Injectable hyaluronic acid/hydroxyapatite composite hydrogels as cell carriers for bone repair

Tan, Yunfei; Ma, Lei; Chen, Xiangyu; Ran, Yaqin; Tong, Qiulan; Tang, Liwen; Li, Xudong

doi:10.1016/j.ijbiomac.2022.07.009

Cited by 29 publications

(9 citation statements)

References 49 publications

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“…XRD spectra (Figure S4) confirmed that both hybrids exhibited low-crystalline hydroxyapatite phase, as evidenced by comparison with the standard spectrum of hydroxyapatite (JCPDS 090432) . Our previous study revealed that OHya accounted for approximately 22% of the composition in OHAH hybrids . The microstructure of OHAHs resembled an urchin-like morphology, which was assembled from nanoneedles (Figure S5).…”

Section: Resultsmentioning

confidence: 97%

“…The OHAHs were uniformly interspersed in the matrices network, and the interlaced collagen fibrils were adhered onto the OHAHs, as shown in Figure C,D. The coalescence was largely attributed to the formation of imine linkages between the hydroxyapatite hybrids and collagen. , Based on the SEM images, we further quantified the size distributions for single fibril in the matrices (Figure and Figure S7). The observed fibrils exhibited an inhomogeneous feature with respect to size, where single fibrils and aggregated fibrillar bundles coexisted within the COL and CH hydrogels.…”

Section: Resultsmentioning

confidence: 99%

“…26 Our previous study revealed that OHya accounted for approximately 22% of the composition in OHAH hybrids. 25 The microstructure of OHAHs resembled an urchin-like morphology, which was assembled from nanoneedles (Figure S5).…”

Section: Physicochemical Properties Of Hahs and Ohahsmentioning

confidence: 99%

“…In our previous study, hyaluronic acid (Hya), a constituent of ECM, was used to mediate the synthesis of hydroxyapatite hybrids, and subsequently, the Hya incorporated into hydroxyapatite hybrids was endowed with aldehyde groups, which were chemically active to bond with polymers containing amino groups. 25 When such hybrids with active groups were introduced into collagen precursors, the reconstitution of the collagen fibrillar network would be affected by adding different amounts of hydroxyapatite hybrids as well as probably by formation of imine bonds between amino groups of collagen and aldehyde groups of oxidized hyaluronic acid. It is expected that the Hya-dependent synergic effect would yield hydroxyapatite-containing biomimetic matrices with an easily controlled viscoelasticity.…”

Section: ■ Introductionmentioning

confidence: 99%

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Tunable Stress Relaxing Biomimetic Matrices: Hyaluronan/Hydroxyapatite Hybridization Mediates Assembly of Collagen Fibrils

Tan,

Ma,

Cao

et al. 2023

Biomacromolecules

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The alluring correlations of cellular behaviors with viscoelastic extracellular matrices have driven increasing endeavors directed toward the understanding of mechanical cues on cell growth and differentiation via preparing biomimetic scaffolds/gels with viscoelastic controllability. Indeed, systematic investigations, especially into calcium phosphate-containing biomimetics, are relatively rare. Here, oxidized hyaluronic acid/hydroxyapatite hybrids (OHAHs) were synthesized by hyaluronan-mediated biomimetic mineralization with confined ion diffusion and subsequent oxidization treatment. The collagen self-assembly was applied to fabricate tunable stress relaxing fibrillar matrices in the presence of OHAHs in which the incorporated hyaluronic acid with aldehyde groups acted to improve the component compatibility as well as to supplement the molecular interactions with the occurrence of a Schiff-base reaction. With the addition of varying OHAH contents, the self-assembly behavior of collagen was altered, and the obtained collagen-hybrid (CH) matrices presented a heterogeneous fibrillar structure interspersed with OHAHs, characterized by large fibrillar bundles coexisting with small fibrils. The OHAHs improved the hydrogel stability of pure collagen, and according to rheological and nanoindentation measurements, CH matrices also exhibited tunable stress relaxation rates, following an OHAH concentration-dependent fashion. The proliferation and spreading of MC3T3-E1 cells cultured onto such CH matrices were further found to increase with the stress relaxing rate of the matrices. The present study showed that the introduction of hydroxyapatite incorporated with active hyaluronic acid during collagen reconstitution was a simple and effective strategy to realize the preparation of tunable stress relaxing biomimetic matrices potentially used for further appraising the regulation of mechanical cues on cell behaviors.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Physicochemical Properties Of Hahs and Ohahsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tunable Stress Relaxing Biomimetic Matrices: Hyaluronan/Hydroxyapatite Hybridization Mediates Assembly of Collagen Fibrils

Tan,

Ma,

Cao

et al. 2023

Biomacromolecules

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“…Whilst two to three combinations selected from cHLA, cAG, nHAp, and BMP have been examined as bone-forming materials [ 29 , 30 , 31 , 32 , 33 ], there are few reports utilizing cHLA, cAG, nHAp, and BMP simultaneously as a quaternary composite [ 34 ] for bone formation studies.…”

Section: Introductionmentioning

confidence: 99%

Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation

Hachinohe

Taira

Hoshi

et al. 2023

IJMS

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New bone-forming substitute materials are highly useful in dental implantology. The purpose of this study was to prepare cross-linked hyaluronic acid (cHLA)/cross-linked alkaline gelatin (cAG)/nano-hydroxyapatite (nHAp)/bone morphogenic protein (BMP) constructs; and evaluate their bone-forming capabilities in rat cranial bone defects. The cHLA and cAG liquids processed with an epoxy cross-linker were blended with a 3:1 volume ratio, followed by freeze-drying. The dry composites were further infiltrated with water containing nHAp only (BMP (−)) or with water containing nHAp and BMP (BMP (+)). Prepared wet constructs (BMP (−) and BMP (+)) were implanted in rat cranial bone defects, while defects only were also made, and animals were fed for 8 weeks, followed by subsequent soft X-ray measurements and histological observations. The X-ray results showed that BMP (+) constructs disappeared, though caused inward extension of peripherical bone from defect edges with an increase in length of approximately 24%, larger than those of BMP (−) constructs and defect only with approximately 17% and 8% increments, respectively (p < 0.05). Histological observations of BMP (+) construct samples clearly indicated active bone extension consisting of an array of island-like bones. It was concluded that cHLA/cAG/nHAp/BMP could be used as novel bone-substitute materials.

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Development of hybrid hydrogel to facilitate knee joint movement with an engineering approach

Göncü

2022

J of Applied Polymer Sci

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Hyaline cartilage can be defined as a “biological bearing material” with an engineering approach. Movement causes friction between joints while lubricants provide separation between the two surfaces, reducing or preventing frictional wear. This study describes an injectable hybrid hydrogel consisting of a hyaluronic acid matrix filled with hexagonal boron nitride (hBN) particles that can mimic the rheological properties of joint fluid. hBN is a good lubricant and a strong candidate for biomedical applications with its unique tribological properties. The hypothesis established is that the lubricating property of hBN may alleviate the symptoms of osteoarthritis (OA) and improve the person's life quality. A new formulation and strategy for articular injections are proposed. The focus of the present work is the presentation of the rheological profile of hyaluronic acid‐boron nitride (HA‐hBN) hybrid hydrogels. Structural and physical characterizations have shown that BN‐HA hybrid hydrogels can be produced in injectable form, their compatibility with HA strongly depends on surface modification of BN, and they are promising candidates for the treatment of OA by evaluating the frequency range of walking and running.

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Injectable hyaluronic acid/hydroxyapatite composite hydrogels as cell carriers for bone repair

Cited by 29 publications

References 49 publications

Tunable Stress Relaxing Biomimetic Matrices: Hyaluronan/Hydroxyapatite Hybridization Mediates Assembly of Collagen Fibrils

Tunable Stress Relaxing Biomimetic Matrices: Hyaluronan/Hydroxyapatite Hybridization Mediates Assembly of Collagen Fibrils

Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation

Development of hybrid hydrogel to facilitate knee joint movement with an engineering approach

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