Injectable Tissue-Engineered Soft Tissue for Tissue Augmentation

Rhee, Sung Mi; You, Hi-Jin; Han, Seung Kyu

doi:10.3346/jkms.2014.29.s3.s170

Cited by 21 publications

(16 citation statements)

References 25 publications

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“…Our previous clinical studies demonstrated that injection of cultured human dermal fibroblasts suspended in HA fillers was effective in the correction of wrinkles and soft-tissue augmentation during several years, and this strategy may be a suitable method for soft-tissue augmentation. 6,29,30 CONCLUSIONS Intra-and/or subdermally injected fibroblasts suspended in HA filler stay at the site of injection without dispersing to other sites. In addition, PGE1 treatment may increase the level of remaining RITC dye at the injected site of the human dermal fibroblasts.…”

Section: Discussionmentioning

confidence: 96%

Tracking and Increasing Viability of Topically Injected Fibroblasts Suspended in Hyaluronic Acid Filler

You

Namgoong²,

Rhee³

et al. 2016

Journal of Craniofacial Surgery

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A new injectable tissue-engineered soft tissue consisting of a mixture of hyaluronic acid (HA) filler and cultured human fibroblasts have been developed by the authors. To establish this method as a standard treatment, a further study was required to determine whether the injected fibroblasts could stay at the injected place or move to other sites. In addition, effective strategies were needed to increase viability of the injected fibroblasts. The purpose of this study was to track the injected fibroblasts and to determine the effect of adding prostaglandin E1 (PGE1) or vitamin C on the viability of fibroblasts.Human fibroblasts labeled with fluorescence dye were suspended in HA filler and injected into 4 sites on the back of nude mice. The injected bioimplants consisted of one of the 4 followings: HA filler without cells (HA group), fibroblasts suspended in HA filler (HA þ FB group), PGE1-supplemented fibroblasts in HA filler (HA þ FB þ PGE1 group), and vitamin C-supplemented fibroblasts in HA filler (HA þ FB þ VC group). At 4 weeks after injection, locations and intensities of the fluorescence signals were evaluated using a live imaging system.The fluorescence signals of the fibroblast-containing groups were visible only at the injected sites without dispersing to other sites. The HA þFB þ PGE1 group showed a significantly higher fluorescence signal than the HA þ FB and the HA þ FB þVC groups (P < 0.05, each). There was no statistical difference between the HA þ FB and HA þ FB þVC groups (P ¼ 0.69).The results of the current study collectively suggest that injected fibroblasts suspended in HA filler stay at the injected place without moving to other sites. In addition, PGE1 treatment may increase the remaining rhodamine B isothiocynanate dye at the injected site of the human dermal fibroblasts.F or correction of facial wrinkles or soft-tissue augmentation, a number of commercially prepared injectable fillers have been developed. 1-4 Currently, soft-tissue filler products based on hyaluronic acid (HA) are widely used, because they have a low potential for allergic reactions, require no skin testing, can be stored at room temperature, and have no risk of bovine spongiform encephalopathy unlike collagen. Although HA fillers have shown to be relatively safe and convenient to administrate, their variable degrees of resorption require repeated injections. To overcome these drawbacks, the authors created a new injectable filler consisting of a mixture of HA filler and living cultured human fibroblasts. 5,6 The results of our previous experimental and clinical studies showed that cultured human dermal fibroblasts mixed into HA filler can produce human dermal matrices with extended in vivo stability and may have a potential to be used as a long-lasting injectable soft-tissue filler. To establish this method as a standard treatment, a further study, however, is required to determine whether the fibroblasts could stay at the injected place or move to other sites through examination of the location, distribution, and long-...

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Section: Discussionmentioning

confidence: 96%

Tracking and Increasing Viability of Topically Injected Fibroblasts Suspended in Hyaluronic Acid Filler

You

Namgoong²,

Rhee³

et al. 2016

Journal of Craniofacial Surgery

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Section: Polymeric Injectable Fillersmentioning

confidence: 99%

“…In recent years, autologous fat regained interests as the source of adipose stem cells. In 2014, a permanent filler which was composed of HA and live human mesenchymal cells was reported by Rhee et al 51 It was described as injectable tissue engineered soft tissue, and had been used in clinic. In 2015, Huang et al 52 reported that human adipose stem cells (HASCs) proliferated and further differentiated into adipose tissue in HA hydrogels in vitro, which remained the potential of HA-based scaffold as adipose tissue filler.…”

Section: Representative Type D Fillermentioning

confidence: 99%

Polymeric injectable fillers for cosmetology: Current status, future trends, and regulatory perspectives

Zhao

Zhang

et al. 2019

J of Applied Polymer Sci

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Injectable fillers have become a vital part of cosmetology. A wide variety of injectable fillers are developed for either correction of facial wrinkles and folds or facial contouring. As the most popular filler products, polymeric injectable fillers represent a class of the most common implantable medical devices. This review is intended to not only present current filler products, but also share perspectives of future trends, development processes, and regulatory considerations for the next generation of fillers. First, currently approved polymeric injectable fillers are described in detail. Second, future trends of polymeric injectable fillers are discussed. Fillers based on the emerging technologies in tissue engineering and regenerative medicine with stimuli‐responsive or tunable properties, as well as device/drug combination products, are promising candidates. Third, fillers for cosmetology are different from traditionally recognized medical devices. As a result, the target population of such fillers is different from that of other implantable devices, leading to different user needs that impact their development processes. Last, product applications, regulatory requirements, device evaluation, characteristics, and difficulties for regulating filler products for minimally invasive cosmetology are reviewed. Both regulatory science and scientific supervision are needed for polymeric injectable fillers which are facing great opportunities as well as challenges. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48515.

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Section: Plastic Surgerymentioning

confidence: 99%

“…The compound is injected inside the body with a syringe and let it gels restoring the volume. This procedure started to receive criticisms by the scientific community, but yet at the moment there is still not a sufficient amount of publications investigating the properties and possible risks of the gel, concerns started to rise in particular about the long term side effects of this treatment and more specifically about HA's role in cancer control and development [52,53].Another promising use of hydrogels is bulking agents for treatment of urinary incontinence: smart injectable gels can be involved in clinical procedures where these materials can be used to tighten the urethral channel and reduce patient's incontinence. With such a simple solution it is possible to erase or at least reduce a consistent social handicap and help patients to hold a normal life [54].…”

mentioning

confidence: 99%

History and Applications of Hydrogels

Yahia¹

2015

J Biomed Sci

161

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Hydrogels have existed for more than half century, providing one of the earliest records of crosslinked hydroxyethyl methacrylate (HEMA) hydrogels. Today, hydrogels still fascinate material scientists and biomedical researchers and great strides have been made in terms of their formulations and applications. As a class of material, hydrogels are unique, they consist of a self-supporting, waterswollen three-dimensional (3D) viscoelastic network which permits the diffusion and attachment of molecules and cells. However, hydrogels have recently drawn great attention for use in a wide variety of biomedical applications such as cell therapeutics, wound healing, cartilage/bone regeneration and the sustained release of drugs. This is due to their biocompatibility and the similarity of their physical properties to natural tissue. This review aims to give an overview of the historic and the recent design concept of hydrogels and their several applications based on the old and the most recent publications in this field. Journal of Biomedical Sciences ISSN 2254-609XThis Article is Available in: www.jbiomeds.com 2 Hydrogels can be classified into two distinct categories, the natural and the synthetic hydrogels. Natural hydrogels include collagen, fibrin, hyaluronic acid, matrigel, and derivatives of natural materials such as chitosan, alginate and skill fibers. They remain the most physiological hydrogels as they are components of the extracellular matrix (ECM) in vivo. Two main drawbacks of natural hydrogels, however, make their final microstructures and properties difficult to control reproducibly between experiments. First, the fine details of their mechanical properties and their dependence on polymerization or gelation conditions are often poorly understood. Second, due to their natural origin (bovine fibrinogen, rat tail collagen… their composition may vary from one batch to another.In contrast, synthetic hydrogels such as poly (ethylene glycol) diacrylate, poly(acryl amide), poly(vinyl alcohol) are more reproducible, although their final structure can also depend on polymerization conditions in a subtle way, so that a rigorous control of the preparation protocol, including temperature and environment control, may be necessary. Generally speaking, synthetic hydrogels offer more flexibility for tuning chemical composition and mechanical properties; users can, for example vary the concentration or molecular weight of the precursor, or alter the percentage of crosslinkers. They can also be selected or tuned to be hydrolysable or biodegradable over variable periods of time.Hydrogels may be chemically stable or they may degrade and eventually disintegrate and dissolve. They are called «reversible» or «physical» gels when the networks are held together by molecular entanglements, and/or secondary forces including ionic, H-bonding or hydrophobic forces (Figure 2) [3]. Physical hydrogels are not homogeneous, since clusters of molecular entanglements, or hydrophobically-or ionically-associated domains, can create in h...

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Injectable Tissue-Engineered Soft Tissue for Tissue Augmentation

Cited by 21 publications

References 25 publications

Tracking and Increasing Viability of Topically Injected Fibroblasts Suspended in Hyaluronic Acid Filler

Tracking and Increasing Viability of Topically Injected Fibroblasts Suspended in Hyaluronic Acid Filler

Polymeric injectable fillers for cosmetology: Current status, future trends, and regulatory perspectives

History and Applications of Hydrogels

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