Injectable calcium phosphate scaffold and bone marrow graft for bone reconstruction in irradiated areas: An experimental study in rats

A comparison between bone reconstruction following the use of mesenchymal stem cells and total bone marrow in association with calcium phosphate scaffold in irradiated bone

“…The bone marrow myelographic analysis was in agreement with previously published data in rats [5] showing that the bone marrows used for transplantation were normal.…”

“…The animal model has already been used in previous studies [5] and has been developed to mimic the side-effects of irradiation on bone. Lewis 1A-haplotype RT1 a inbred rats were selected because they develop tolerance to bone marrow allografts.…”

“…However, unlike non-irradiated bone, in which calcium phosphate appears to be clinically relevant to autogenous bone grafting [4], calcium phosphate alone is not sufficient to reconstruct bone in irradiated areas. Previous studies have shown that biphasic calcium phosphate (BCP) associated with total bone marrow (TBM) provides better bone reconstruction than TBM or BCP alone in irradiated bone [5,6].…”

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

“…[92] In these cases not only is there a large segmental loss of bone but the regenerative capacity of the adjacent tissue is also impaired because of vasculature damage by radiation treatment. [92,131] The work of Kaigler at el. [128] addressed this problem by delivering VEGF from PLGA scaffolds in irradiated osseous defects.…”

Reconstruction mandibulaire en cancérologie : état actuel et perspectives

“…De plus, l'irradiation postopé ratoire le plus souvent né cessaire compromettrait le potentiel osté o-inducteur de ces biomaté riaux [72,73]. Pour pallier ces deux é cueils, des modè les expé rimentaux de reconstruction osseuse par cé ramique phosphocalcique puis greffe autologue de moelle osseuse aprè s irradiation ont é té ré cemment dé veloppé s [74][75][76]. Le sché ma associe successivement : reconstruction par biomaté riaux, irradiation, injection percutané e de moelle osseuse autologue.…”