Injectable Col-Ⅰ/CS hydrogel enhances bone regeneration in mice tibial mono-cortical defect with impaired osteogenesis

Hydrogel Tissue Bioengineered Scaffolds in Bone Repair: A Review

“…Wang et al [117] used chitosan in combination with β-GP to prepare a temperature-sensitive hydrogel for the promotion of periodontitis repair and bone regeneration, and their research found that berberine thermosensitive hydrogel may be an effective treatment for periodontitis, which can exert anti-inflammatory and osteogenic effects through the PI3K/AKT signaling pathway. Lu et al [118] prepared a thermosensitive hybridized hydrogel scaffold using collagen I (Col-I) In a word, photocurable hydrogel may become an excellent scaffold for bone repair, but the demand for photoinitiators limits its application in some fields, and the selection of photoinitiators becomes a key factor in determining the polymerization efficiency and the required light wavelength. Therefore, these factors must be considered before preparing photocurable hydrogels.…”

“…Wang et al [117] used chitosan in combination with β-GP to prepare a temperature-sensitive hydrogel for the promotion of periodontitis repair and bone regeneration, and their research found that berberine thermosensitive hydrogel may be an effective treatment for periodontitis, which can exert anti-inflammatory and osteogenic effects through the PI3K/AKT signaling pathway. Lu et al [118] prepared a thermosensitive hybridized hydrogel scaffold using collagen I (Col-I) and chondroitin sulfate (CS) as a matrix crosslinked with genipin, which has the advantages of being injectable, temperature-responsive, rapidly crosslinked, and biocompatible, which are favorable for clinical applications. In addition, they demonstrated in their previous report that the deletion of Stat3 impaired the osteogenesis of mesenchymal progenitor cells in vivo and in vitro [119], so they explored the bone-repairing effect of the hydrogel by knocking out the Stat3 gene in mice.…”