Innate cellular immune function of anterior kidney leucocytes in the gulf killifish,Fundulus grandis

Roszell, L.E.; Rice, Charles D.

doi:10.1006/fsim.1997.0124

Cited by 12 publications

(3 citation statements)

References 29 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was based on a study of anterior kidney leukocytes in Gulf Killifish (Roszell and Rice 1998), wherein eosinophilic granular cells and macrophages were observed to be more common and to have greater phagocytic capacity than neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Isolates of Streptococcus agalactiae from Diseased Farmed and Wild Marine Fish from the U.S. Gulf Coast, Latin America, and Thailand

Soto¹,

Wang

Wiles

et al. 2015

J Aqua Anim Hlth

View full text Add to dashboard Cite

We examined Lancefield serogroup B Streptococcus isolates recovered from diseased, cultured hybrid Striped Bass (Striped Bass Morone saxatilis × White Bass M. chrysops) and wild and cultured Gulf Killifish Fundulus grandis from coastal waters of the U.S. Gulf of Mexico (Gulf coast) and compared those isolates to strains from tilapias Oreochromis spp. reared in Mississippi, Thailand, Ecuador, and Honduras and to the original Gulf coast strain identified by Plumb et al. ( 1974 ). The isolates were subjected to phylogenetic, biochemical, and antibiotic susceptibility analyses. Genetic analysis was performed using partial sequence comparison of (1) the 16S ribosomal RNA (rRNA) gene; (2) the sipA gene, which encodes a surface immunogenic protein; (3) the cspA gene, which encodes a cell surface-associated protein; and (4) the secY gene, which encodes components of a general protein secretion pathway. Phylogenies inferred from sipA, secY, and cspA gene sequence comparisons were more discriminating than that inferred from the 16S rRNA gene sequence comparison. The U.S. Gulf coast strains showed a high degree of similarity to strains from South America and Central America and belonged to a unique group that can be distinguished from other group B streptococci. In agreement with the molecular findings, biochemical and antimicrobial resistance analyses demonstrated that the isolates recovered from the U.S. Gulf coast and Latin America were more similar to each other than to isolates from Thailand. Three laboratory challenge methods for inducing streptococcosis in Gulf Killifish were evaluated-intraperitoneal (IP) injection, immersion (IMM), and immersion plus abrasion (IMMA)-using serial dilutions of S. agalactiae isolate LADL 97-151, a representative U.S. Gulf coast strain. The dose that was lethal to 50% of test fish by 14 d postchallenge was approximately 2 CFU/fish via IP injection. In contrast, the fish that were challenged via IMM or IMMA presented cumulative mortality less than 40% by 14 d postchallenge.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of Isolates of Streptococcus agalactiae from Diseased Farmed and Wild Marine Fish from the U.S. Gulf Coast, Latin America, and Thailand

Soto¹,

Wang

Wiles

et al. 2015

J Aqua Anim Hlth

View full text Add to dashboard Cite

show abstract

“…In addition, NCC, thought to be the counterpart of mammalian natural killer cells, have also been characterized in some fish species (Dalmo et al. 1997; Roszell & Rice 1998; Evans, Leary & Jaso‐Friedmann 2001; Ruiz, Leary & Jaso‐Friedmann 2001; Taylor, Jaso‐Friedmann, Allison, Eldar & Evans 2001). NCC and an NCC receptor (NCCRP‐1) have been characterized in tilapia and catfish (Jaso‐Friedmann, Leary & Evans 1997, 2001; Evans et al.…”

Section: Innate Immunitymentioning

confidence: 99%

The eel immune system: present knowledge and the need for research

Nielsen

Esteve-Gassent

2006

Journal of Fish Diseases

View full text Add to dashboard Cite

The European eel, Anguilla anguilla, is one of the most important warm water fish species cultured in southern Europe and the Mediterranean as well as in northern countries including Germany, the Netherlands and Denmark. The Japanese eel, A. japonica, is an important cultured fish in several Asiatic countries including Japan, China and Taiwan. During recent decades, research has been performed to elucidate the immune response of these species against different pathogens (viruses, bacteria or parasites). Nevertheless, there is very limited information in terms of both cellular and humoral immune responses. This review summarizes the present knowledge relating to the eel immune system and includes new data.

show abstract

“…Anterior kidney and spleens were collected, pushed through 80 x mesh screens in Hanks balanced salt solution (166 mM NaCl, 6.5 mM KCl, 6.8 mM glucose, 0.05 mM KH 2 PO 4 , 0.07 mM Na 2 HPO 4 , pH 7.2), hereafter referred to as HBSS. Cells were centrifuged over Percoll (Sigma Chemical Company, St. Louis MO USA) gradients of 1.050 and 1.080 g ml −1 as previously described for gulf killifish, Fundulus grandis [32], a closely related species of the mummichog. This isolation procedure concentrates macrophages and granulocytes.…”

Section: Methodsmentioning

confidence: 99%

Development, characterization, and technical applications of a fish lysozyme-specific monoclonal antibody (mAb M24-2)

Marsh

Rice

2010

Comparative Immunology, Microbiology and Infectious Diseases

Self Cite

View full text Add to dashboard Cite

Lysozyme is one of several humoral and cellular factors associated with front line, innate immunity in all vertebrates. Historically, circulating lysozyme has been quantified in teleosts by measuring enzymatic activity against heat-killed Mycococcus lysodieticus using whole serum or plasma at a low pH. However, the amount of serum or plasma required for standard lysozyme activity exceeds that which can be easily acquired from small fish, thus making lysozyme a difficult endpoint to measure in limited sample volumes. Moreover, while circulating lysozyme is considered to be an indicator of proinflammatory phagocyte activity, the cellular source of this protein is not easily detected in fish. While several antibodies against lysozyme are commercially available for use in higher vertebrates, neither reacts with lysozyme in fish. In this study, a monoclonal antibody (mAb) for detecting and quantifying lysozyme was developed from mummichog, Fundulus heteroclitus, myeloid cells that also recognizes hen egg lysozyme (HEL), then tested for cross-reactivity in different species of teleosts, A single protein of ≈ 14–15 kDa mass was identified by the mAb in fish cell lysates and plasma samples, as well as denatured HEL. Total circulating lysozyme protein was compared to lysozyme activity using standard ELISA procedures and was found to correlate with enzymatic activity. Using mAb M24-2, intracellular lysozyme protein was detected in formalin-fixed and permeabilized lymphoid cells adhered to glass cover slips. Moreover, mAb M24-2 localizes lysozyme to myeloid cells. Finally, it was demonstrated that mAb M24-2 is suitable for immunohistochemistry in that lysozyme could be detected in plastic-embedded lymphoid tissues.

show abstract

Innate cellular immune function of anterior kidney leucocytes in the gulf killifish,Fundulus grandis

Cited by 12 publications

References 29 publications

Characterization of Isolates of Streptococcus agalactiae from Diseased Farmed and Wild Marine Fish from the U.S. Gulf Coast, Latin America, and Thailand

Characterization of Isolates of Streptococcus agalactiae from Diseased Farmed and Wild Marine Fish from the U.S. Gulf Coast, Latin America, and Thailand

The eel immune system: present knowledge and the need for research

Development, characterization, and technical applications of a fish lysozyme-specific monoclonal antibody (mAb M24-2)

Contact Info

Product

Resources

About