Initiation of skin basement membrane formation involves assembly of laminin-1 and laminin-5 through binding to cell membrane receptors

Fleischmaier, Raul; MacDonald, Ewan; Perlish, Jerome S.; Sapadin, Allen N.; Yomada, Yoshihiko

doi:10.1016/s0923-1811(98)83716-x

Cited by 43 publications

(59 citation statements)

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“…The BM is known to be a critical signal for the control of normal epidermal growth (Fleischmajer et al 1998;Marinkovich et al 1993) and establishes basal cell polarity associated with hemidesmosomes (Dowling et al 1996). Our findings support these observations, as only tissues grown on substrates such as Type IV Collagen, that was permissive for improved BM organization and improved epithelial tissue architecture.…”

Section: Discussionsupporting

confidence: 85%

“…HSEs constructed by seeding keratinocytes on an interface containing pre-existing BM components, such as de-epithelialized skin (Andriani et al 2003) or amniotic membrane (Yang et al 2006) promote rapid assembly of well-structured BM. This is thought to occur due to interactions between Type IV Collagen and β1 integrins (Fleischmajer et al 1998) that has been shown to provide an early scaffold for BM organization. Our finding that the presence of Type IV Collagen is linked to the assembly of BM supports these previous findings and extends the significance of these events by linking them to the concurrent normalization of epithelial growth, morphogenesis and survival during the early stages of tissue organization.…”

Section: Discussionmentioning

confidence: 99%

“…To accomplish this, tissues were analyzed for the presence of laminin 5, Type VII Collagen, Type IV Collagen and α6 integrin, as markers of BM organization. As laminin 5 and α6 integrin are produced strictly by keratinocytes (Marinkovitch et al, 1993) and are known to be linked to the earliest stages of BM development (Fleischmajer et al, 1998), their distribution would serve as an indicator of how various epithelial-stromal interfaces could enable initiation of BM organization. When keratinocytes were seeded onto the AlloDerm substrate, deposition of laminin 5 ( Figure 5G) and α6 integrin ( Figure 5H), as well as Type IV Collagen and Type VII Collagen ( Figure 6) were found to be linear and strictly polarized along the BM interface.…”

Section: Maturation Of the Bm Zone Is Linked To The Presence Of Type mentioning

confidence: 99%

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The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

Segal¹,

Andriani²,

Pfeiffer³

et al. 2008

Matrix Biology

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Epithelial-mesenchymal interactions promote the morphogenesis and homeostasis of human skin. However, the role of the basement membrane (BM) during this process is not well-understood. To directly study how BM proteins influence epidermal differentiation, survival and growth, we developed novel 3D human skin equivalents (HSEs). These tissues were generated by growing keratinocytes at an air-liquid interface on polycarbonate membranes coated with individual matrix proteins (Type I Collagen, Type IV Collagen or fibronectin) that were placed on contracted Type I Collagen gels populated with dermal fibroblasts. We found that only keratinocytes grown on membranes coated with the BM protein Type IV Collagen showed optimal tissue architecture that was similar to control tissues grown on de-epidermalized dermis (AlloDerm) that contained intact BM. In contrast, tissues grown on proteins not found in BM, such as fibronectin and Type I Collagen, demonstrated aberrant tissue architecture that was linked to a significant elevation in apoptosis and lower levels of proliferation of basal keratinocytes. While all tissues demonstrated a normalized, linear pattern of deposition of laminin 5, tissues grown on Type IV Collagen showed elevated expression of α6 integrin, Type IV Collagen and Type VII Collagen, suggesting induction of BM organization. Keratinocyte differentiation (Keratin 1 and filaggrin) was not dependent on the presence of BM proteins. Thus, Type IV Collagen acts as a critical microenvironmental factor in the BM that is needed to sustain keratinocyte growth and survival and to optimize epithelial architecture.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Maturation Of the Bm Zone Is Linked To The Presence Of Type mentioning

confidence: 99%

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The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

Segal¹,

Andriani²,

Pfeiffer³

et al. 2008

Matrix Biology

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“…Compressed dermis by mechanical stress also could show partial high collagen-density area in the LD. Type IV collagen, type XII collagen and laminin 5 are important structural protein components of the BMZ [2,4,11]. Extracellular accumulation of the type IV collagen results in membrane formation, through self-assembly of a mesh-like sheet, which is ultramicroscopically seen as the lamina densa in the BMZ [1].…”

Section: Discussionmentioning

confidence: 99%

Unilateral Basement Membrane Zone Alteration of the Regenerated Laminar Region in Equine Chronic Laminitis

Kuwano

Ueno

Katayama

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Between the laminar epidermis and the laminar dermis of laminar region (LR) in equine foot, it can be observed the basement membrane zone (BMZ), which is composed of a basement membrane and its accompaniments like the hemidesmosome and anchoring fibril. Alteration in the BMZ in equine laminitis is possibly related with not only development but also recovery outcome and recurrence of this disease. However, there is little known about the structure of the BMZ during the recovery phase of this disease. To assess the condition of the BMZ of LR affected by chronic laminitis, the tissue was examined in three cases at two weeks, four weeks and three months after the onset of laminitis, using pathological, immunohistochemical and electron microscopic techniques. Histologically in all laminitis cases, there was a regenerated laminar epidermis with proliferating keratinocytes between the Stratum medium and the dermis, but it included the undeveloped secondary epidermal laminae (ud-SELs) structure in one side of the primary epidermal laminae, especially in the part of the deep area of LR. Immunohistochemical results were positive for the anti-type IV collagen, anti-type VII collagen and anti-laminin 5 antibodies in the most BMZs. However, partial BMZs adjacent to the ud-SELs were negative for the anti-type VII collagen and anti-laminin 5 antibodies. Ultrastructurally, in the BMZ of the ud-SEL, the lamina densa and the lamina lucida were present. In contrast, the anchoring fibrils and the hemidesmosomes were either absent, or present at lower than normal levels. In conclusion, the present study indicated that the part of regenerated LR in chronic laminitis was not able to fully restore to construct the BMZ for a long time, especially in the unilateral side of laminar epidermis. It might be related with recurrence of this disease. KEY WORDS: basement membrane zone, chronic laminitis, collagen, equine, laminin 5.J. Vet. Med. Sci. 67 (7): [685][686][687][688][689][690][691] 2005 The integumental tissues of the foot are important in supporting the horse's entire body weight during static and dynamic loading conditions. If severe intractable hoof disease occurs, the horse may lose not only the ability to run, but also to stand. Laminitis is one such intractable hoof condition and has a recurrent nature. Many horses have suffered from this disease over extended periods, resulting in significant treatment costs, and therefore the equine industry has a great deal of interest in clarifying the pathogenesis and developing treatment methods [5,9,12].Anatomically, the laminar region in foot displays a complex 3-dimensional structure consisting of the primary epidermal lamina (PEL) and the secondary epidermal lamina (SEL) which interdigitate with their dermal counterparts [24]. These dermal and epidermal components are separated by the basement membrane zone (BMZ), which is composed of a basement membrane and its accompaniments like the hemidesmosome and anchoring fibril [23]. The hemidesmosome, anchoring fibril and other c...

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“…It showed anchoring fibrils and hemidesmosomes at the electron microscopic level, and both collagen type IV and type VII histochemically. Furthermore, we also found the laminin 5␥2 subunit, which has been shown to be a required nucleation site for basement membrane formation (Fleischmajer et al, 1998). It could be that our engineered tissues showed such good basement membrane formation because we allowed the fibroblasts a 2-week phase to remodel the collagen gel into a more receptive matrix before we added the keratinocytes.…”

Section: Discussionmentioning

confidence: 82%

Recapitulation of oral mucosal tissues in long‐term organotypic culture

et al. 2003

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To test the influence of fibroblasts on epithelial morphology and expression of keratinocyte proteins and barrier lipids, we bioengineered homotypic and heterotypic oral mucosae and skin using cultured adult human cells. Fibroblasts were allowed to modify collagen type I gels for 2 weeks before keratinocytes were added. The organotypic cultures were then grown at the air-liquid interface for 4 weeks. In homotypic combinations, epithelial morphology and protein expression closely mimicked those in vivo. In heterotypic combinations, the morphology resembled that in vivo and keratinocytes expressed their typical markers, except when skin keratinocytes were recombined with alveolar fibroblasts; they expressed K19, K4, and K13, which is similar to oral mucosal epithelia rather than to the epidermis. Morphologically, the stratum corneum layers were typical for the epithelial tissues. Grafting the bioengineered cultures to the backs of Nude mice did not change the results, suggesting that our findings are not merely a culture phenomenon. Lipid profiles of the homotypic combinations mimicked the profiles found in the normal epithelial tissues, except that the engineered alveolar epithelium expressed more ceramide 2 than that in vivo. In the heterotypic combinations, keratinocytes appeared to control the lipid profile, except in the combination of skin keratinocytes with alveolar fibroblasts, wherein the ceramide profile appeared to be partly that of alveolar epithelium and partly that of epidermis. These results suggest that cultured adult fibroblasts and keratinocytes are sufficient to recapitulate graftable oral tissues, and, except for alveolar fibroblasts, the type of fibroblast had little influence on keratinocyte differentiation. Anat Rec Part A 270A: 162-174, 2003.

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Initiation of skin basement membrane formation involves assembly of laminin-1 and laminin-5 through binding to cell membrane receptors

Cited by 43 publications

References 0 publications

The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

The basement membrane microenvironment directs the normalization and survival of bioengineered human skin equivalents

Unilateral Basement Membrane Zone Alteration of the Regenerated Laminar Region in Equine Chronic Laminitis

Recapitulation of oral mucosal tissues in long‐term organotypic culture

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