Initiation of Post-Primary Tuberculosis of the Lungs: Exploring the Secret Role of Bone Marrow Derived Stem Cells

Guo

Adv Materials Technologies

et al. 2021

This article describes a technology to transform a petri dish into a microfluidic chip with a stem cell nest for stem cell niche engineering. A permanent magnet sphere is put in a 30 mm petri dish as an oscillator pump (O‐pump). An earphone‐sized actuator outside the petri dish receives the programmed audio signal from an MP3 player or a mobile phone to drive the in‐dish O‐pump for a microflow. There are guiding walls to form a wake area for the cell nest at the dish's center. The cell nest uses the wake to create a stable, ultraslow internal microcirculation flow. The retention half‐life of nanoparticles in the cell nest is 1419.8 ± 1.3 s. By setting the microfluidic program and supplementing five drops of the culture medium outside the cell nest every 2 d, the growth rate of embryonic stem cells in the cell nest during the 8 d automatic culture appears significantly optimized. The single clone area of embryonic stem cells increases from (3.57 ± 0.52) × 105 to (11.67 ± 1.33) × 105 µm2 after 2 d of the automatic culture and advances to (53.34 ± 8.37) × 105 µm2 after 4 d.

Section: Introductionmentioning

confidence: 99%

Design Artificial Stem Cell Nests for Stem Cell Niche in a Microfluidic Petri Dish Programmed by a Cell Phone

Guo

Adv Materials Technologies

et al. 2021

Front. Cell. Infect. Microbiol.

“…Studies have demonstrated that MTB can change the immune function of host cells by secreting different effector proteins at different stages of infection. These results suggest that the immune escape of MTB is closely related to the host cell membrane surface pattern receptor (PPR) ( Pathak and Das, 2020 ). For example, after entering the host, the cell wall components of MTB, such as phosphatidylinositol mannose (PIM), lipoarabinomannan (LAM), and some other outer membrane molecules, are recognized by macrophages through membrane surface pattern receptors.…”

Section: Molecular Mechanism Of Mtb Escape From Host Ppr Signal Trans...mentioning

confidence: 99%

Emerging advances in identifying signal transmission molecules involved in the interaction between Mycobacterium tuberculosis and the host

Wang

Shi

Chen

et al. 2022

Tuberculosis caused by Mycobacterium tuberculosis (MTB) is an ancient chronic infectious disease and is still the leading cause of death worldwide due to a single infectious disease. MTB can achieve immune escape by interacting with host cells through its special cell structure and secreting a variety of effector proteins. Innate immunity-related pattern recognition receptors (PPR receptors) play a key role in the regulation of signaling pathways.In this review, we focus on the latest research progress on related signal transduction molecules in the interaction between MTB and the host. In addition, we provide new research ideas for the development of new anti-tuberculosis drug targets and lead compounds and provide an overview of information useful for approaching future tuberculosis host-oriented treatment research approaches and strategies, which has crucial scientific guiding significance and research value.

“…Therefore, dormant Mtb in cells may promote the reprogramming of MSCs in the lungs of acute respiratory infection (ARI) to the ASCs phenotype. The reprogrammed ASCs can then stimulate the replication of dormant Mtb and subsequently release them to neighboring MSCs and/or macrophages (28). This process will lead to primary TB in the lungs.…”

Section: Mscs and Tg Mscs Regulates Mtb Growthmentioning

confidence: 99%

Mesenchymal Stem Cells and Tuberculosis: Clinical Challenges and Opportunities

Zhang

Xie

et al. 2021

Front. Immunol.

Tuberculosis (TB) is one of the communicable diseases caused by Mycobacterium tuberculosis (Mtb) infection, affecting nearly one-third of the world’s population. However, because the pathogenesis of TB is still not fully understood and the development of anti-TB drug is slow, TB remains a global public health problem. In recent years, with the gradual discovery and confirmation of the immunomodulatory properties of mesenchymal stem cells (MSCs), more and more studies, including our team’s research, have shown that MSCs seem to be closely related to the growth status of Mtb and the occurrence and development of TB, which is expected to bring new hope for the clinical treatment of TB. This article reviews the relationship between MSCs and the occurrence and development of TB and the potential application of MSCs in the treatment of TB.