Injection of Cerium Oxide Nanoparticles To Treat Spinal Cord Injury In Rats

Behroozi, Zahra; Rahimi, Behnaz; Hamblin, Michael R.; Nasirinezhad, Farinaz; Janzadeh, Atousa; Ramezani, Fatemeh

doi:10.21203/rs.3.rs-547438/v1

Cited by 5 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the motor function of the left paw, it was observed that the Scaffold alone could not improve movement, but the Scaffold-CeO 2 significantly improved the left paw movement compared to the SCI and Scaffold groups, although this improvement did not reach the level of the control group. This result is consistent with the results of other studies that showed injection of cerium oxide nanoparticles has helped to improve motor function after spinal cord injury [53,54].…”

Section: Discussionsupporting

confidence: 93%

“…Kim et al also showed that CeONPs had an antioxidant effect in spinal cord injury and subsequently improved the functional recovery in rats after mild traumatic brain injury [53]. In last work our team also demonstrated the healing effect of soluble CeONPs on neuronal regeneration after SCI [54] but at the present study, the release of nanoparticles was continuously from the fabricated scaffold, and the novelty of this study lies in this issue. In the present study, we investigated the effect of a gelatinous poly (ε-caprolactone) scaffold containing CeONPs (Scaffold-CeO 2) implanted at the site of injury on nerve cell growth and pain relief in a SCI animal model.…”

mentioning

confidence: 60%

“…Protein bands were detected by enhanced chemiluminescence (ECL). The results were quantified by Image J software [54].…”

Section: Western Blottingmentioning

confidence: 99%

See 2 more Smart Citations

Study of nerve cell regeneration on nanofibers containing cerium oxide nanoparticles in a spinal cord injury model in rats

Rahimi

Behroozi

Motamed

et al. 2023

J Mater Sci: Mater Med

View full text Add to dashboard Cite

Since the CNS is unable to repair itself via neuronal regeneration in adult mammals, alternative therapies need to be found. The use of cerium oxide nanoparticles to repair nerve damage could be a promising approach for spinal cord reconstruction. In this study, we constructed a scaffold containing cerium oxide nanoparticles (Scaffold-CeO2) and investigated the rate of nerve cell regeneration in a rat model of spinal cord injury. The scaffold of gelatin and polycaprolactone was synthesized, and a gelatin solution containing cerium oxide nanoparticles was attached to the scaffold. For the animal study, 40 male Wistar rats were randomly divided into 4 groups (n = 10): (a) Control; (b) Spinal cord injury (SCI); (c) Scaffold (SCI + scaffold without CeO2 nanoparticles); (d) Scaffold-CeO2 (SCI + scaffold containing CeO2 nanoparticles). After creation of a hemisection SCI, scaffolds were placed at the site of injury in groups c and d, and after 7 weeks the rats were subjected to behavioral tests and then sacrificed for preparation of the spinal cord tissue to measure the expression of G-CSF, Tau and Mag proteins by Western blotting and Iba-1 protein by immunohistochemistry. The result of behavioral tests confirmed motor improvement and pain reduction in the Scaffold-CeO2 group compared to the SCI group. Decreased expression of Iba-1 and higher expression of Tau and Mag in the Scaffold-CeO2 group compared to the SCI group could be the result of nerve regeneration caused by the scaffold containing CeONPs as well as relief of pain symptoms. Graphical Abstract

show abstract

Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 60%

See 1 more Smart Citation

Study of nerve cell regeneration on nanofibers containing cerium oxide nanoparticles in a spinal cord injury model in rats

Rahimi

Behroozi

Motamed

et al. 2023

J Mater Sci: Mater Med

View full text Add to dashboard Cite

show abstract

“…Although all people experience pain, many patients suffered chronic pain, which is so excruciating and affects the patient's quality of life [1][2][3]. Various chemicals [4] and natural substances [5,6] and new treatment methods such as photobiomodulation therapy [7,8] have shown analgesic effects but their administration is not approved.…”

Section: Introductionmentioning

confidence: 99%

The effect of gabapentin and pregabalin administration on memory in clinical and preclinical studies: a meta-analysis and systematic review

et al. 2023

Self Cite

View full text Add to dashboard Cite

Background Today, gabapentinoids such as Gabapentin (GBP) and pregabalin (PGB) are widely used as painkillers. This may alter the function of the nervous system; hence their results may include a difference in memory and processes that end in memory formation. This study aims to conclude whether gabapentinoids can alter memory or not by reviewing and analyzing clinical and preclinical studies. Material and methods A comprehensive search was carried out in databases including PUBMED, EMBASE, SCOPUS, and Web of Science. In the included studies, memory was measured as an outcome variable in clinical or preclinical studies. Result A total of 21 articles (4 clinical, 17 preclinical) were included in the meta-analysis by STATA Software. The results showed that memory changes under the influence of GBP. Both the administrated dosage and the time of administration are important in the final results and latency time of retention. GBP administration in healthy animals increased latency time, whereas if the administration of GBP took place exactly before training, the latency time increased slightly. Short-term administration of PGB in healthy volunteers is accompanied by transient side effects on the CNS. However, the number and homogeneity of the studies were not such that a meta-analysis could be performed on them. Conclusion Clinical and preclinical studies showed that PGB administration did not confirm its improving memory effect. GBP administration in healthy animals increased latency time and improved memory. Although it depended on the time of administration.

show abstract

“…[3,4]. Traumatic SCI often lead to devastating loss of sensory and motor function, and despite therapeutic methods which have shown positive results in the research phase [5][6][7][8][9][10] so far there is no drug treatments that can restore function consistently in affected patients Although there is some spontaneous regenerative responses that occurs following SCI, this is often insufficient for significant improvement [11]. Several factors, including inappropriate immune responses, glial scar development, and lack of adequate neurotrophic support after SCI may be the cause of this lack of regenerative ability [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Sustained delivery of chABC improves functional recovery after a spine injury

et al. 2022

Self Cite

View full text Add to dashboard Cite

Introduction Chondroitinase ABC (chABC) is an enzyme could improve regeneration and thereby improving functional recovery of spinal cord injury (SCI) in rodent models. Degradation of the active enzyme and diffusion away from the lesion are the causes of using hydrogels as a scaffold to deliver the chABC into the lesion site. In this meta-analysis, we investigated the effects of chABC embedded in a scaffold or hydrogel on the functional recovery after SCI. Method Databases were searched based on keywords related to chABC and spinal cord injury (SCI). Primary and secondary screening was performed to narrow down study objectives and inclusion criteria, and finally the data were included in the meta-analysis. The standard mean difference of the score of the functional recovery that measured by Basso, Beattie, Bresnahan (BBB) test after SCI was used to analyze the results of the reported studies. Subgroup analysis was performed based on SCI model, severity of SCI, transplantation type, and the follow-up time. Quality control of articles was also specified. Results The results showed that embedding chABC within the scaffold increased significantly the efficiency of functional recovery after SCI in animal models (SMD = 1.95; 95% CI 0.71–3.2; p = 0.002) in 9 studies. SCI model, severity of SCI, injury location, transplantation type, and the follow-up time did not affect the overall results and in all cases scaffold effect could not be ignored. However, due to the small number of studies, this result is not conclusive and more studies are needed. Conclusion The results could pave the way for the use of chABC embedded in the scaffold for the treatment of SCI and show that this method of administration is superior to chABC injection alone.

show abstract

Injection of Cerium Oxide Nanoparticles To Treat Spinal Cord Injury In Rats

Cited by 5 publications

References 27 publications

Study of nerve cell regeneration on nanofibers containing cerium oxide nanoparticles in a spinal cord injury model in rats

Study of nerve cell regeneration on nanofibers containing cerium oxide nanoparticles in a spinal cord injury model in rats

The effect of gabapentin and pregabalin administration on memory in clinical and preclinical studies: a meta-analysis and systematic review

Sustained delivery of chABC improves functional recovery after a spine injury

Contact Info

Product

Resources

About