Injection molded lab-on-a-disc platform for screening of genetically modified <i>E. coli</i> using liquid–liquid extraction and surface enhanced Raman scattering

Morelli, Lidia; Serioli, Laura; Centorbi, Francesca Alessandra; Jendresen, Christian Bille; Matteucci, M.; Ilchenko, Oleksii; Demarchi, Danilo; Nielsen, Alex Toftgaard; Zór, Kinga; Boisen, Anja

doi:10.1039/c7lc01217a

Cited by 42 publications

(41 citation statements)

References 37 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous works, we combined LLE and the here exploited gold-capped silicon nanopillar substrates for extraction and SERS detection of pHCA. 32,33 Besides excluding Tyr and salts, measuring the target analyte in the organic phase (DCM) provided the optimal working conditions for metal-capped nanopillar substrates, since it was demonstrated that wetting the substrate with an organic solvent improves the leaning of silicon nanopillars, 56 leading to a better SERS signal enhancement. With LLE we were able to extract both pHCA and CA from aqueous samples.…”

Section: Lle and Sers For Extraction And Detection Of Phca And Camentioning

confidence: 99%

“…30 In spite of the advantages of SERS-based sensing, the presence of interfering compounds and salts represents another issue for quantitative analysis in complex sample matrices, such as bacterial supernatant. 31 To address this issue, we have successfully combined SERS-based sensing with sample pretreatment techniques, such as liquid-liquid extraction (LLE) 32,33 and supported liquid membrane extraction. 34 Sample pretreatment enabled the separation of the target analyte from interfering compounds in the supernatant matrix.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Morelli

Centorbi

Ilchenko

et al. 2019

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Lle and Sers For Extraction And Detection Of Phca And Camentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Morelli

Centorbi

Ilchenko

et al. 2019

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lab-on-a-chip platforms can be categorized on the basis of their underlying liquid handling principles such as electroosmosis, pressure, peristaltics, ultrasonics, and electrowetting. Centrifugal Along with fluidic functionality, it is critically important that Lab-on-a-Disc cartridges be amenable to mass manufacturing techniques such as injection molding [78]. This work describes the initial development of this mixing structure and the primary manufacturing method, hot embossing, was selected to ensure the designs could easily be translated for manufacture by injection molding.…”

Section: Introductionmentioning

confidence: 99%

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

Burger¹,

Kinahan

Cayron

et al. 2019

Inventions

View full text Add to dashboard Cite

We present a powerful and compact batch-mode mixing and dilution technique for centrifugal microfluidic platforms. Siphon structures are designed to discretize continuous flows into a sequence of droplets of volumes as low as 100 nL. Using a passive, self-regulating 4-step mechanism, discrete volumes of two fluids are alternatingly issued into a common intermediate chamber. At its base, a capillary valve acts as a fluidic shift register; a single droplet is held in place while two or more droplets merge and pass through the capillary stop. These merged droplets are advectively mixed as they pass through the capillary valve and into the receiving chamber. Mixing is demonstrated for various combinations of liquids such as aqueous solutions as well as saline solutions and human plasma. The mixing quality is assessed on a quantitative scale by using a colorimetric method based on the mixing of potassium thiocyanate and iron(III) chloride, and in the case of human plasma using a spectroscopic method. For instance, volumes of 5 µL have been mixed in less than 20 s. Single-step dilutions up to 1:5 of plasma in a standard phosphate buffer solution are also demonstrated. This work describes the preliminary development of the mixing method which has since been integrated into a commercially available microfluidic cartridge.Inventions 2020, 5, 1 2 of 14 microfluidic systems constitute a subset which uses the rotation of the chip, which is typically of a similar geometry as a Compact Disc™ or DVD™, to enable most pumping and valving operations. These systems can be further sub-divided into 'Bioanalytical Screening CDs' and 'Lab-on-a-Disc (LoaD)'. Bioanalytical screening CDs focus on adapting optical disc drive (ODD) technology (e.g., Compact Disc, DVD) for biological detection [9]. This technology takes advantage of the high-density data storage capability as well as the favorable pricing for its main constituents, i.e., the optical pickup unit, the spindle drive, and the optical disc substrates [10][11][12][13][14].The LoaD [15][16][17][18][19][20] is typically used to automate the standard laboratory steps conducted in a biology lab to enable a fully automated sample-to-answer system. The LoaD is particularly applicable for deployment in the field (point-of-use) or at the hospital bed-side (point-of-care) as the chips can be loaded at atmospheric pressure (via pipette or syrette) without issues regarding sealing or priming. The use of centrifugation for pumping also reduces the cost and complexity of support instrumentation as just low-cost spindle motors are required rather than specialized micropumps. Similarly, inherent centrifugation of samples [21][22][23] applied to sample preparation is a particular strength.The technological precursor of these systems are the centrifugal analyzers which became popular in the 1970s and 1980s [24] for automating a very limited number of simple assay steps, on rather macroscopic sample volumes, and readout on a rotor-based instrument. Currently the main areas of application are immuno...

show abstract

“…For example, Alexei et al utilized pneumatic pumping for a multi-cycle LLE process on a centrifugal microfluidic device [19]. Also, Lidia et al performed LLE on a centrifugal microfluidic device with extraction efficiency matching that of conventional batch extraction [20].…”

Section: Introductionmentioning

confidence: 99%

Determination of Mercury(II) on A Centrifugal Microfluidic Device Using Ionic Liquid Dispersive Liquid−Liquid Microextraction

et al. 2019

View full text Add to dashboard Cite

An integrated centrifugal microfluidic device was developed to preconcentrate and detect hazardous mercury (II) in water with ionic liquid as environmentally friendly extractant. An automatically salt-controlled ionic liquid dispersive liquid–liquid microextraction on a centrifugal microfluidic device was designed, fabricated, and characterized. The entire liquid transport mixing and separation process was controlled by rotation speed, siphon valves, and capillary valves. Still frame images on the rotating device showed the process in detail, revealing the sequential steps of mixing, siphon priming, transportation between chambers, and phase separation. The preconcentration of red dye could be clearly observed with the naked eye. By combining fluorescence probe and microscopy techniques, the device was tested to determine ppb-level mercury (II) in water, and was found to exhibit good linearity and low detection limit.

show abstract

Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering

Cited by 42 publications

References 37 publications

Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

Determination of Mercury(II) on A Centrifugal Microfluidic Device Using Ionic Liquid Dispersive Liquid−Liquid Microextraction

Contact Info

Product

Resources

About