Injectable Dopamine-Modified Poly(ethylene glycol) Nanocomposite Hydrogel with Enhanced Adhesive Property and Bioactivity

Abstract

A synthetic mimic of mussel adhesive protein, dopamine-modified four-armed poly(ethylene glycol) (PEG-D4), was combined with a synthetic nanosilicate, Laponite (Na0.7+(Mg5.5Li0.3Si8)O20(OH)4)0.7–), to form an injectable naoncomposite tissue adhesive hydrogel. Incorporation of up to 2 wt % Laponite significantly reduced the cure time while enhancing the bulk mechanical and adhesive properties of the adhesive due to strong interfacial binding between dopamine and Laponite. The addition of Laponite did not alter the degradation rate and cytocompatibility of PEG-D4 adhesive. On the basis of subcutaneous implantation in rat, PEG-D4 nanocomposite hydrogels elicited minimal inflammatory response and exhibited an enhanced level of cellular infiltration as compared to Laponite-free samples. The addition of Laponite is potentially a simple and effective method for promoting bioactivity in a bioinert, synthetic PEG-based adhesive while simultaneously enhancing its mechanical and adhesive properties.

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

“…The excessive swelling may induce compression on surrounding tissues, which will bring about pain and other harmful effects. 4 We evaluated the fluid uptake capacity of eGels by incubating them in PBS at 37°C. All eGels can absorb and hold water, and the equilibrium is reached within 24 hours ( Figure 6A).…”

“…[1][2][3][4][5][6][7] Firstly, hydrogels can mimic the nature of tissue from the physical, chemical, electrical, and biological properties, and thus provide an appropriate microenvironment for cell growth and drug release. Secondly, the injectability endows hydrogels with many virtues such as improved patient compliance, facile administration via a minimally invasive mode, wide suitability for complex shapes of target sites, and high loading capacity for cells and therapeutic molecules.…”

Synthesis and Properties of Hemostatic and Bacteria-Responsive in Situ Hydrogels for Emergency Treatment in Critical Situations

“…25,26 Recently, Sakai et al designed a 4-arm-PEG hydrogel by combining two symmetrical 4-arm-PEGs. 27 The 4-arm-PEG hydrogel has high mechanical strength with a maximum breaking strength of 9.6 MPa because of its homogeneous structure, which is tough enough to be used as the hemostasis material in critical situations.…”

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

“…Osteoblasts cell proliferation and differentiation also increased with the addition of Laponite. Injectable hydrogel naoncomposite was investigated by combining Laponite and dopamine- modified four-armed poly(ethylene glycol) (PEG-D4) Liu et al [103] The introduction of Laponite did not change the degradability and cytocompatibility of PEG-D4. However, the curing time, mechanical and adhesive properties were significantly increased.…”