Injectable Hydrogels as Biomaterials

Nair, Lakshmi S.; Laurencin, Cato T.; Tandon, Mayank

doi:10.1002/9780470891315.ch6

Cited by 11 publications

(10 citation statements)

References 107 publications

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“…Targeted delivery of therapeutic molecules to the desired area with injectable hydrogels can be carried out by incorporating these agents into the precursor solution. However, it is possible that the bioactive agents incorporated in an injectable hydrogel being damaged by exposing them to toxic agents or inducing non-physiological conditions [11,12]. For instance, 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone-1-one (Irgacure 2959), a typical photoinitiator used for UV crosslinking of photocrosslinkable hydrogels, showed high cytotoxicity even at low dosages to human aortic smooth muscle cells [13].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone-1-one (Irgacure 2959), a typical photoinitiator used for UV crosslinking of photocrosslinkable hydrogels, showed high cytotoxicity even at low dosages to human aortic smooth muscle cells [13]. Moreover, in situ crosslinking of the hydrogels can either result in the leakage of initial precursor solution to the adjacent tissue or blood stream, or blockage of the catheters due to premature polymerization [11,14].…”

Section: Introductionmentioning

confidence: 99%

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Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

2018

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In this study, we fabricated and characterized a smart shear-thinning hydrogel composed of gelatin and laponite for localized drug delivery. We added chitosan (Chi) and poly N-isopropylacrylamide-co-Acrylic acid (PNIPAM) particles to the shear-thinning gel to render it pH-responsive. The effects of total solid weight and the percentage of laponite in a solid mass on the rheological behavior and mechanical properties were investigated to obtain the optimum formulation. The nanocomposite gel and particles were characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), zeta potential, and dynamic light scattering techniques. Finally, release related experiment including degradability, swelling and Rhodamine B (Rd) release at various pH were performed. The results suggest that incorporation of silicate nanoplatelets in the gelatin led to the formation of the tunable porous composite, with a microstructure that was affected by introducing particles. Besides, the optimum formulation possessed shear-thinning properties with modified rheological and mechanical properties which preserved its mechanical properties while incubated in physiological conditions. The release related experiments showed that the shear-thinning materials offer pH-sensitive behavior so that the highest swelling ratio, degradation rate, and Rd release were obtained at pH 9.18. Therefore, this nanocomposite gel can be potentially used to develop pH-sensitive systems.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

2018

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“…However, the need to chemically modify chitosan to exhibit the sol-gel transition could significantly affect its biodegradability and bioactivity [4]. We have previously developed a thermal and pH responsive in situ gelling chitosan solution without chemically modifying chitosan [5,6]. The injectable system was shown to undergo thermo gelation under mild physiological conditions and support the viability of encapsulated cells [5,6].…”

Section: Introductionmentioning

confidence: 99%

Injectable thermogelling chitosan for the local delivery of bone morphogenetic protein

McLaughlin

Cui

Starnes

et al. 2012

J Mater Sci: Mater Med

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The aim of the present study was to evaluate the in vivo biocompatibility of injectable thermo gelling chitosan-ammonium hydrogen phosphate solution (chitosan-AHP) and its efficacy to deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in a bioactive form. The thermogel showed a typical foreign body response upon subcutaneous implantation surrounded by a fibrous capsule. Even at 4 and 8 weeks post implantation, significant neutrophil infiltration was observed within the gel. Chitosan-AHP gel retained most of the loaded rhBMP-2 after a small initial release. The bioactivity of the released protein was demonstrated in vitro by the increase in alkaline phosphatase activity of mouse pre osteoblast cells (MC3T3-E1). Histological and micro-computed tomography (μCT) evaluation showed evidence of ectopic bone formation upon 4 μg/mL rhBMP-2 loaded chitosan-AHP injection. The study demonstrated a neutrophil mediated local tissue response to chitosan-AHP gel and its ability to encapsulate and maintain the bioactivity of rhBMP-2.

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“…26,27 Moreover, injectable hydrogels are highly advantageous for growth factor and cellular delivery due to their hydrophilic nature, viscoelasticity and stimuli-responsiveness. [28][29][30][31][32] Chitosan is a cationic polysaccharide of b(1-4)-linked D-glucosamine and N-acetyl-Dglucosamine and has been extensively investigated as a biomaterial for various biomedical applications, due to its excellent biocompatibility, biodegradability and bioactivities. [33][34][35] Chitosan based thermogels raise significant interest as an injectable vehicle for drug as well as cell delivery applications.…”

Section: Introductionmentioning

confidence: 99%

“…Injectable scaffolds have recently gained attention due to the growing popularity of minimally invasive arthroscopic and orthopaedic procedures . Moreover, injectable hydrogels are highly advantageous for growth factor and cellular delivery due to their hydrophilic nature, viscoelasticity and stimuli‐responsiveness . Chitosan is a cationic polysaccharide of β(1–4)‐linked D‐glucosamine and N‐acetyl‐D‐glucosamine and has been extensively investigated as a biomaterial for various biomedical applications, due to its excellent biocompatibility, biodegradability and bioactivities .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the donor cell contribution in rhBMP‐2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice

Gohil

Kuo

Adams

et al. 2016

J Biomedical Materials Res

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Our current understanding regarding the contribution of donor cells in growth factor and cell based tissue regeneration strategies is limited. The present study attempts to utilize fluorescent protein reporter mice [Col3.6Topaz (enhanced yellow fluorescent protein, EYFP) as host and Col3.6Cyan (enhanced cyan fluorescent protein, ECFP) as donor] to determine donor cell contribution in bone regeneration using a bilateral calvarial defect model. Thermogelling chitosan hydrogels (Chi-AHP) were used as bone marrow stromal cell (BMSC) delivery vehicle in the presence and absence of recombinant human bone morphogenetic protein-2 (rhBMP-2). Co-delivery of rhBMP-2 and donor BMSCs led to a significant increase in bone formation indicating the potential of using the combination approach for improved regeneration. On a cellular level, presence of rhBMP-2 resulted in an increased host cell derived osteoblast infiltration at 8 weeks. However, the new mineralized tissue presented two distinct morphological features based on its cellular origin. Regenerated tissue associated with ECFP positive donor cells showed a woven bone-like structure with diffuse alizarin complexone (AC) label and minimal tartrate resistant acid phosphatase (TRAP) activity, indicating the presence of immature early osteoblast cells depositing mineralized tissue without progressing to a mature lamellar bone. Host derived bone showed sharp mineralization line (AC), strong alkaline phosphatase (ALP) and TRAP activity, indicating the presence of actively mineralizing and remodeling, mature lamellar bone matrix. The study demonstrated the remarkable potential of transgenic reporters to improve our understanding of donor cell contribution during bone formation.

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Injectable Hydrogels as Biomaterials

Cited by 11 publications

References 107 publications

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

Injectable thermogelling chitosan for the local delivery of bone morphogenetic protein

Evaluation of the donor cell contribution in rhBMP‐2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice

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