Initiation and culture of potato tuber callus tissue with picloram

Hagen, Steven R.; LeTourneau, Duane; Muneta, Paul; Brown, J.

doi:10.1007/bf00024919

Cited by 10 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present finding that both leaf and internodal explants of potato (cv. Iwa) exhibited a high frequency of callus formation in response to picloram alone was similar to that in a previous study on Lemhi Russet potato (Hagen et al 1990). In addition, the finding that both leaf and intermodal explants could also form callus in response to BA in combination with NAA was similar to that of a previous study on leaf sections of potato cv.…”

Section: Discussionsupporting

confidence: 83%

“…Many studies have shown that callus induction in potato could be influenced by a variety of factors in- cluding genotype, explant type, and plant growth regulator (type and concentration) (Hagen et al 1990;JayaSree et al 2001;Yee et al 2001;Tican et al 2008;Dokhaniyeh et al 2011;Pal et al 2012). The present finding that both leaf and internodal explants of potato (cv.…”

Section: Discussionmentioning

confidence: 74%

“…It has not been investigated if these morphologically distinct calli would differ in their resistance to trace metal exposure. Here, the possible relationship between callus morphology and trace metal resistance was studied using potato callus cultures initiated on medium supplemented with picloram (Hagen et al 1990), or with a combination of 1-napthalene acetic acid (NAA) and 6-benzyladenine (BA) (JayaSree et al 2001). Their distinct callus morphology was first confirmed and then their responses to exposure to media containing different Cd concentrations were compared.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

et al. 2015

View full text Add to dashboard Cite

Callus culture has been used to study the cellular basis of sensitivity of plants to toxic trace elements including cadmium. Callus friability may be related to plant response to exposure to trace elements as callus friability is related to the plant cell wall which plays a role in resistance of plant cells to trace element toxicity. The aim of this study was to investigate the relationship between two types of potato callus with different friability and their sensitivity to cadmium. A high frequency (about 80%) of leaf or internodal explants of potato (Solanum tuberosum L., cv, Iwa) formed a friable, pale green callus (type-A callus) on half-strength basal Murashige and Skoog medium supplemented with 12.42 µM picloram. On medium supplemented with 4.43 µM 6-benzyladenine (BA) and 5.37 µM 1-naphthalene acetic acid (NAA), a compact, non-friable callus (type-B callus) was induced in about 80% of the explants. Type-B callus was greener than type-A callus. Callus formation in both the leaf and internodal explants were completely inhibited on the medium used for induction of type-A or type-B callus when the respective medium was supplemented with 54 µM cadmium chloride (Cd). The type-B callus was found to be more resistant (less necrosis and higher relative growth rates) to 27, 54, and 109 µM of Cd than the type-A callus. The type-B callus also exhibited a higher level of peroxidase activity (a marker antioxidant enzyme counteracting oxidative stress) than the type-A callus when cultured on these Cd concentrations. This is the first study showing the importance of callus friability in plant cell response to Cd treatment.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Picloram is one of the potent synthetic auxin useful for the initiation and maintenance of callus, suspension cultures and induction of somatic embryos from various plant tissues (Valverde et al 1987;Hagen et al 1990). The first flower appeared after 2 weeks of transfer of plants on the new medium and ten to fifteen flowers per explant were developed within the next 2 weeks.…”

Section: Regulative Role Of Picloram During In Vitro Floweringmentioning

confidence: 99%

Micropropagation and in vitro flowering of endemic and endangered plant Ceropegia attenuata Hook

Chavan

Nimbalkar

Adsul

et al. 2011

J. Plant Biochem. Biotechnol.

View full text Add to dashboard Cite

Factors affecting in vitro propagation were evaluated for Ceropegia attenuata Hook., an endemic and endangered plant having ornamental potential but a limited reproductive capacity. Rapid shoot multiplication from nodal explants was established using varying concentrations of cytokinins and auxins either alone or in combinations. The highest frequency of shoot induction was achieved when nodal explants were inoculated on Murashige and Skoog (MS) medium supplemented with 13.31 μM 6-benzylaminopurine with a mean of 12.9±0.5 shoots per explant. High concentrations of TDZ (6.81-11.35 μM) and KN (6.78-11.61 μM) resulted in stunted and vitrified shoots. Factors implicated in the promotion of floral transition of the C. attenuata have been identified which are 4-amino-3, 5, 6-trichloropicolinic acid (picloram), 6-benzylaminopurine, sucrose and photoperiod. The highest frequency of flowering (100%) was obtained when axillary shoot explants were transferred to MS medium supplemented with picloram (4.14 μM) within 4 weeks of culture. Transfer of in vitro regenerated shoots to half strength MS medium with 2.46 μM indole-3-butyric acid (IBA) showed maximum root induction. The in vitro grown plantlets were successfully acclimatized in the glasshouse with 85% of survival and showed normal development. The developed protocol provided a simple, cost-effective approach for the conservation of endangered plant C. attenuata for replenishing its declining populations.

show abstract

“…Picloram is a potent auxin useful for the initiation and maintenance of callus and suspension cultures from certain plant tissues (Kefford & Caso 1966;Huang & Chi 1988;Hagen et al 1990). While the stability of some auxins during cell-free incubation has been investigated, there have been no similar tests with picloram.…”

Section: Introductionmentioning

confidence: 99%

Stability and utilization of picloram, vitamins, and sucrose in a tissue culture medium

Hagen

Muneta

Augustin

et al. 1991

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

Nicotinic acid, pyridoxine, and picloram were stable in a liquid MS culture medium (pH 5.5-5.6) during autoclaving and during cell-free incubation in the dark at 5°C or 25°C for up to 6 weeks. Thiamine loss under the same conditions was 16% at 5°C and 18% at 25°C. Five percent of the sucrose in the liquid medium was hydrolyzed during autoclaving. During cell-free incubation in the light (100 #E m 2 s ~) at 25°C, pyridoxine was not detected after 6 days, while 78% of the picloram and 56% of the thiamine were degraded after 6 weeks. All of the niacin and pyridoxine, 13 % of the picloram and 42% of the thiamine in a liquid MS culture medium were utilized in 4 days by potato (cv. Lemhi Russet) tuber suspension cultures growing in the dark at 25°C.

show abstract

Initiation and culture of potato tuber callus tissue with picloram

Cited by 10 publications

References 12 publications

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

Micropropagation and in vitro flowering of endemic and endangered plant Ceropegia attenuata Hook

Stability and utilization of picloram, vitamins, and sucrose in a tissue culture medium

Contact Info

Product

Resources

About