Injectable dicalcium phosphate bone cement prepared from biphasic calcium phosphate extracted from lamb bone

Tariq, Usman; Hussain, Rafaqat; Tufail, Kashif; Haider, Zuhaib; Tariq, Rizwan; Ali, Jalil

doi:10.1016/j.msec.2019.109863

Cited by 18 publications

(10 citation statements)

References 40 publications

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“…Then, after soaking the mMBG/CPC composite of 10 wt.% mMBG/CPC in Tris-buffer for 1 day, the O-P-O (ν 4 mode in PO 4 3– ) bending vibrations of HA can be seen at 563 and 603 cm −1 . The asymmetric bending vibration of HA was found at 1041 cm −1 (ν 3 mode in PO 4 3− ) [ 32 , 33 , 34 ]. These bands indicated that the addition of mMBG does not affect the changes in CPC functional group vibrations.…”

Section: Resultsmentioning

confidence: 99%

Injectability, Processability, Drug Loading, and Antibacterial Activity of Gentamicin-Impregnated Mesoporous Bioactive Glass Composite Calcium Phosphate Bone Cement In Vitro

Chu

Huang

et al. 2022

Biomimetics

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Calcium phosphate cement (CPC) is similar to bone in composition and has plasticity, while mesoporous bioactive glass (MBG) has the advantage of releasing Si, which can promote osteogenic properties and drug loading capacity. A sol–gel-prepared MBG micro-powder (mMBG) and further impregnated antibiotic gentamicin sulfate (Genta@mMBG: 2, 3, and 4 mg/mL) antibiotic were added to CPC at different weight ratios (5, 10, and 15 wt.%) to study CPC’s potential clinical applications. Different ratios of mMBG/CPC composite bone cement showed good injectability and disintegration resistance, but with increasing mMBG addition, the working/setting time and compressive strength decreased. The maximum additive amount was 10 wt.% mMBG due to the working time of ~ 5 min, the setting time of ~ 10 min, and the compressive strength of ~51 MPa, indicating that it was more suitable for clinical surgical applications than the other groups. The 2Genta@mMBG group loaded with 2 mg/mL gentamicin had good antibacterial activity, and the 10 wt.% 2Genta@mMBG/CPC composite bone cement still had good antibacterial activity but reduced the initial release of Genta. 2Genta@mMBG was found to have slight cytotoxicity, so 2Genta@mMBG was composited into CPC to improve the biocompatibility and to endow CPC with more advantages for clinical application.

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Section: Resultsmentioning

confidence: 99%

Injectability, Processability, Drug Loading, and Antibacterial Activity of Gentamicin-Impregnated Mesoporous Bioactive Glass Composite Calcium Phosphate Bone Cement In Vitro

Chu

Huang

et al. 2022

Biomimetics

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“…With a further increase in the proportion of microcapsules, the composites showed a propensity to collapse and decreased compressive strength. Good injectability ( Tariq et al, 2019 ) and self-setting properties facilitate access of CPC to the bone defect area in a minimally invasive manner, allowing it to solidify in situ to adapt to the shape of the cavity. Due to its anti-washout ability, CPC is resistant to loss of bone graft material caused by scouring of body fluid and blood before solidification.…”

Section: Discussionmentioning

confidence: 99%

Physicochemical properties of bone marrow mesenchymal stem cells encapsulated in microcapsules combined with calcium phosphate cement and their ectopic bone formation

Yuan

Shen

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Calcium phosphate bone cement (CPC) serves as an excellent scaffold material for bone tissue engineering owing to its good biocompatibility, injectability, self-setting property and three-dimensional porous structure. However, its clinical use is limited due to the cytotoxic effect of its setting reaction on cells and difficulties in degradation into bone. In this study, bone marrow mesenchymal stem cells (BMSCs) were encapsulated in alginate chitosan alginate (ACA) microcapsules and compounded with calcium phosphate bone cement. Changes in the compressive strength, porosity, injectability and collapsibility of CPC at different volume ratios of microcapsules were evaluated. At a 40% volume ratio of microcapsules, the composite scaffold displayed high porosity and injectability with good collapsibility and compressive strength. Cell live/dead double staining, Cell Counting Kit-8 (CCK-8) assays and scanning electron microscopy were used to detect the viability, proliferation and adhesion of cells after cell microcapsules were combined with CPC. The results revealed that cells protected by microcapsules proliferated and adhered better than those that were directly combined with CPC paste, and cell microcapsules could effectively form macropores in scaffold material. The composite was subsequently implanted subcutaneously on the backs of nude mice, and ectopic osteogenesis of the scaffold was detected via haematoxylin-eosin (H&E), Masson’s trichrome and Goldner’s trichrome staining. CPC clearly displayed better new bone formation function and degradability after addition of pure microcapsules and cell microcapsules. Furthermore, the cell microcapsule treatment group showed greater osteogenesis than the pure microcapsule group. Collectively, these results indicate that BMSCs encapsulated in ACA microcapsules combined with CPC composite scaffolds have good application prospects as bone tissue engineering materials.

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“…Consequently, these are attractive biomaterials for potential hard-tissue applications (Tanzawa et al, 2011). Poor injectability and excessively rapid curing times are common limitations preventing the use of calcium phosphate cements-based into widespread clinical settings (Tariq et al, 2019). Therefore, and increased attention is placed on cement manipulation and appropriate setting times to expand its chemotherapeutic and regenerative capacities.…”

Section: Discussionmentioning

confidence: 99%

Development of Natural-Based Bone Cement for a Controlled Doxorubicin-Drug Release

Dewhurst

Scalzone

Buckley

et al. 2020

Front. Bioeng. Biotechnol.

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Osteosarcoma (OS) accounts for 60% of all global bone cancer diagnoses. Intravenous administration of Doxorubicin Hydrochloride (DOXO) is the current form of OS treatment, however, systemic delivery has been linked to the onset of DOXO induced cardiomyopathy. Biomaterials including calcium phosphate cements (CPCs) and nanoparticles (NPs) have been tested as localized drug delivery scaffolds for OS cells. However, the tumor microenvironment is critical in cancer progression, with mesenchymal stem cells (MSCs) thought to promote OS metastasis and drug resistance. The extent of MSC assisted survival of OS cells in response to DOXO delivered by CPCs is unknown. In this study, we aimed at investigating the effect of DOXO release from a new formulation of calcium phosphate-based bone cement on the viability of OS cells cocultured with hMSC in vitro. NPs made of PLGA were loaded with DOXO and incorporated in the formulated bone cement to achieve local drug release. The inclusion of PLGA-DOXO NPs into CPCs was also proven to increase the levels of cytotoxicity of U2OS cells in mono-and coculture after 24 and 72 h. Our results demonstrate that a more effective localized DOXO delivery can be achieved via the use of CPCs loaded with PLGA-DOXO NPs compared to CPCs loaded with DOXO, by an observed reduction in metabolic activity of U2OS cells in indirect coculture with hMSCs. The presence of hMSCs offer a degree of DOXO resistance in U2OS cells cultured on PLGA-DOXO NP bone cements. The consideration of the tumor microenvironment via the indirect inclusion of hMSCs in this study can act as a starting point for future direct coculture and in vivo investigations.

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Injectable dicalcium phosphate bone cement prepared from biphasic calcium phosphate extracted from lamb bone

Cited by 18 publications

References 40 publications

Injectability, Processability, Drug Loading, and Antibacterial Activity of Gentamicin-Impregnated Mesoporous Bioactive Glass Composite Calcium Phosphate Bone Cement In Vitro

Injectability, Processability, Drug Loading, and Antibacterial Activity of Gentamicin-Impregnated Mesoporous Bioactive Glass Composite Calcium Phosphate Bone Cement In Vitro

Physicochemical properties of bone marrow mesenchymal stem cells encapsulated in microcapsules combined with calcium phosphate cement and their ectopic bone formation

Development of Natural-Based Bone Cement for a Controlled Doxorubicin-Drug Release

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