Injectable Cucurbit[8]uril-Based Supramolecular Gelatin Hydrogels for Cell Encapsulation

Madl, Amy C.; Madl, Christopher M.; Myung, David

doi:10.1021/acsmacrolett.0c00184

Cited by 34 publications

(24 citation statements)

References 63 publications

(130 reference statements)

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“…It has been reported that adding CB[8] into polysaccharides with pendant phenylalanine moieties would form injectable supramolecular hydrogels with superior biocompatibility. [ 42,43 ] However, the reversible interactions as structural cross‐links in hydrogels are mechanically weak due to the nature of secondary bonds. To address this issue, irreversible covalent interactions were also introduced by modifying HA with methacrylate groups in addition to phenylalanine‐cysteine dipeptides (Figure S1, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

3D Printed Biocatalytic Living Materials with Dual‐Network Reinforced Bioinks

Liu

et al. 2021

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The field of living materials seeks to harness living cells as microfactories that can construct a material itself or enhance the performance of material in some manner. While recent advances in 3D printing allow microbe manipulation to create bespoke living materials, the effective coupling of these living components in reinforced bioink designs remains a major challenge due to the difficulty in building a robust and cell‐friendly microenvironment. Here, a type of dual‐network bioink is reported for the 3D printing of living materials with enhanced biocatalysis capabilities, where bioinks are readily printable and provide a biocompatible environment along with desirable mechanical performance. It is demonstrated that integrating microbes into these bioinks enables the direct printing of catalytically living materials with high cell viability and maintains metabolic activity, which those living materials can be preserved and reused. Further, a bacteria‐algae coculture system is fabricated for the bioremediation of chemicals, giving rise to its potential field applications.

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Section: Resultsmentioning

confidence: 99%

3D Printed Biocatalytic Living Materials with Dual‐Network Reinforced Bioinks

Liu

et al. 2021

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“…76,77 Previous studies have addressed this issue using materials that leverage physical cross-linking since their compliant mechanical properties support nonuniform network deformation. 40,68,78 In these nonuniform matrices, mechanical stresses imposed on the material do not induce uniform strain fields, allowing network deformations to dissipate stress. 8 Therefore, we anticipated that the guest−host fibers could mitigate cell-damaging forces during injection due to the force dissipation capabilities of shear-thinning materials.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Guest–Host Supramolecular Assembly of Injectable Hydrogel Nanofibers for Cell Encapsulation

Miller

Hansrisuk

Highley

et al. 2021

ACS Biomater. Sci. Eng.

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The fibrous architecture of the extracellular matrix (ECM) is recognized as an integral regulator of cell function. However, there is an unmet need to develop mechanically robust biomaterials mimicking nanofibrous tissue topography that are also injectable to enable minimally invasive delivery. In this study, we have developed a fibrous hydrogel composed of supramolecularly assembled hyaluronic acid (HA) nanofibers that exhibits mechanical integrity, shear-thinning behavior, rapid self-healing, and cytocompatibility. HA was modified with methacrylates to permit fiber photo-cross-linking following electrospinning and either “guest” adamantane or “host” β-cyclodextrin groups to guide supramolecular fibrous hydrogel assembly. Analysis of fibrous hydrogel rheological properties showed that the mixed guest–host fibrous hydrogel was more mechanically robust (6.6 ± 2.0 kPa, storage modulus (G′)) than unmixed guest hydrogel fibers (1.0 ± 0.1 kPa) or host hydrogel fibers (1.1 ± 0.1 kPa) separately. The reversible nature of the guest–host supramolecular interactions also allowed for shear-thinning and self-healing behavior as demonstrated by cyclic deformation testing. Human mesenchymal stromal cells (hMSCs) encapsulated in fibrous hydrogels demonstrated satisfactory viability following injection and after 7 days of culture (>85%). Encapsulated hMSCs were more spread and elongated when cultured in viscoelastic guest–host hydrogels compared to nonfibrous elastic controls, with hMSCs also showing significantly decreased circularity in fibrous guest–host hydrogels compared to nonfibrous guest–host hydrogels. Together, these data highlight the potential of this injectable fibrous hydrogel platform for cell and tissue engineering applications requiring minimally invasive delivery.

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“…To overcome CB [8]'s solubility limitations, the Scherman and Zhang groups have independently reported the photo-initiated in situ polymerization of CB [8] supramolecular hydrogel networks from monomer precursor solutions [84][85][86][87][88]. Building on this work, we recently reported the fabrication of CB [8]-based supramolecular gelatin hydrogels for cell encapsulation [89]. These optically transparent, shear thinning, injectable hydrogels form on demand via thiol-ene reactions between preassembled CB [8]•FGGC peptide ternary complexes and grafted norbornenes and promote encapsulated cell viability over at least seven days in culture.…”

Section: Cucurbit[n]uril-based Hydrogelsmentioning

confidence: 99%

Supramolecular Host–Guest Hydrogels for Corneal Regeneration

Madl

Myung

2021

Gels

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Over 6.2 million people worldwide suffer from moderate to severe vision loss due to corneal disease. While transplantation with allogenic donor tissue is sight-restoring for many patients with corneal blindness, this treatment modality is limited by long waiting lists and high rejection rates, particularly in patients with severe tissue damage and ocular surface pathologies. Hydrogel biomaterials represent a promising alternative to donor tissue for scalable, nonimmunogenic corneal reconstruction. However, implanted hydrogel materials require invasive surgeries and do not precisely conform to tissue defects, increasing the risk of patient discomfort, infection, and visual distortions. Moreover, most hydrogel crosslinking chemistries for the in situ formation of hydrogels exhibit off-target effects such as cross-reactivity with biological structures and/or result in extractable solutes that can have an impact on wound-healing and inflammation. To address the need for cytocompatible, minimally invasive, injectable tissue substitutes, host–guest interactions have emerged as an important crosslinking strategy. This review provides an overview of host–guest hydrogels as injectable therapeutics and highlights the potential application of host–guest interactions in the design of corneal stromal tissue substitutes.

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Injectable Cucurbit[8]uril-Based Supramolecular Gelatin Hydrogels for Cell Encapsulation

Cited by 34 publications

References 63 publications

3D Printed Biocatalytic Living Materials with Dual‐Network Reinforced Bioinks

3D Printed Biocatalytic Living Materials with Dual‐Network Reinforced Bioinks

Guest–Host Supramolecular Assembly of Injectable Hydrogel Nanofibers for Cell Encapsulation

Supramolecular Host–Guest Hydrogels for Corneal Regeneration

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