Injectable Platelet-Rich Fibrin as a Drug Carrier Increases the Antibacterial Susceptibility of Antibiotic—Clindamycin Phosphate

Egle, Karina; Skadiņš, Ingus; Grava, Andra; Micko, Lana; Dubniks, Viktors; Šalma, Ilze; Dubņika, Arita

doi:10.3390/ijms23137407

Cited by 6 publications

(6 citation statements)

References 48 publications

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“…Through the reasonable combination of these platelet derivatives, a synergistic potential can be obtained, or, in some cases, certain side effects can be reduced. Moreover, the fibrin matrix can serve as a carrier for certain substances [ 15 , 29 ]. Consequently, the combination of these concentrates with different substances could be useful in a clinical approach.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have emphasized the possible use of platelet-rich fibrin as a carrier matrix. Egle et al (2022) [ 29 ] have used this product together with clindamycin phosphate (a prodrug of clindamycin without antibacterial activity), and the results demonstrated antimicrobial efficacy against Staphylococcus aureus and Staphylococcus epidermidis [ 29 ]. Thus, we considered it appropriate to combine a platelet concentrate, namely iPRF, with a multifunctional iron glycoprotein, lactoferrin, to obtain an increased antimicrobial potential.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, based on the low speed of centrifugation, PRF was classified in advanced PRF (A-PRF, centrifugation 14 min at 1300 rpm); advanced PRF plus (advanced PRF+, 8 min of centrifugation at 1300 rpm); injectable PRF (iPRF, 3 min of centrifugation at 700 rpm); injectable PRF obtained from males (iPRF M, 4 min of centrifugation at 700 rpm); injectable PRF plus (iPRF+, 5 min of centrifugation at 700 rpm); and advanced liquid PRF, (A-PRF L, 5 min of centrifugation at 1300 rpm) [ 5 , 24 , 26 , 27 , 28 ]. Although the regenerative potential of platelet concentrates has been widely explored since their introduction into regenerative medicine, especially in oral and maxillofacial surgery, only a few studies have investigated their antimicrobial behavior [ 5 , 29 ]. It is well known that leukocytes are essential elements of the immune system, containing a variety of antimicrobial peptides and enzymes [ 11 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Bioactive Potential of Functionalized Injectable Platelet-Rich Plasma

et al. 2023

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Injectable platelet-rich fibrin (iPRF) is a frequently used platelet concentrate used for various medical purposes both in veterinary and human medicine due to the regenerative potential of hard and soft tissues, and also because of its antimicrobial effectiveness. This in vitro study was carried out to assess the cumulative antimicrobial and antibiofilm effect of iPRF functionalized with a multifunctional glycoprotein, human lactoferrin (Lf). Thus, the ability to potentiate cell proliferation was tested on keratinocytes and evaluated by the CCK8 test. The combinations of iPRF and Lf induced an increase in the proliferation rate after 24 h. The average cell viability of treated cultures (all nine variants) was 102.87% ± 1.00, and the growth tendency was maintained even at 48 h. The highest proliferation rate was observed in cultures treated with 7% iPRF in combination with 50 µg/mL of Lf, with an average viability of 102.40% ± 0.80. The antibacterial and antibiofilm activity of iPRF, of human lactoferrin and their combination were tested by agar-well diffusion (Kirby–Bauer assay), broth microdilution, and crystal violet assay against five reference bacterial strains. iPRF showed antimicrobial and antibiofilm potential, but with variations depending on the tested bacterial strain. The global analysis of the results indicates an increased antimicrobial potential at the highest concentration of Lf mixed with iPRF. The study findings confirmed the hypothesized enhanced bioactive properties of functionalized iPRF against both Gram-positive and Gram-negative biofilm-producing bacteria. These findings could be further applied, but additional studies are needed to evaluate the mechanisms that are involved in these specific bioactive properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced Bioactive Potential of Functionalized Injectable Platelet-Rich Plasma

et al. 2023

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“…Compared with pure CLP solution, CLP combined with PRF showed higher antibacterial activity against S. aureus and S. epidermidis with significantly lower minimum bactericidal concentrations (from 1,000 μg/mL to 62 μg/mL). Such a modified PRF-CLP scaffold could provide local antimicrobial activity within the first few hours, reducing the risk of postoperative infection ( Egle et al, 2022 ). Ercan E et al used T-PRF and collagen loaded with doxorubicin (Doxy), commonly used in periodontitis treatment, and its anti-collagenase significantly inhibits tissue destruction.…”

Section: Application Of Prf In Bone Tissue Engineeringmentioning

confidence: 99%

Platelet-rich fibrin as an autologous biomaterial for bone regeneration: mechanisms, applications, optimization

Jia,

You,

Zhu

et al. 2024

Front. Bioeng. Biotechnol.

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Platelet-rich fibrin, a classical autologous-derived bioactive material, consists of a fibrin scaffold and its internal loading of growth factors, platelets, and leukocytes, with the gradual degradation of the fibrin scaffold and the slow release of physiological doses of growth factors. PRF promotes vascular regeneration, promotes the proliferation and migration of osteoblast-related cells such as mesenchymal cells, osteoblasts, and osteoclasts while having certain immunomodulatory and anti-bacterial effects. PRF has excellent osteogenic potential and has been widely used in the field of bone tissue engineering and dentistry. However, there are still some limitations of PRF, and the improvement of its biological properties is one of the most important issues to be solved. Therefore, it is often combined with bone tissue engineering scaffolds to enhance its mechanical properties and delay its degradation. In this paper, we present a systematic review of the development of platelet-rich derivatives, the structure and biological properties of PRF, osteogenic mechanisms, applications, and optimization to broaden their clinical applications and provide guidance for their clinical translation.

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“…The carrier was observed to induce structural changes in the clindamycin, giving rise to a more active compound that significantly decreased the minimal bactericidal concentrations required to eliminate Staphylococcal strains. The researchers attested to the safety of the engineered carrier for human cells in vitro, thus evidencing the system’s potential to reduce the risk of postoperative infection [ 17 ]. Finally, Lee et al proposed the controlled storage and release of nitric oxide from metal organic nanosized frameworks formed from Cu-BTC for prospective uses in drug delivery systems.…”

mentioning

confidence: 99%