Injection of Autologous Muscle Stem Cells (Myoblasts) for the Treatment of Vocal Fold Paralysis: A Pilot Study

Halum, Stacey L.; Naidu, Moumita; Delo, Dawn; Atala, Anthony; Hingtgen, Cynthia M.

doi:10.1097/mlg.0b013e31803e8c8d

Cited by 45 publications

(61 citation statements)

References 37 publications

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“…Most importantly, MSCs can act as cellular vectors for delivery of key neurotrophic factors, thereby selectively guiding and directing neuronal regeneration. Preliminary studies support the feasibility of using MSCs for the treatment of laryngeal denervation, with viable injected MSCs detectable in the denervated hemilarynx for at least a 2‐month period, with resultant attenuation of muscle atrophy 1 …”

Section: Introductionmentioning

confidence: 83%

Optimization of Autologous Muscle Stem Cell Survival in the Denervated Hemilarynx

et al. 2008

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Section: Introductionmentioning

confidence: 83%

Optimization of Autologous Muscle Stem Cell Survival in the Denervated Hemilarynx

et al. 2008

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“…These findings suggest that tissue-engineered muscle thickness (survival) is directly dependent on innervation, and that atrophy will occur without innervation, just as atrophy occurs when innate myofibers become acutely denervated. 13,14 …”

Section: Discussionmentioning

confidence: 99%

Stem Cell–Derived Tissue–Engineered Constructs for Hemilaryngeal Reconstruction

Halum

Bijangi-Vishehsaraei

Zhang

et al. 2014

Ann Otol Rhinol Laryngol

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Objectives As an initial step toward our goal of developing a completely tissue-engineered larynx, the aim of this study was to describe and compare three strategies of creating tissue-engineered muscle-polymer constructs for hemilaryngeal reconstruction. Methods Cartilage-mimicking polymer was developed from electrospun poly(D,L-lactide-co-ε-caprolactone) (PCL). Primary muscle progenitor cell cultures were derived from syngeneic F344 rat skeletal muscle biopsies. Twenty F344 rats underwent resection of the outer hemilaryngeal cartilage with the underlying laryngeal adductor muscle. The defects were repaired with muscle stem cell–derived muscle–PCL constructs (5 animals), myotubc-derived muscle–PCL constructs (5 animals), motor end plate–expressing muscle–PCL constructs (5 animals), or PCL alone (controls; 5 animals). The outcome measures at 1 month included animal survival, muscle thickness, and innervation status as determined by electromyography and immunohistochemistry. Results All of the animals survived the 1-month implant period and had appropriate weight gain. The group that received motor end plate–expressing muscle–PCL constructs demonstrated the greatest muscle thickness and the strongest innervation, according to electromyographic activity and the percentage of motor end plates that had nerve contact. Conclusions Although all of the tissue-engineered constructs provided effective reconstruction, those that expressed motor end plates before implantation yielded muscle that was more strongly innervated and viable. This finding suggests that this novel approach may be useful in the development of a tissue-engineered laryngeal replacement.

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“…Tissue harvest and cell culture technique were performed according to Halum et al with slight modifications 12 . Muscle was obtained from the gluteus maximus and larynx of 250 g Wistar rats.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Laryngeal Muscle Stem Cell Survival and Proliferation

et al. 2008

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Injection of Autologous Muscle Stem Cells (Myoblasts) for the Treatment of Vocal Fold Paralysis: A Pilot Study

Abstract: Autologous myoblast therapy may be a future treatment for vocal fold paralysis, with current findings demonstrating myoblast survival with attenuation of TA muscle atrophy.

Cited by 45 publications

References 37 publications

Optimization of Autologous Muscle Stem Cell Survival in the Denervated Hemilarynx

Optimization of Autologous Muscle Stem Cell Survival in the Denervated Hemilarynx

Stem Cell–Derived Tissue–Engineered Constructs for Hemilaryngeal Reconstruction

Characterization of Laryngeal Muscle Stem Cell Survival and Proliferation

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