Injectable and reversible preformed cryogels based on chemically crosslinked gelatin methacrylate (GelMA) and physically crosslinked hyaluronic acid (HA) for soft tissue engineering

Shariatzadeh, Farinaz Jonidi; Solouk, Atefeh; Khoulenjani, Shadab Bagheri; Bonakdar, Shahin; Mirzadeh, Hamid

doi:10.1016/j.colsurfb.2021.111725

Cited by 29 publications

(34 citation statements)

References 41 publications

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“…Thirdly, the same RGD structural features represent the target sequences of matrix metalloproteinases (MMPs) which make both collagen and gelatin easily biodegradable [ 20 ]. For the above reasons, CBCs and GBCs have been considered as promising materials in biomedical fields, particularly in cell culture [ 21 , 22 ], tissue engineering [ 23 , 24 , 25 , 26 ], drug delivery [ 27 , 28 , 29 ], skin wound repair [ 30 ], etc.…”

Section: Introductionmentioning

confidence: 99%

An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications

Wang

et al. 2021

Polymers

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Decades of research into cryogels have resulted in the development of many types of cryogels for various applications. Collagen and gelatin possess nontoxicity, intrinsic gel-forming ability and physicochemical properties, and excellent biocompatibility and biodegradability, making them very desirable candidates for the fabrication of cryogels. Collagen-based cryogels (CBCs) and gelatin-based cryogels (GBCs) have been successfully applied as three-dimensional substrates for cell culture and have shown promise for biomedical use. A key point in the development of CBCs and GBCs is the quantitative and precise characterization of their properties and their correlation with preparation process and parameters, enabling these cryogels to be tuned to match engineering requirements. Great efforts have been devoted to fabricating these types of cryogels and exploring their potential biomedical application. However, to the best of our knowledge, no comprehensive overviews focused on CBCs and GBCs have been reported currently. In this review, we attempt to provide insight into the recent advances on such kinds of cryogels, including their fabrication methods and structural properties, as well as potential biomedical applications.

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Section: Introductionmentioning

confidence: 99%

An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications

Wang

et al. 2021

Polymers

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“…At these temperatures, water freezes and its crystals act as a porogen. Thawing of crystals at room temperature after gelation results in the formation of an interconnected macroporous (90% porosity [ 81 ]) structure permitting the flow of nutrients and cell trafficking thus facilitating tissue integration ( Figure 7 ). The latter is especially important for tissues lacking blood vessels such as the articular cartilage.…”

Section: Hydrogels—preclinical Evaluationmentioning

confidence: 99%

“…Cryogels are also characterized by their shape-memory properties and integrity due to their adequate degree of swelling and mechanical strength and elasticity. The use of hyaluronic acid (HA)-based cryogels as cell carriers for the repair of cartilage defects is considered promising due to their biocompatibility/biodegradability and their shape-memory properties (i.e., they contract and recover their shape after syringe injection) allowing their noninvasive topical administration via injection [ 81 , 82 , 83 ].…”

Section: Hydrogels—preclinical Evaluationmentioning

confidence: 99%

“…The above demonstrated the potential of the injectable chondrocyte-laden cryogels to promote cartilage tissue regeneration. In another study, Shariatzadeh and co-workers [ 81 ] applied cryogelation for the fabrication of highly porous interpenetrating networks (IPNs) based on gelatin methacrylate (GelMA) (3 wt%) and HA (5–20 wt%). The developed cryogels were found to be mechanically stable to applied compression (up to 90%) and to exhibit shape-memory properties.…”

Section: Hydrogels—preclinical Evaluationmentioning

confidence: 99%

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Recent Developments in Hyaluronic Acid-Based Hydrogels for Cartilage Tissue Engineering Applications

2022

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Articular cartilage lesions resulting from injurious impact, recurring loading, joint malalignment, etc., are very common and encompass the risk of evolving to serious cartilage diseases such as osteoarthritis. To date, cartilage injuries are typically treated via operative procedures such as autologous chondrocyte implantation (ACI), matrix-associated autologous chondrocyte implantation (MACI) and microfracture, which are characterized by low patient compliance. Accordingly, cartilage tissue engineering (CTE) has received a lot of interest. Cell-laden hydrogels are favorable candidates for cartilage repair since they resemble the native tissue environment and promote the formation of extracellular matrix. Various types of hydrogels have been developed so far for CTE applications based on both natural and synthetic biomaterials. Among these materials, hyaluronic acid (HA), a principal component of the cartilage tissue which can be easily modified and biofunctionalized, has been favored for the development of hydrogels since it interacts with cell surface receptors, supports the growth of chondrocytes and promotes the differentiation of mesenchymal stem cells to chondrocytes. The present work reviews the various types of HA-based hydrogels (e.g., in situ forming hydrogels, cryogels, microgels and three-dimensional (3D)-bioprinted hydrogel constructs) that have been used for cartilage repair, specially focusing on the results of their preclinical and clinical assessment.

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“…Although these cryogels had shown no failure up to 90% compression, they had a complicated fabrication process. 15,16 Using unsaturated double bonds may create some drawbacks, especially when they do not participate in the crosslinking reactions, and these active sites cause the risk of toxicity. The versatility in a preparation method, biocompatibility, and injectability are the challenge which we aimed to respond in comparison to other cryogels based on CMC and Ge.…”

Section: Introductionmentioning

confidence: 99%

Shape memory injectable cryogel based on carboxymethyl chitosan/gelatin for minimally invasive tissue engineering: In vitro and in vivo assays

Olov

Mirzadeh

Moradi³

et al. 2022

J Biomed Mater Res

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Shape‐memory cryogels have drawn attention as an injectable system to minimize the risks associated with surgical implantation in tissue engineering. To achieve shape memory behavior with hydration as an external stimulus, it is necessary to have a porous elastic network. To achieve this, it is crucial to control the crosslinking process at the time of pore formation, especially for natural‐based polymers. In this study, a versatile method using a cryogelation method in the presence of chemical and physical crosslinkers is investigated to obtain an injectable super macroporous elastic structure based on a poly(ampholyte) (carboxymethyl chitosan) and a protein (gelatin). Mechanical, swelling, shape memorizing behavior, injectability, and in vitro and in vivo behavior of cryogels were studied. Cryogelation in a subzero temperature led to the formation of scaffolds with interconnected pores of the size of 350 μm which swelled completely after 3 min. Cryogels had crosslink density up to 22% and elastic modulus in the hydrated state up to 0.054 and 1.733 MPa at low and high strains, respectively, and low hysteresis (<30 kPa). Injectability studies confirmed the ability of the cryogels to be injected through a 16G needle. In vitro studies demonstrated good cellular penetration, cell adhesion, and high cell viability (>100%). In vivo studies using mice showed that the body's response was befitting without inflammation and any side effect for the liver and kidneys.

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Injectable and reversible preformed cryogels based on chemically crosslinked gelatin methacrylate (GelMA) and physically crosslinked hyaluronic acid (HA) for soft tissue engineering

Cited by 29 publications

References 41 publications

An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications

An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications

Recent Developments in Hyaluronic Acid-Based Hydrogels for Cartilage Tissue Engineering Applications

Shape memory injectable cryogel based on carboxymethyl chitosan/gelatin for minimally invasive tissue engineering: In vitro and in vivo assays

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