Background: People Who Inject Drugs (PWID) are at a higher risk of acquiring bloodborne infections. This study aimed to estimate the prevalence of Hepatitis C Virus (HCV) in People Who Inject Drugs (PWID) and identify correlates and risk factors using data from the Puerto Rico National HIV Behavioral System, PWID cycle 5 conducted in 2018.Methods: A total of 502 San Juan Metropolitan Statistical Area participants were recruited through Respondent Driven Sampling method. Sociodemographic, health-related, and behavioral characteristics were assessed. Detection of HCV antibodies was completed after the face-to-face survey. Descriptive and logistic regression analyses were performed using STATA 17™. Results: The prevalence of HCV was 76.5% (95%CI: 70.8%-81.4%). Adjusted multivariate logistic regression models showed that frequent speedball injectors and knowing the HCV serostatus of the last sharing partner were significantly more associated with HCV infection (ORa = 4.17; 95% CI: 1.40-12.42; ORa = 4.49; 95% CI: 1.43-14.09, respectively).Conclusions: A high prevalence of HCV infection in PWID was observed in this sample, validating the continuing call for local action for public health and prevention strategies.