Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering

Ren, Kaixuan; He, Chaoliang; Xiao, Chunsheng; Li, Gao; Chen, Xuesi

doi:10.1016/j.biomaterials.2015.02.026

Cited by 240 publications

(180 citation statements)

References 53 publications

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“…Bioactive factors and cells can easily be encapsulated within hydrogels for tissue engineering applications [2,5,6,38,[55][56][57][58]. The degradation of hydrogel biomaterial platforms increases their network pore size, which regulates the release of loaded bioactive molecules, creates free space for newly forming tissues and enhances transport of oxygen and nutrients to encapsulated cells as well as removal of metabolic waste.…”

Section: Hydrogel Formation and Photodegradationmentioning

confidence: 99%

“…Polymeric hydrogel biomaterials are three-dimentional chemically or physically crosslinked hydrophilic polymer networks that can absorb and retain large quantities of water [2,38,[55][56][57][58]. Hydrogels hold great potential in biomedical and pharmaceutical applications because bioactive factors can be encapsulated within them and subsequently delivered in a localized and controlled manner at desired anatomic locations [38,[55][56][57].…”

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confidence: 99%

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Light-Triggered RNA Release and Induction of Hmsc Osteogenesis Via Photodegradable, Dual-Crosslinked Hydrogels

Huynh

Nguyen

Naris

et al. 2016

Nanomedicine (Lond.)

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Aim:To engineer a photodegradable hydrogel system for actively controlled release of bioactive unmodified RNA at designated time points to induce hMSC osteogenesis. Materials & methods: RNA/polyethylenimine complexes were loaded into dual-crosslinked photodegradable hydrogels to examine the capacity of UV light application to trigger their release. The ability of released RNA to drive hMSC osteogenic differentiation was also investigated. Results & conclusion: RNA release from photodegradable hydrogels was accelerated upon UV application, which was not observed in non-photodegradable hydrogels. Regardless of the presence of UV light, released siGFP exhibited high bioactivity by silencing GFP expression in HeLa cells. Importantly, siNoggin or miRNA-20a released from the hydrogels induced hMSC osteogenesis. This system provides a potentially valuable physician/patient-controlled 'on-demand' RNA delivery platform for biomedical applications.

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Section: Hydrogel Formation and Photodegradationmentioning

confidence: 99%

mentioning

confidence: 99%

Light-Triggered RNA Release and Induction of Hmsc Osteogenesis Via Photodegradable, Dual-Crosslinked Hydrogels

Huynh

Nguyen

Naris

et al. 2016

Nanomedicine (Lond.)

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“…The most widely used are the simple chain polymer, such as, polyethylene glycol (PEG), polyacrylic acid (PAA), polyvinyl alcohol (PVA), which have clear structure and modification [22,38] . Another important kind is the derivative polymer from the nature, polypeptide and polysaccharide, such as collagen, hyaluronic acid, alginate, gelatin, glucose, chitosan, chitin and cellulose [22,[39][40][41][42][43] . Poly(L-glutamic acid) (PLGA) is one kind of ideal polypeptide, which exhibits nontoxicity, hydrophilicity, biodegradability, and avoiding antigenicity or immunogenicity [44] .…”

Section: Materials Design Criteriamentioning

confidence: 99%

Self-Healing Hydrogels as Biomedical Scaffolds for Cell, Gene and Drug Delivery

Yuan¹,

Wang²

2017

Res. Rev. J Mat. Sci

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“…Injectable hydrogels are particularly convenient materials for in vivo applications. An emerging class of bioinspired polymers for cartilage and bone tissue engineering are gly- copolypeptides that mimic naturally occurring glycoproteins, that have been processed into injectable hydrogels, by enzymatic cross-linking of glycopeptides in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ) [63]. However, hydrogels, due to their isotropic nature and poor mechanical characteristics, cannot fully mimic the zonal hierarchical structure of the native articular cartilage.…”

Section: Scaffolds As Biomimetic Systems Componentmentioning

confidence: 99%

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

Gadjanski

2018

Osteochondral Tissue Engineering

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In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue engineering for potential clinical use, other applications of biomimetic OC TE including formation of the OC tissues to be used as high-fidelity disease/ healing models and as in vitro models for drug toxicity/efficacy evaluation are covered. Highlights Biomimetic OC TE uses "smart" scaffolds able to locally regulate cell phenotypes and dual-flow bioreactors for two sets of conditions for cartilage/ bone Protocols for hierarchical OC grafts engineering should entail mesenchymal condensation for cartilage and vascular component for bone Immunomodulation, low oxygen tension, purinergic signaling, time dependence of stimuli application are important aspects to consider in biomimetic OC TE Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering Ivana GadjanskiAbstract In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue en...

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Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering

Cited by 240 publications

References 53 publications

Light-Triggered RNA Release and Induction of Hmsc Osteogenesis Via Photodegradable, Dual-Crosslinked Hydrogels

Light-Triggered RNA Release and Induction of Hmsc Osteogenesis Via Photodegradable, Dual-Crosslinked Hydrogels

Self-Healing Hydrogels as Biomedical Scaffolds for Cell, Gene and Drug Delivery

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

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