Injectable macromolecule-based calcium phosphate bone substitutes

Moussi, Hilel; Weiss, Pierre; Bideau, Jean Le; Gautier, H; Charbonnier, Baptiste

doi:10.1039/d2ma00410k

Cited by 13 publications

(13 citation statements)

References 149 publications

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“…Image segmentation by thresholding to obtain reliable quantitative information is consequently di cult and time-consuming. Therefore, µCT data were processed by neural networks with the segmentation wizard of Dragon y software (ORS, Canada) (7,28). The work ow can be described as follows:…”

Section: Deep Learning Image Segmentationmentioning

confidence: 99%

“…The latter are also known to be of critical in uence on bone formation; hence, a synthetic bioactive-free strategy based on biomaterial-induced bone formation seems to be a promising strategy. This could be reached through the development of innovative cement formulations with improved printability, combining the properties of inorganic cement precursors to organic macromolecules (7).…”

Section: Introductionmentioning

confidence: 99%

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Multiparametric influences of 3D-printed organo-mineral scaffolds on bone regeneration

Touya,

Reiss,

Rouillon

et al. 2023

Preprint

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Background The development of synthetic bone substitutes that equal or exceed the efficacy of autologous grafts remains challenging due to a wide range of factors, including the nature of the bone defect to treated and its environment and the patient’s medical history. This study investigated the impact of the composition, architecture, and bioactive additives of 3D-printed organo-mineral cements on host tissue remineralization. Methods Printable cement pastes were formulated by combining hyaluronic acid and α-tricalcium phosphate or anhydrous trimagnesium phosphate cement precursors. Cementitious scaffolds were printed with rectilinear, triangular and gyroid patterns. After 7 weeks of implantation with or without bone marrow, multiparametric qualitative and quantitative assessments were performed using µCT, SEM, and histology. Results None of the setup strategies was as efficient as autologous cancellous bone graft to repair calvarial defects. Nonetheless, the presence of the scaffolds improved the skull vault closure (independent of the composition or architecture), particularly when the scaffolds were soaked in total bone marrow before implantation. No significant effect of scaffold macroarchitecture was observed on tissue mineralization. Magnesium phosphate-based scaffolds (MgP) seemed to induce higher bone formation than their calcium-phosphate-based (CaP) counterparts. They also displayed quick biodegradation, and sparse remaining material was found after 7 weeks of implantation (vs minor biodegradation for CaP). Conclusions Although further improvements are required to reach clinical settings, this study demonstrated the potential of organo-mineral cements for bone regeneration and highlighted the peculiar properties of MgP-based cements. Future investigations on organo-mineral-based materials should take into consideration the comparative baseline provided by these multiparametric assessments.

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Section: Deep Learning Image Segmentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiparametric influences of 3D-printed organo-mineral scaffolds on bone regeneration

Touya,

Reiss,

Rouillon

et al. 2023

Preprint

Self Cite

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“…[3,4] In contrast to the traditional implantation of scaffolds, the minimally invasive injection strategy provides several advantages, including controlled material amount, in situ solidification to suit injury sites, and minimally invasive surgical approaches, which yield less pain and faster recovery. [5,6] Over the years, different injectable systems have been explored, such as liquid metals, [7,8] inorganic ceramics, [9,10] synthetic and natural polymers. [11][12][13] However, many metals may not be absorbed and are permanent implants, leading to the potential for chronic infection and wear debris.…”

Section: Introductionmentioning

confidence: 99%

3D Stem Cell Spheroids with 2D Hetero‐Nanostructures for In Vivo Osteogenic and Immunologic Modulated Bone Repair

Liu,

Astudillo Potes,

Dashtdar

et al. 2024

Adv Healthcare Materials

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Three‐dimensional (3D) stem cell spheroids have immense potential for various tissue engineering applications. However, current spheroid fabrication techniques encounter cell viability issues due to limited oxygen access for cells trapped within the core, as well as non‐specific differentiation issues due to the complicated environment following transplantation. In this study, we developed functional 3D spheroids using mesenchymal stem cells (MSCs) with two‐dimensional (2D) hetero‐nanostructures (HNS) composed of single‐stranded DNA (ssDNA) binding carbon nanotubes (sdCNT) and gelatin‐bind black phosphorus nanosheets (gBPNS). An osteogenic molecule, dexamethasone (DEX), was further loaded to fabricate an sdCNTgBP‐DEX hetero‐nanostructure. This approach aimed to establish a multi‐functional cell‐inductive 3D spheroid with improved oxygen transportation through hollow nanotubes, stimulated stem cell growth by phosphate ions supplied from BP oxidation, in‐situ immunoregulation, and osteogenesis induction by DEX molecules after implantation. Initial transplantation of the 3D spheroids in rat calvarial bone defect showed in vivo macrophage shifts to an M2 phenotype, leading to a pro‐healing microenvironment for regeneration. Prolonged implantation demonstrated outstanding in vivo neovascularization, osteointegration, and new bone regeneration. Therefore, these engineered 3D spheroids hold great promise for bone repair as they allow for stem cell delivery and provide immunoregulative and osteogenic signals within an all‐in‐one construct.This article is protected by copyright. All rights reserved

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“…12 Commercial IBSs are most of the time composed of either bioceramics, bioglass particles or calcium phosphatebased cements (CPCs) associated with polymeric macromolecules which enhance their intrinsic properties (e.g., mechanical resistance), their capacity to be handled (e.g., injectability, setting time, and in situ cohesivity), and material/host (e.g., cell adhesion, proliferation and differentiation) interactions. 13 Particularly, the addition of gelatin or hyaluronic acid to CPCs has been extensively studied. [14][15][16] Today's IBS research is particularly focused on not only the development of innovative formulations based on tailored functionalized hydrogels and substituted CPCs to optimize bone regeneration but also the biodegradation of the material, which is one of the main current roadblocks in clinical applications.…”

Section: Introductionmentioning

confidence: 99%

“…, cell adhesion, proliferation and differentiation) interactions. 13 Particularly, the addition of gelatin or hyaluronic acid to CPCs has been extensively studied. 14–16…”

Section: Introductionmentioning

confidence: 99%

Probing the microporosity and 3D spatial distribution of calcium phosphate cement/hydrogel biomaterials using FIB/SEM at cryogenic temperatures

et al. 2023

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Injectable macromolecule-based calcium phosphate bone substitutes

Abstract: Injectable bone substitutes (IBS) represent compelling options for bone regenerative medicine as they can be used to optimally fill a complex bone defect through minimally invasive intervention. Since their discovery,...

Cited by 13 publications

References 149 publications

Multiparametric influences of 3D-printed organo-mineral scaffolds on bone regeneration

Multiparametric influences of 3D-printed organo-mineral scaffolds on bone regeneration

3D Stem Cell Spheroids with 2D Hetero‐Nanostructures for In Vivo Osteogenic and Immunologic Modulated Bone Repair

Probing the microporosity and 3D spatial distribution of calcium phosphate cement/hydrogel biomaterials using FIB/SEM at cryogenic temperatures

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