Incubation Day 15, EID15). In this study, we explored the simultaneous morphological modifications in the ES, ESM and the CAM at EID11 and EID15 by scanning electron microscopy. We observed that the tips of the mammillary knobs of the ES remain tightly attached to the ESM fibers, while their bases become progressively eroded and then detached from the bulk ES. Concomitantly, the CAM undergoes major structural changes that include the progressive differentiation of villous cells whose villi extend to reach the ESM and the ES. These structural data are discussed with respect to the importance of ES decalcification in providing the calcium necessary for mineralization of embryo’s skeleton. In parallel, eggshell decalcification and weakening during incubation is likely to impair the ability of the ES to protect the embryo. It is assumed that the CAM could counteract this apparent weakening as an additional layer of physical, cellular and molecular barriers against environmental pressures, including pathogens, dehydration and shocks. However, such hypothesis needs to be further investigated.

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“…As a result, the spectrum of an eggshell without a cuticle would consist of pure calcite (the mineral that makes up the eggshell). The main transmission bands in the spectrum of calcite were identified at 713, 875, 1419-1424, 1031-1164, and 1799-1800 cm -1 , which are associated with carbonate groups (Dominguez-Gasca et al, 2017;Réhault-Godbert et al, 2021;Kulshreshtha et al, 2022).…”

Section: Infrared Spectroscopymentioning

confidence: 99%

“…Therefore, the intensity ratio between the main carbonate peak and the amide I peak can be used as an indicator of cuticle quality. Comparing the intensity ratio between polysaccharide and amide peaks can be useful for determining the degree of glycosylation of cuticle proteins (Rodríguez-Navarro et al, 2013;Poyatos Pertiñez et al, 2020;Réhault-Godbert et al, 2021).…”

Section: Infrared Spectroscopymentioning

confidence: 99%

“…For example, protein content affects cuticle resistance to bacterial invasion. Fourier transform infrared spectroscopy with attenuated total internal reflection (ATR-FTIR) is a characterization method that measures cuticle thickness, amount and chemical composition (Rodríguez-Navarro et al, 2013;Bain et al, 2019;Réhault-Godbert et al, 2021;Kulshreshtha et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cold atmospheric pressure air plasma jet disinfection of table eggs: Inactivation of Salmonella enterica, cuticle integrity and egg quality

Abdoli,

Khoshtaghaza,

Ghomi

et al. 2024

International Journal of Food Microbiology

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Innate defenses of the avian egg

Cited by 3 publications

References 181 publications

Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

Concomitant Morphological Modifications of the Avian Eggshell, Eggshell Membranes and the Chorioallantoic Membrane During Embryonic Development

Cold atmospheric pressure air plasma jet disinfection of table eggs: Inactivation of Salmonella enterica, cuticle integrity and egg quality

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