Injectable and in situ gelling hydrogels for modified protein release

Pescosolido, Laura; Miatto, S.; Meo, Chiara Di; Cencetti, Claudia; Coviello, Tommasina; Alhaique, Franco; Matricardi, Pietro

doi:10.1007/s00249-009-0440-2

Cited by 31 publications

(20 citation statements)

References 24 publications

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“…A 3 D 10 8 and A 3 D 20 8 IPNs showed complete degradation in about 15-18 days, depending on the polymer concentration. The A 3 D 12 10 and A 3 D 12 20 IPNs fully degraded in about 30 and 60 days, respectively, while the IPNs with the highest DS degraded in 70-180 days. As expected, increasing both the dex-HEMA concentration and the DS resulted in increased degradation times, which can be attributed to the higher cross-link density of the IPNs [22,27].…”

Section: Physico-chemical Characterization Of Hydrogelsmentioning

confidence: 96%

“…The physico-chemical characteristics of the semi-IPNs (Alg-Ca and dex-MA before UV curing) and of the corresponding IPNs (i.e. after UV curing) were described [19,20]. These systems showed interesting properties including injectability, improved mechanical strength in comparison to the two separate hydrogel systems, arising from the synergistic interaction between the polymer networks, and tunable protein release properties.…”

Section: Introductionmentioning

confidence: 98%

“…Recently, we described IPN hydrogels based on calcium alginate (Alg-Ca) and dextran methacrylate derivatives (dex-MA) [19,20]. The physico-chemical characteristics of the semi-IPNs (Alg-Ca and dex-MA before UV curing) and of the corresponding IPNs (i.e.…”

Section: Introductionmentioning

confidence: 99%

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In situ forming IPN hydrogels of calcium alginate and dextran-HEMA for biomedical applications

et al. 2011

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In situ forming hydrogels, which allow for the modulation of physico-chemical properties, and in which cell response can be tailored, are providing new opportunities for biomedical applications. Here, we describe interpenetrating polymer networks (IPNs) based on a physical network of calcium alginate (Alg-Ca), interpenetrated with a chemical one based on hydroxyethyl-methacrylate-derivatized dextran (dex-HEMA). IPNs with different concentration and degree of substitution of dex-HEMA were characterized and evaluated for protein release as well as for the behavior of embedded cells. The results demonstrated that the properties of the semi-IPNs, which are obtained by dissolution of dex-HEMA chains into the Alg-Ca hydrogels, would allow for injection of these hydrogels. Degradation times of the IPNs after photocross-linking could be tailored from 15 to 180 days by the concentration and the degree of substitution of dex-HEMA. Further, after an initial burst release, bovine serum albumin was gradually released from the IPNs over approximately 15 days. Encapsulation of expanded chondrocytes in the IPNs revealed that cells remained viable and, depending on the composition, were able to redifferentiate, as was demonstrated by the deposition of collagen type II. These results demonstrate that these IPNs are attractive materials for pharmaceutical and biomedical applications due to their tailorable mechanical and degradation characteristics, their release kinetics and biocompatibility.

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Section: Physico-chemical Characterization Of Hydrogelsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 98%

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In situ forming IPN hydrogels of calcium alginate and dextran-HEMA for biomedical applications

et al. 2011

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“…2. For not cross-linked beads a decrease in F max is observed as Dex-MA concentration increase, because of the possible interference of Dex-MA chains on Ca-Alg ''egg boxes'', [45,46] that leads to a reduction of the polymer network strength. On the other side, for the crosslinked beads, both the F max and the E cohes increase as Dex-MA concentration in the beads increases.…”

Section: Mechanical Propertiesmentioning

confidence: 94%

“…For the samples not UV crosslinked, this density, on the contrary, decreases, supporting the hypothesis of the disturbing effect of methacrylate moieties on the ''egg box'' formation between Ca 2? and alginate chains [45,46]. …”

Section: Rheological Analysismentioning

confidence: 99%

Calcium alginate/dextran methacrylate IPN beads as protecting carriers for protein delivery

D’Arrigo

Meo

Pescosolido

et al. 2012

J Mater Sci: Mater Med

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In the present study, mechanical and protein delivery properties of a system based on the interpenetration of calcium-alginate (Ca-Alg) and dextran-methacrylate (Dex-MA) networks are shown. Interpenetrated hydrogels beads were prepared by means of the alginate chains crosslinking with calcium ions, followed by the exposure to UV light that allows the Dex-MA network formation. Optical microscope analysis showed an average diameter of the IPN beads (Ca-Alg/Dex-MA) of 2 mm. This dimension was smaller than that of Ca-Alg beads because of the Dex-MA presence. Moreover, the strength of the IPN beads, and of their corresponding hydrogels, was influenced by the Dex-MA concentration and the crosslinking time. Model proteins (BSA and HRP) were successfully entrapped into the beads and released at a controlled rate, modulated by changing the Dex-MA concentration. The enzymatic activity of HRP released from the beads was maintained. These novel IPN beads have great potential as protein delivery system.

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