Inking a specimen without the mess: Figure 1

Shinde, Vijay Kumar; Phelan, Colleen A; Gater, W.; Thomas, Jesse

doi:10.1136/jcp.2008.055962

Cited by 14 publications

(13 citation statements)

References 2 publications

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“…Several staining techniques are proposed in the literature [2][3][4][5][6][7][8][9][10][11]. In our laboratory, India ink has been used traditionally to mark surgical resection margins and more recently with acetone or Bouin solution to aid drying.…”

Section: Introductionmentioning

confidence: 99%

Indian ink vs tissue marking dye: a quantitative comparison of two widely used macroscopical staining tool

2010

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The evaluation of the surgical margins is a major concern in surgical pathology, and marking of surgical margins with substances such as alcian blue, Tipp-ex, artist's pigments, colored gelatin, starch, erythrocyte layers, etc. was recommended for this purpose; Indian ink and tissue marking dyes are widely used. As there is no systematic study comparing tissue marking dyes and Indian ink as the most common substances used for the purpose, this study was conducted to compare the two. Penetration into the tissue, brightness under the microscope, the spreading area of one drop of dye on tissue paper, the intensity of colors, and unit price were compared for each of the five colors of Rotring's Indian ink and Thermo-Shandon's tissue marking dyes, applied on reduction mammoplasty specimens. Rotring's Indian ink is proved to be just as effective as Thermo-Shandon's tissue marking dye and bares the majority of the characteristics of a perfect staining substance, which are easily applied, quickly fixed, durable and cheap, contain no potential contaminants, be work safe, would not smudge/stain surrounding tissues, and look bright under the microscope without obscuring the view.

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Section: Introductionmentioning

confidence: 99%

Indian ink vs tissue marking dye: a quantitative comparison of two widely used macroscopical staining tool

2010

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“…Histopathological assessment of radical or wide excision specimens requires proper orientation of the specimen along with identification of all surgical margins (1)(2)(3)(4)(5)(6)(7)(8). Several methods have been utilised for margin assessment like the marking of surgical margins with sutures and clips or applying india ink (1,2).…”

Section: Introductionmentioning

confidence: 99%

“…Several methods have been utilised for margin assessment like the marking of surgical margins with sutures and clips or applying india ink (1,2). india ink has long been used in the field of surgical pathology (1,2,5,7,9,10). However, when multiple margins are to be assessed, there has been a lack of use of varied coloured inks (1,2).…”

Section: Introductionmentioning

confidence: 99%

Inking in surgical pathology: does the method matter? a procedural analysis of a spectrum of colours

Pursnani¹,

Arora²,

Katyayani³

et al. 2016

TJPATH

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Objective: Proper identification of surgical margins along with margin status holds utmost importance in histopathology. inking margins is one way. india ink has long been used but it can ink only one margin. On other hand acrylic colours, available in variety of colours can be used for inking multiple margins along with many more advantages. The present study was undertaken to analyse acrylic colours and indian ink for inking surgical margins via three different methods for optimum results. Material and Method:Thirteen acrylic colours along with india ink were evaluated via three different methods on radical specimens of breast and colon after preliminary requisites of grossing were completed. in Method 1, coloured inks were applied to an overnight formalin fixed specimen and representative sections were taken. in Method 2, the specimen was inked and kept for overnight fixation. The specimen was sampled following day. in Method 3, the specimen was inked and kept for overnight fixation. The following day, it was re-coloured with the same colours as of the previous day and subsequently sections were taken. Coloured inks were assessed on different parameters for their performance as surgical ink and given scores.Results: acrylic shades #04, #22, #06, #01, #02 and india ink had very good to excellent score on microscopy in all three methods. Shades #64, #18, #09, #23 had poor microscopic visibility in all three methods. Shade #09 showed loss of colour and the shade #23 showed penetration into deeper tissues on microscopy. results were best with Method 3 followed by Method 1 and 2, respectively. Conclusion: acrylic colours have more advantages compared to india ink. Method 3 is recommended for inking. Few acrylic shades meet the criteria of surgical inks in all three methods.

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“…In view of these benefits of acrylic colours, we have chosen them over others for this study. [1][2][3][4][5][6] Inking of the specimens is being widely practiced in routine processing of the tumours for margin clearance in oncology service laboratories. Various authors have used it to improve the diagnostic yield and identification of margin involvement.…”

Section: Discussionmentioning

confidence: 99%

“…Acrylic colors have many advantages as compared to others in terms of cost effectiveness, availability, reproducibility of results and ease of application. [4][5][6] India ink imparts a single colour whereas acrylic colors are available in different shades of colors where it can be easily applied for identifying various surgical margins of radical specimen in cases of malignancy. [2] For routine histopathology specimens, for example in case of appendix and fallopian tube, multiple sections are taken and embedded in paraffin blocks then slides are prepared for each.…”

Section: Introductionmentioning

confidence: 99%

Surgical Inking and Double Embedding Technique for Routine Histopathology to Save Time and Effort

Nerune

Mestri

Konduru

et al. 2018

APALM

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Background: Routine histopathological specimens like appendix and fallopian tube require individual processing, block making and slide preparation. This process is not only time consuming and requires more effort but also takes up more space during archiving. The objective of the present study was to use commercially available different acrylic colors to ink specimen of appendix and fallopian tube of different patients, process them in a single cassette and preparation of single paraffin block and slide.Methods: 100 cases of appendix and fallopian tubes were taken to evaluate ink mapping technique using Fevicryl® Acrylic colours. Blocks and slides were prepared, labelling on the slides was done using the chromacodes method for identification of the specimen. These slides were then reported by the pathologist.Result: There was a 21% reduction in the amount of paraffin required for preparation of a double embedded block in comparison to two paraffin blocks by conventional method. It was also noted that there was 18% reduction in volume as compared to two conventional paraffin blocks. Conclusion:This method of using surgical inking technique not only brings down the effort and time required, but also storage space and the costs involved along with faster identification and reporting compared to the conventional method.

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Inking a specimen without the mess: Figure 1

Cited by 14 publications

References 2 publications

Indian ink vs tissue marking dye: a quantitative comparison of two widely used macroscopical staining tool

Indian ink vs tissue marking dye: a quantitative comparison of two widely used macroscopical staining tool

Inking in surgical pathology: does the method matter? a procedural analysis of a spectrum of colours

Surgical Inking and Double Embedding Technique for Routine Histopathology to Save Time and Effort

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