Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

Kim, Hyun Joo; You, Su Jung; Yang, Dae Hyeok; Eun, Jin; Park, Hae Kwan; Kim, Moon Suk; Chun, Heung Jae

doi:10.1039/d0bm00588f

Cited by 36 publications

(17 citation statements)

References 24 publications

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“…COL1, which is involved in ECM formation and is important for bone matrix formation and calcification, showed to be 3.67-fold higher in the S/B. 35 The OCN, which is a noncollagenous protein secreted by osteoblasts, showed to be 3.48-fold higher in the mSF-loaded group. It is presumed that mSF provides relatively strong stiffness and a topologically stable structure that facilitates osteogenesis.…”

Section: Resultsmentioning

confidence: 89%

“…RUNX2, which regulates osteogenic differentiation and proliferation of cells, showed to be 2.32-fold higher in the mSF-loaded spheroids. COL1, which is involved in ECM formation and is important for bone matrix formation and calcification, showed to be 3.67-fold higher in the S/B . The OCN, which is a noncollagenous protein secreted by osteoblasts, showed to be 3.48-fold higher in the mSF-loaded group.…”

Section: Resultsmentioning

confidence: 94%

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Fabrication and Characterization of Silk Fibroin Microfiber-Incorporated Bone Marrow Stem Cell Spheroids to Promote Cell–Cell Interaction and Osteogenesis

et al. 2020

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In this study, silk fibroin microfiber (mSF) was applied to assist spheroid assemblies of rBMSCs (rabbit bone marrow stem cells) (S/B). Alkaline hydrolysis was induced with different times and conditions to manufacture the various sizes of mSF. The mSF was incorporated in the rBMSC with different amounts to optimize proper content for spheroid assembly. The formation of the S/B was confirmed under optical microscopy and SEM. Proliferation and viability were characterized by CCK-8 and live/dead staining. Osteogenesis was analyzed with ALP (alkaline phosphatase) activity studies and real-time polymerase chain reaction. The S/B was successfully produced and displayed uniformly distributed cells and mSF with the presence of a gap in the structure. Proliferation and viability of the S/B significantly increased when compared to rBMSC spheroids (B), which is potentially due to the enhanced transportation of oxygen and nutrients to the cells located in the core region. Additionally, ALP activity and osteogenic markers were significantly upregulated in the optimized S/B under osteogenic media conditions. Overall, a hybrid–spheroid system with a simple 3D cell culture platform provides a potential approach for engineering therapeutic stem cells.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 94%

Fabrication and Characterization of Silk Fibroin Microfiber-Incorporated Bone Marrow Stem Cell Spheroids to Promote Cell–Cell Interaction and Osteogenesis

et al. 2020

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“…S3 (d), up to 7 days for both nanospheres in comparison to control (P < 0.05). Specifically, the early osteo/odontogenic gene marker (COL1A) was upregulated on day 1, while the late osteo/odontogenic gene markers (DMP-1, DSPP, and OCN) were highly expressed on day 3 and 7 [ [62] , [63] , [64] , [65] ]. Taken together, Cu-BGn had similar differentiation potential compared to BGn and their cellular internalization was partially mediated by ATP-dependent micropinocytosis.…”

Section: Resultsmentioning

confidence: 99%

Nanotherapeutics for regeneration of degenerated tissue infected by bacteria through the multiple delivery of bioactive ions and growth factor with antibacterial/angiogenic and osteogenic/odontogenic capacity

El‐Fiqi

Mandakhbayar

et al. 2021

Bioactive Materials

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Therapeutic options are quite limited in clinics for the successful repair of infected/degenerated tissues. Although the prevalent treatment is the complete removal of the whole infected tissue, this leads to a loss of tissue function and serious complications. Herein the dental pulp infection, as one of the most common dental problems, was selected as a clinically relevant case to regenerate using a multifunctional nanotherapeutic approach. For this, a mesoporous bioactive glass nano-delivery system incorporating silicate, calcium, and copper as well as loading epidermal growth factor (EGF) was designed to provide antibacterial/pro-angiogenic and osteo/odontogenic multiple therapeutic effects. Amine-functionalized Cu-doped bioactive glass nanospheres (Cu-BGn) were prepared to be 50–60 nm in size, mesoporous, positive-charged and bone-bioactive. The Cu-BGn could release bioactive ions (copper, calcium and silicate ions) with therapeutically-effective doses. The Cu-BGn treatment to human umbilical vein endothelial cells (HUVEC) led to significant enhancement of the migration, tubule formation and expression of angiogenic gene (e.g. vascular endothelial growth factor, VEGF). Furthermore, the EGF-loaded Cu-BGn (EGF@Cu-BGn) showed pro-angiogenic effects with antibacterial activity against E. faecalis , a pathogen commonly involved in the pulp infection. Of note, under the co-culture condition of HUVEC with E. faecalis , the secretion of VEGF was up-regulated. In addition, the osteo/odontogenic stimulation of the EGF@Cu-BGn was evidenced with human dental pulp stem cells. The local administration of the EGF@Cu-BGn in a rat molar tooth defect infected with E. faecalis revealed significant in vivo regenerative capacity, highlighting the nanotherapeutic uses of the multifunctional nanoparticles for regenerating infected/damaged hard tissues.

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“…Kim found that an injectable hydrogel based on MPEG (methoxy polyethylene glycol) -PCL-RGD could promote osteogenic differentiation of stem cells. Moreover, they suggested that focal adhesion kinase (FAK) protein kinase B (AKT) and FAK extracellular signal-regulated kinase (ERK) also played roles in osteogenic differentiation in the RGD-integrin-mediated pathway ( Kim et al, 2020 ). Burdick ( Burdick and Anseth, 2002 ) thought that compare with 0 mM, 0.5 mM RGD, 5 mM concentration of PEG-DA-RGD hydrogel had a more significant ability to promote cell mineralization.…”

Section: Application Of Rgd In Bone Tissue Engineeringmentioning

confidence: 99%

Function and Mechanism of RGD in Bone and Cartilage Tissue Engineering

Yang

Zhang

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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Bone and cartilage injury is common, tissue engineered scaffolds are potential means to repair. Because most of the scaffold materials used in bone and cartilage tissue engineering are bio-inert, it is necessary to increase the cellular adhesion ability of during tissue engineering reconstruction. The Arginine - Glycine - Aspartic acid (Arg-Gly-Asp, RGD) peptide family is considered as a specific recognition site for the integrin receptors. Integrin receptors are key regulators of cell-cell and cell-extracellular microenvironment communication. Therefore, the RGD polypeptide families are considered as suitable candidates for treatment of a variety of diseases and for the regeneration of various tissues and organs. Many scaffold material for tissue engineering and has been approved by US Food and Drug Administration (FDA) for human using. The application of RGD peptides in bone and cartilage tissue engineering was reported seldom. Only a few reviews have summarized the applications of RGD peptide with alloy, bone cements, and PCL in bone tissue engineering. Herein, we summarize the application progress of RGD in bone and cartilage tissue engineering, discuss the effects of structure, sequence, concentration, mechanical stimulation, physicochemical stimulation, and time stimulation of RGD peptide on cells differentiation, and introduce the mechanism of RGD peptide through integrin in the field of bone and cartilage tissue engineering.

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Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

Abstract:
The aim of this study was to investigate the osteogenic potential of BMSCs seeded on RGD-conjugated methoxy polyethylene glycol-polycaprolactone (MP–RGD) in vitro and in vivo.

Cited by 36 publications

References 24 publications

Fabrication and Characterization of Silk Fibroin Microfiber-Incorporated Bone Marrow Stem Cell Spheroids to Promote Cell–Cell Interaction and Osteogenesis

Fabrication and Characterization of Silk Fibroin Microfiber-Incorporated Bone Marrow Stem Cell Spheroids to Promote Cell–Cell Interaction and Osteogenesis

Nanotherapeutics for regeneration of degenerated tissue infected by bacteria through the multiple delivery of bioactive ions and growth factor with antibacterial/angiogenic and osteogenic/odontogenic capacity

Function and Mechanism of RGD in Bone and Cartilage Tissue Engineering

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Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

Abstract: The aim of this study was to investigate the osteogenic potential of BMSCs seeded on RGD-conjugated methoxy polyethylene glycol-polycaprolactone (MP–RGD) in vitro and in vivo.

Cited by 36 publications

References 24 publications

Fabrication and Characterization of Silk Fibroin Microfiber-Incorporated Bone Marrow Stem Cell Spheroids to Promote Cell–Cell Interaction and Osteogenesis

Fabrication and Characterization of Silk Fibroin Microfiber-Incorporated Bone Marrow Stem Cell Spheroids to Promote Cell–Cell Interaction and Osteogenesis

Nanotherapeutics for regeneration of degenerated tissue infected by bacteria through the multiple delivery of bioactive ions and growth factor with antibacterial/angiogenic and osteogenic/odontogenic capacity

Function and Mechanism of RGD in Bone and Cartilage Tissue Engineering

Contact Info

Product

Resources

About

Abstract:
The aim of this study was to investigate the osteogenic potential of BMSCs seeded on RGD-conjugated methoxy polyethylene glycol-polycaprolactone (MP–RGD) in vitro and in vivo.