Injectable thiol-ene hydrogel of galactoglucomannan and cellulose nanocrystals in delivery of therapeutic inorganic ions with embedded bioactive glass nanoparticles

Wang, Qingbo; Xu, Weihe; Koppolu, Rajesh; Bochove, Bas van; Seppälä, Jukka; Hupa, Leena; Willför, Stefan; Xu, Chunlin; Wang, Xiaoju

doi:10.1016/j.carbpol.2021.118780

Cited by 25 publications

(23 citation statements)

References 59 publications

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“…As described in the Background section, the biocompatibility of CNC itself and CNC hydrogel in vivo has been controversial and not been clearly reported. In most cases, CNC was used as an additive to hydrogel composites to improve their mechanical properties or as a gelation inducer [ 24 , 30 , 31 , 33 , 42 – 44 ], and the reports for biomedical applications and biocompatibility investigations of CNC itself are limited [ 45 ]. In this study, hydrogels composed of only CNCs were subcutaneously injected into mice to evaluate their in vivo biocompatibility, which is the most basic characteristic of a biomaterial scaffold.…”

Section: Discussionmentioning

confidence: 99%

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading

Min

Tae

2023

Biomater Res

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Background Cellular infiltration and angiogenesis into implanted biomaterial scaffolds are crucial for successful host tissue integration and tissue regeneration. Cellulose nanocrystal (CNC) is a nano-sized cellulose derivative, which can form an injectable physical gel with salts. Sulfate groups of sulfated CNC (CNC-S) can act as a binding domain to various growth factors and cytokines with a heparin-binding domain for sustained release of them. Vascular endothelial growth factor (VEGF) can promote the proliferation of endothelial cells and angiogenesis. In this study, VEGF-loaded CNC-S hydrogel was evaluated as an injectable scaffold that can induce cellular infiltration and angiogenesis. Methods CNC-S was hydrolyzed to get desulfated CNC (CNC-DS), which was used as a negative control group against CNC-S. Both CNC-S and CNC-DS hydrogels were prepared and compared in terms of biocompatibility and VEGF release. The hydrogels with or without VEGF loading were subcutaneously injected into mice to evaluate the biocompatibility, cellular infiltration, and angiogenesis induction of the hydrogels. Results Both hydrogels possessed similar stability and shear-thinning behavior to be applicable as injectable hydrogels. However, CNC-S hydrogel showed sustained release (until 8 weeks) of VEGF whereas CNC-DS showed a very fast release of VEGF with a large burst. Subcutaneously injected CNC-S hydrogel showed much enhanced cellular infiltration as well as better biocompatibility with milder foreign body response than CNC-DS hydrogel. Furthermore, VEGF-loaded CNC-S hydrogel induced significant angiogenesis inside the hydrogel whereas VEGF-loaded CNC-DS did not. Conclusion CNC-S possesses good properties as a biomaterial including injectability, biocompatibility, and allowing cellular infiltration and sustained release of growth factors. VEGF-loaded CNC-S hydrogel exhibited efficient angiogenesis inside the hydrogel. The sulfate group of CNC-S was a key for good biocompatibility and the biological activities of VEGF-loaded CNC hydrogel.

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Section: Discussionmentioning

confidence: 99%

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading

Min

Tae

2023

Biomater Res

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“…As a result, a new disulfide bond is formed, while a new thiolate becomes available . The thiolation of polymeric building blocks can be achieved by functionalization of polymer chains with thiol groups − as well as the reduction of disulfide bonds of proteins into thiols . Thiol–disulfide exchange reactions lose their dynamic and reversible nature upon aerial oxidation of thiolate groups, which can impede the self-healing of hydrogels.…”

Section: Design Strategies For Self-healing Injectable Hydrogelsmentioning

confidence: 99%

Self-Healing Injectable Hydrogels for Tissue Regeneration

et al. 2022

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Biomaterials with the ability to self-heal and recover their structural integrity offer many advantages for applications in biomedicine. The past decade has witnessed the rapid emergence of a new class of self-healing biomaterials commonly termed injectable, or printable in the context of 3D printing. These self-healing injectable biomaterials, mostly hydrogels and other soft condensed matter based on reversible chemistry, are able to temporarily fluidize under shear stress and subsequently recover their original mechanical properties. Self-healing injectable hydrogels offer distinct advantages compared to traditional biomaterials. Most notably, they can be administered in a locally targeted and minimally invasive manner through a narrow syringe without the need for invasive surgery. Their moldability allows for a patient-specific intervention and shows great prospects for personalized medicine. Injected hydrogels can facilitate tissue regeneration in multiple ways owing to their viscoelastic and diffusive nature, ranging from simple mechanical support, spatiotemporally controlled delivery of cells or therapeutics, to local recruitment and modulation of host cells to promote tissue regeneration. Consequently, self-healing injectable hydrogels have been at the forefront of many cutting-edge tissue regeneration strategies. This study provides a critical review of the current state of self-healing injectable hydrogels for tissue regeneration. As key challenges toward further maturation of this exciting research field, we identify (i) the trade-off between the self-healing and injectability of hydrogels vs their physical stability, (ii) the lack of consensus on rheological characterization and quantitative benchmarks for self-healing injectable hydrogels, particularly regarding the capillary flow in syringes, and (iii) practical limitations regarding translation toward therapeutically effective formulations for regeneration of specific tissues. Hence, here we (i) review chemical and physical design strategies for self-healing injectable hydrogels, (ii) provide a practical guide for their rheological analysis, and (iii) showcase their applicability for regeneration of various tissues and 3D printing of complex tissues and organoids.

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“…In addition, the fraction of renewable material will be significantly higher in this case where both individual networks are based on AcGGM and from these perspectives the thiol-ene crosslinking strategy may be more viable and the resulting IPNs more suitable as membranes, absorbants and supports serving in more or less pressurized environments. Wang et al (2022b) propose an injectable nanocomposite hydrogel that prepared by light-induced thiol-ene addition between methacrylate modified O-acetyl-galactoglucomannan (GGMMA) and thiolated cellulose nanocrystal (CNC-SH). CNC-SH reinforced the GGMMA hydrogel as both a nanofiller and a crosslinker to GGMMA resulting in an interpenetrating network via thiol-ene addition.…”

Section: Click Chemistrymentioning

confidence: 99%

“… Wang et al (2022b) propose an injectable nanocomposite hydrogel that prepared by light-induced thiol-ene addition between methacrylate modified O-acetyl-galactoglucomannan (GGMMA) and thiolated cellulose nanocrystal (CNC-SH). CNC-SH reinforced the GGMMA hydrogel as both a nanofiller and a crosslinker to GGMMA resulting in an interpenetrating network via thiol-ene addition.…”

Section: Preparation Of Hemicellulose-based Hydrogelsmentioning

confidence: 99%

“…After 24 h, the release of 5-FU and curcumin in PBS was 56% and 37%, which showed a favorable potential in the field of controlled drug release. Wang et al (2022b) introduced BaGNP into the GGMMA/CNC-SH-based injectable hydrogel prepared through light-induced thiol-ene reaction. BaGNP is a kind of nano-filler with good biocompatibility.…”

Section: The Applications Of Hemicellulose-based Hydrogelsmentioning

confidence: 99%

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Hemicellulose-based hydrogels for advanced applications

Liu

Yang

et al. 2023

Front. Bioeng. Biotechnol.

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Hemicellulose-based hydrogels are three-dimensional networked hydrophilic polymer with high water retention, good biocompatibility, and mechanical properties, which have attracted much attention in the field of soft materials. Herein, recent advances and developments in hemicellulose-based hydrogels were reviewed. The preparation method, formation mechanism and properties of hemicellulose-based hydrogels were introduced from the aspects of chemical cross-linking and physical cross-linking. The differences of different initiation systems such as light, enzymes, microwave radiation, and glow discharge electrolytic plasma were summarized. The advanced applications and developments of hemicellulose-based hydrogels in the fields of controlled drug release, wound dressings, high-efficiency adsorption, and sensors were summarized. Finally, the challenges faced in the field of hemicellulose-based hydrogels were summarized and prospected.

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Injectable thiol-ene hydrogel of galactoglucomannan and cellulose nanocrystals in delivery of therapeutic inorganic ions with embedded bioactive glass nanoparticles

Cited by 25 publications

References 59 publications

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading

Self-Healing Injectable Hydrogels for Tissue Regeneration

Hemicellulose-based hydrogels for advanced applications

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