Inkjet printing of macromolecules on hydrogels to steer neural stem cell differentiation

Ilkhanizadeh, Shirin; Teixeira, Ana I.; Hermanson, Ola

doi:10.1016/j.biomaterials.2007.05.018

Cited by 221 publications

(163 citation statements)

References 33 publications

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“…The printer can be useful in analysis in many fields, including but not limited to: single cell mechanics, tissue engineering, gene transfection, biosensor micropatterning, and direct cell therapies. [1][2][3][4][5][6][7][8][9][10][16][17][18][19][20][21][22] In this example, an HP Deskjet 500 printer and HP 26 series ink cartridges were modified for bioprinting. Using this printer setup with a bioink consisting of a fibroblast cell suspension in a g-actin monomer solution, cells were printed onto glass microscope coverslips.…”

Section: Representative Resultsmentioning

confidence: 99%

“…[2][3][4][5][6][7][8] This modified printer setup can be used for applications other than cell printing. [16][17][18][19][20][21][22] Matrix proteins, such as collagen or fibronectin, can be easily printed onto substrates with this technique, which can be useful for cell patterning. For instance, collagen type I printed into line patterns will result in aligned collagen substrates that can be used for in vitro cell culture studies.…”

Section: Discussionmentioning

confidence: 99%

“…17 In addition to matrix proteins, other molecules, including growth factors, can be reliably localized on substrates for cell studies and potential therapeutic applications. 18 A major limitation of the design described in the above steps is that this printer is not capable of printing in more than one dimension. This limits the potential for use in patterned applications such as scaffold printing.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11][12][16][17][18][19][20][21][22] The future applications of this type of device are numerous, including: creation of controlled and patterned cellular microenvironments, incorporation of macromolecules into cell cytoplasm, and deposition of cells onto scaffolds and structures that do not occur naturally or efficiently.…”

Section: Discussionmentioning

confidence: 99%

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Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Owczarczak

Shuford

Wood

et al. 2012

JoVE

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Bioprinting has a wide range of applications and significance, including tissue engineering, direct cell application therapies, and biosensor microfabrication. [1][2][3][4][5][6][7][8][9][10] Recently, thermal inkjet printing has also been used for gene transfection. 8,9 The thermal inkjet printing process was shown to temporarily disrupt the cell membranes without affecting cell viability. The transient pores in the membrane can be used to introduce molecules, which would otherwise be too large to pass through the membrane, into the cell cytoplasm. 8,9,11 The application being demonstrated here is the use of thermal inkjet printing for the incorporation of fluorescently labeled g-actin monomers into cells. The advantage of using thermal ink-jet printing to inject molecules into cells is that the technique is relatively benign to cells. 8,12 Cell viability after printing has been shown to be similar to standard cell plating methods 1,8 . In addition, inkjet printing can process thousands of cells in minutes, which is much faster than manual microinjection. The pores created by printing have been shown to close within about two hours. However, there is a limit to the size of the pore created (~10 nm) with this printing technique, which limits the technique to injecting cells with small proteins and/or particles. 8,9,11 A standard HP DeskJet 500 printer was modified to allow for cell printing. 3,5,8 The cover of the printer was removed and the paper feed mechanism was bypassed using a mechanical lever. A stage was created to allow for placement of microscope slides and coverslips directly under the print head. Ink cartridges were opened, the ink was removed and they were cleaned prior to use with cells. The printing pattern was created using standard drawing software, which then controlled the printer through a simple print command. 3T3 fibroblasts were grown to confluence, trypsinized, and then resuspended into phosphate buffered saline with soluble fluorescently labeled g-actin monomers. The cell suspension was pipetted into the ink cartridge and lines of cells were printed onto glass microscope cover slips. The live cells were imaged using fluorescence microscopy and actin was found throughout the cytoplasm. Incorporation of fluorescent actin into the cell allows for imaging of short-time cytoskeletal dynamics and is useful for a wide range of applications. 13-15

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Section: Representative Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Owczarczak

Shuford

Wood

et al. 2012

JoVE

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“…[27] Ilkhanizadeh et al found that Inkjet-printed macromolecules remained biologically functional when printed on polyacrylamide-based hydrogels, and influences the differentiation of multipotent primary fetal NSCs in an efficient and wellcontrolled manner. [28] Piezoelectric-based droplet ejectors, harmless to biological samples have been used for continuous or drop-on-demand ejection of the fluid. [29] The non-contact piezoelectric based printers are in use but with few limitations because of pressure induced cell damage.…”

Section: Fig 7: Schematic Representation Of 3d Printing Technologymentioning

confidence: 99%

Computational Approaches in Tissue Engineering

Sah¹,

Sadanand²,

Pramanik³

2011

IJCA

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Scaffolds designed with intricate and controlled interior architecture represent a challenging problem for tissue engineering. Various scaffold-fabricating techniques allow the creation of complex micro-structure but with irregular pore characteristics, resulting in inappropriate & asymmetrical structures not suitable for tissue engineering applications. Computer-Aided Tissue Engineering (CATE) integrates advanced technologies from Biology, Information Science and Engineering for Tissue Engineering applications. In particulars, computer-aided design (CAD), medical image processing, computer-aided manufacturing (CAM), and solid freeform fabrication (SFF) are employed for simulation, design and manufacturing of tissue scaffolds with controlled and regular pore architecture. CATE application to the design and fabrication of scaffolds can guide to improve the biomimetic and biological features of the scaffolds. This paper aims to understand the principles behind various computer aided approaches being utilized for tissue engineering applications particularly cell-scaffold implant modeling, designing and manufacturing.

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Bioprinting, Inkjet Deposition

Saunders

Derby

2010

Encyclopedia of Industrial Biotechnology

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Inkjet printing is a versatile deposition and patterning technology that can be used for the controlled placement of picoliter volumes of fluids. The mechanisms of drop generation are reviewed and the fluid requirements for successful printing discussed. Inkjet printing has been successfully used for the deposition of biomaterials, proteins, and living cells. The response of these materials to the printing process is discussed. A number of practical applications are discussed, including biosensors, experimental cell biology, and tissue engineering or regenerative medicine.

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Inkjet printing of macromolecules on hydrogels to steer neural stem cell differentiation

Cited by 221 publications

References 33 publications

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Computational Approaches in Tissue Engineering

Bioprinting, Inkjet Deposition

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